BBa_K2013015 1 BBa_K2013015 protocatechuate decarboxylase 2016-10-11T11:00:00Z 2016-10-15T08:14:45Z This sequence is from Escherichia coli strain K-12 NEB 5-alpha This part contains the sequence of coding protocatechuate decarboxylase which catalyzes protocatechuate into catechol. Experimental Validation This part is validated through Four ways: amplification, PCR, Enzyme cutting and Sequence. Amplification Enzyme:KOD Primer-F:5′-GAATTCGCGGCCGCTTCTAGAGTACTAGAGTCACACATGAGGGTACTAGATG-3′ Primer-R:5′- CGCTACTAGTATTATTAGCTTTGCAGGTAAACCAC-3′ Results PCR Enzyme:Taq Primer-F:5′-CCACCTGACGTCTAAGAAAC-3′ Primer-R:5′-GTATTACCGCCTTTGAGTGA-3′ Results Double digestion After the assembly ,the plasmid was transferred into the Competent E. coli top10. After culturing overnight in LB,we minipreped the plasmid for double digestion .The first cutting procedure was performed with EcoRI and NcoI restriction endonuclease. The second cutting procedure was performed with PstI and NcoI restriction endonuclease.The plasmid was cutted in a 25μL system at 37 ℃ for 1 hours. The Electrophoresis was performed on a 1％ Agarose glu Results false false _2480_ 32794 32794 9 false This is a Translational units part that encodes protocatechuate decarboxylase false Demin Xu annotation2496308 1 protocatechuate decarboxylase range2496308 1 20 1462 annotation2496010 1 RBS range2496010 1 1 13 BBa_K2013015_sequence 1 tcacacatgagggtactagatgagcgtgccggttcagcacccgatgtatatcgacggtcaattcgtgacctggcgtggcgacgcgtggattgatgtggttaacccggcgaccgaagcggttatcagccgtattccggacggtcaggcggaggatgcgcgtaaggcgatcgatgcggcggaacgtgcgcaaccggagtgggaagcgctgccggcgattgagcgtgcgagctggctgcgtaagatcagcgcgggtattcgtgagcgtgcgagcgaaatcagcgcgctgattgtggaggaaggtggcaaaatccagcaactggcggaagttgaggtggcgtttaccgcggactatattgattacatggcggaatgggcgcgtcgttacgagggtgaaatcattcagagcgaccgtccgggcgagaacatcctgctgttcaaacgtgcgctgggtgttaccaccggcatcctgccgtggaactttccgttctttctgattgcgcgtaaaatggcgccggcgctgctgaccggtaacatcattgtgatcaagccgagcgagttcaccccgaacaacgcgattgcgtttgcgaaaatcgttgatgaaattggtctgccgcgtggcgtgttcaacctggttctgggtcgtggcgaaaccgtgggtcaggaactggcgggcaacccgaaggtggcgatggttagcatgaccggtagcgttagcgcgggcgagaaaatcatggcgaccgcggcgaaaaacattaccaaggtgtgcctggaactgggtggcaaggcgccggcgatcgttatggacgatgcggacctggagctggcggtgaaagcgatcgttgatagccgtgtgattaacagcggtcaagtgtgcaactgcgcggaacgtgtgtatgttcagaagggcatttacgaccaattcgtgaaccgtctgggcgaggcgatgcaggcggttcaatttggcaacccggcggaacgtaacgatatcgcgatgggtccgctgattaacgcggcggcgctggagcgtgttgaacagaaagtggcgcgtgcggttgaggaaggcgcgcgtgtggcgttcggtggcaaagcggttgaaggcaagggctactattacccgccgaccctgctgctggacgtgcgtcaagagatgagcatcatgcacgaggaaaccttcggtccggttctgccggtggttgcgtttgacaccctggaggatgcgatcagcatggcgaacgacagcgattatggcctgaccagcagcatttacacccagaacctgaacgttgcgatgaaggcgatcaaaggtctgaagtttggcgaaacctatattaaccgtgagaacttcgaagcgatgcaaggttttcacgcgggctggcgtaaaagcggtatcggtggcgcggatggcaagcacggcctgcacgagtacctgcaaacccaagtggtttacctgcaaagctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z