LOCUS       BBa_K2014000            1072 bp    DNA     linear       05-Dec-2025
DEFINITION  pBAD-E15'UTR->sfGFP
ACCESSION   BBa_K2014000
VERSION     BBa_K2014000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..16
                     /label=O2 region
     misc_feature    158..179
                     /label=O1 region
     misc_feature    205..247
                     /label=I1, I2 binding site 
     misc_feature    269..273
                     /label=-10 signal
     misc_feature    284..322
                     /label=E1-5'UTR
     CDS             323..1072
                     /label=htsfGFP
     misc_feature    202..212
                     /label=CAP binding site
     misc_feature    245..249
                     /label=-35 signal
     RBS             298..306
                     /label=2nd RBS
     RBS             310..315
                     /label=RBS
     misc_feature    323..352
                     /label=His tag
ORIGIN
        1 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct
       61 cgctaaccca accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag
      121 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt
      181 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
      241 ctacctgacg ctttttatcg caactctcta ctgtttctcc ataagaaata attttgttta
      301 actttaagaa ggagatatat ccatgggcag cagccatcat catcatcatc atatgcgtaa
      361 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
      421 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
      481 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac
      541 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
      601 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
      661 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat
      721 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata
      781 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc
      841 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
      901 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac
      961 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
     1021 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaataat aa
//