LOCUS       BBa_K2014001             924 bp    DNA     linear       27-Dec-2024
DEFINITION  prha1-E15'UTR->htsfGFP
ACCESSION   BBa_K2014001
VERSION     BBa_K2014001.1  
FEATURES             Location/Qualifiers
     misc_feature    136..174
                     /label=E1 
     CDS             177..924
                     /label=ht-sfGFP
     misc_feature    1..21
                     /label=CRP binding site
     misc_feature    32..53
                     /label=CRP binding site
     misc_feature    54..70
                     /label=RhaS binding site
     misc_feature    100..105
                     /label=-35 signal
     misc_feature    124..129
                     /label=-10 signal
     RBS             150..158
                     /label=2nd RBS
     RBS             162..167
                     /label=RBS
     misc_feature    175..204
                     /label=HisTag
ORIGIN
        1 gcggtgagca tcacatcacc acaattcagc aaattgtgaa catcatcacg ttcatctttc
       61 cctggttgcc aatggcccat tttcctgtca gtaacgagaa ggtcgcgaat ttaggcgctt
      121 tttagactgg tcgtaagaaa taattttgtt taactttaag aaggagatat atccatgggc
      181 agcagccatc atcatcatca tcatatgcgt aaaggcgaag agctgttcac tggtgtcgtc
      241 cctattctgg tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag
      301 ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa
      361 ctgccggtac cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct
      421 cgttatccgg accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat
      481 gtgcaggaac gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg
      541 aaatttgaag gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa
      601 gacggcaata tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc
      661 accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag
      721 gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct
      781 gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac
      841 gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt
      901 atggatgaac tgtacaaata ataa
//