BBa_K2014001 1 BBa_K2014001 prha1-E15'UTR->htsfGFP 2016-10-10T11:00:00Z 2016-10-14T07:21:45Z Rhamnose induced promoter is a modified part of Escherichia coli genome. 5'UTR is synthetic, similar to a transcript of T7 bacteriophage,sfGFP is synthetic, based on the publication of Jean-Denis Pedelacq et all (2006). We provide a very efficient expression system: tightly controlled and giving a high level of protein expression upon induction by rhamnose. false false _2481_ 27987 25372 9 false We were looking for the shortest, functional rhamnose induced promoter and for the best performing and universal 5'UTR sequence for several promoters of our multipromoter expression system. false Przemyslaw Nuc annotation2504811 1 RhaS binding site range2504811 1 54 70 annotation2504801 1 CRP binding site range2504801 1 32 53 annotation2504819 1 RBS range2504819 1 162 167 annotation2504833 1 HisTag range2504833 1 175 204 annotation2504814 1 -35 signal range2504814 1 100 105 annotation2504700 1 CRP binding site range2504700 1 1 21 annotation2504817 1 2nd RBS range2504817 1 150 158 annotation2504815 1 -10 signal range2504815 1 124 129 annotation2504683 1 ht-sfGFP range2504683 1 177 924 annotation2504682 1 E1 range2504682 1 136 174 BBa_K2014001_sequence 1 gcggtgagcatcacatcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaatttaggcgctttttagactggtcgtaagaaataattttgtttaactttaagaaggagatatatccatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z