LOCUS       BBa_K2014002             881 bp    DNA     linear       26-Nov-2024
DEFINITION  pxylS-E1_5'UTR->sfGFP
ACCESSION   BBa_K2014002
VERSION     BBa_K2014002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=CRP binding site
     misc_feature    20..34
                     /label=XylR binding site
     misc_feature    40..55
                     /label=XylR binding site
     misc_feature    56..60
                     /label=-35 signal
     misc_feature    79..84
                     /label=-10 signal
     misc_feature    90..131
                     /label=5'UTR E1
     RBS             107..115
                     /label=2nd RBS
     RBS             119..124
                     /label=RBS
     misc_feature    132..161
                     /label=His-tag
     CDS             132..881
                     /label=ht-sfGFP
ORIGIN
        1 tgcgagcgag cgcacacttg tgaattatct caatagcagt gtgaaataac ataattgagc
       61 aactgaaagg gagtgcccaa tattacgaca tcagaaataa ttttgtttaa ctttaagaag
      121 gagatatatc catgggcagc agccatcatc atcatcatca tatgcgtaaa ggcgaagagc
      181 tgttcactgg tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt
      241 tttccgtgcg tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca
      301 tctgtactac tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttatg
      361 gtgttcagtg ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg
      421 ccatgccgga aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca
      481 aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt gagctgaaag
      541 gcattgactt taaagaagac ggcaatatcc tgggccataa gctggaatac aattttaaca
      601 gccacaatgt ttacatcacc gccgataaac aaaaaaatgg cattaaagcg aattttaaaa
      661 ttcgccacaa cgtggaggat ggcagcgtgc agctggctga tcactaccag caaaacactc
      721 caatcggtga tggtcctgtt ctgctgccag acaatcacta tctgagcacg caaagcgttc
      781 tgtctaaaga tccgaacgag aaacgcgatc atatggttct gctggagttc gtaaccgcag
      841 cgggcatcac gcatggtatg gatgaactgt acaaataata a
//