BBa_K2014002 1 BBa_K2014002 pxylS-E1_5'UTR->sfGFP 2016-10-11T11:00:00Z 2016-10-14T02:29:09Z Xylose promoter's sequence comes from E.coli genome, E1 enhancer comes from gene 10 of bacteroophage T7. pxylS-E1_5'UTR construct assembly was performed based on XylS promoter (Bba_K1741009). XylS consist only XylA part of the wild-type xylose promoter version XylWT (Bba_K1741007). pxylS-E1_5'UTR has an E1 enhancer introduced into promoter???s 5???UTR. E1 is an RBS from gene 10 of bacteriophage T7. This promoter is induced by xylose (0.4%), which starts the transcription of the ORF. Construct contains his-tagged sfGFP protein. false false _2481_ 27987 28000 9 false We started working on xylose promoters, because of the cheap inducer-xylose and potentially tight control of expression. Modification of E1-enhancer in 5'UTR gives the highest protein expression levels. false Daria Niewiadomska annotation2509299 1 CRP binding site range2509299 1 1 19 annotation2509302 1 -35 signal range2509302 1 56 60 annotation2509304 1 5'UTR E1 range2509304 1 90 131 annotation2509305 1 2nd RBS range2509305 1 107 115 annotation2509307 1 His-tag range2509307 1 132 161 annotation2509308 1 ht-sfGFP range2509308 1 132 881 annotation2509300 1 XylR binding site range2509300 1 20 34 annotation2509306 1 RBS range2509306 1 119 124 annotation2509301 1 XylR binding site range2509301 1 40 55 annotation2509303 1 -10 signal range2509303 1 79 84 BBa_K2014002_sequence 1 tgcgagcgagcgcacacttgtgaattatctcaatagcagtgtgaaataacataattgagcaactgaaagggagtgcccaatattacgacatcagaaataattttgtttaactttaagaaggagatatatccatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z