LOCUS       BBa_K2014003            1059 bp    DNA     linear       23-Dec-2024
DEFINITION  pBAD-M5'UTR->sfGFP
ACCESSION   BBa_K2014003
VERSION     BBa_K2014003.1  
FEATURES             Location/Qualifiers
     misc_feature    284..309
                     /label=M5'UTR
     CDS             310..1059
                     /label=ht-sfGFP
     misc_feature    310..339
                     /label=HisTag
     misc_feature    158..179
                     /label=O1 region 
     misc_feature    269..273
                     /label=-10 signal
     misc_feature    1..16
                     /label=O2 region
     misc_feature    205..247
                     /label=i1-i2
     misc_feature    203..213
                     /label=CAP binding site
     misc_feature    245..249
                     /label=-35 signal
ORIGIN
        1 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct
       61 cgctaaccca accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag
      121 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt
      181 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
      241 ctacctgacg ctttttatcg caactctcta ctgtttctcc atattcaagc aagccaggag
      301 atatatcata tgggcagcag ccatcatcat catcatcata tgcgtaaagg cgaagagctg
      361 ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt
      421 tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc
      481 tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct gacttatggt
      541 gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc
      601 atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa
      661 acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc
      721 attgacttta aagaagacgg caatatcctg ggccataagc tggaatacaa ttttaacagc
      781 cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt
      841 cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca
      901 atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg
      961 tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg
     1021 ggcatcacgc atggtatgga tgaactgtac aaataataa
//