BBa_K2014004
1
BBa_K2014004
pxylS-M5'UTR->sfGFP
2016-10-11T11:00:00Z
2016-10-14T02:17:45Z
Xylose induced promoter is a fragment of E.coli K-12 genome. Synthetic 5'UTR is a synthetic unstructured sequence with well positioned RBS.
Genes involved in catabolism of xylose in E.coli are organized into two transcription units, which drive the transcription in opposite directions, by two promoters - XylA and XylF.
During last iGEM edition, we performed many modifications of the initial promoter. XylA1 promoter (BBa_K1741007) was built by changing the original 5???UTR in XylA promoter for 5???UTR derived from constitutive proD promoter.
The next step was to reduce the promoter???s size by removing its XylF part while keeping the modified 5'UTR (from proD) in XylA part- XylS promoter (BBa_K1741009).
And this promoter became our base for XylS-UTR promoter construction, which consist of XylA part with edited 5???UTR. As above in the Ara1-UTR promoter, here again we replaced 5???UTR with synthetic 5???UTR, derived from Mel2 promoter(BBa_K1741004).
false
false
_2481_
28000
28000
9
false
We decided to work on xylose induced promoter cause this sugar is relatively cheap.
false
Daria Niewiadomska
annotation2506741
1
M 5'UTR
range2506741
1
93
119
annotation2506739
1
His - Tag
range2506739
1
120
149
annotation2506721
1
XylR binding site
range2506721
1
20
34
annotation2506732
1
-10 signal
range2506732
1
79
84
annotation2506729
1
-35 signal
range2506729
1
56
60
annotation2506743
1
ht-sfGFP
range2506743
1
120
869
annotation2506725
1
XylR binding site
range2506725
1
40
55
annotation2506735
1
CRP binding site
range2506735
1
1
19
BBa_K2014004_sequence
1
tgcgagcgagcgcacacttgtgaattatctcaatagcagtgtgaaataacataattgagcaactgaaagggagtgcccaatattacgacatcattcaagcaagccaggagatatatcatatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z