BBa_K2014005 1 BBa_K2014005 AraC-pBAD->sfGFP_B 2016-10-11T11:00:00Z 2016-10-14T02:32:55Z The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence. We were using standard AraC-pBAD promoter to compare the influence of different ORFs structures coding for the same protein sfGFP. false false _2481_ 27987 28000 9 false We were looking for the best method to optimize gene expression in E.coli. false Daria Niewiadomska annotation2506759 1 -35 signal range2506759 1 1153 1157 annotation2506744 1 AraC CDS range2506744 1 1 879 annotation2506754 1 i1-i2 region range2506754 1 1113 1155 annotation2506751 1 O1 region range2506751 1 1066 1087 annotation2506750 1 O2 region range2506750 1 909 924 annotation2506757 1 ht-sfGFP-B range2506757 1 1218 1967 annotation2506756 1 5'UTR range2506756 1 1191 1217 annotation2506761 1 His-Tag range2506761 1 1218 1247 annotation2506760 1 CAP binding site range2506760 1 1111 1121 annotation2506752 1 -10 signal range2506752 1 1177 1181 BBa_K2014005_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacgaaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctgactaatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccaccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttggatggagtgaaacgatgggcagcagccatcatcatcatcatcatatgcgcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggcgatgtgaacggccataaatttagcgtgcgcggcgaaggcgaaggcgatgcgaccaacggcaaactgaccctgaaatttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttgcgcgctatccggatcatatgaaacagcatgatttttttaaaagcgcgatgccggaaggctatgtgcaggaacgcaccattagctttaaagatgatggcacctataaaacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgattttaaagaagatggcaacattctgggccataaactggaatataactttaacagccataacgtgtatattaccgcggataaacagaaaaacggcattaaagcgaactttaaaattcgccataacgtggaagatggcagcgtgcagctggcggatcattatcagcagaacaccccgattggcgatggcccggtgctgctgccggataaccattatctgagcacccagagcgtgctgagcaaagatccgaacgaaaaacgcgatcatatggtgctgctggaatttgtgaccgcggcgggcattacccatggcatggatgaactgtataaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z