LOCUS       BBa_K2014006            1967 bp    DNA     linear       22-Nov-2024
DEFINITION  AraC-pBAD->sfGFP_W
ACCESSION   BBa_K2014006
VERSION     BBa_K2014006.1  
FEATURES             Location/Qualifiers
     misc_feature    909..924
                     /label=O2 region
     misc_feature    1066..1087
                     /label=O1 region
     misc_feature    1113..1155
                     /label=i1-i2 region
     misc_feature    1177..1181
                     /label=-10 signal
     misc_feature    1191..1217
                     /label=5'UTR
     CDS             1..879
                     /label=AraC CDS
     CDS             1218..1967
                     /label=ht-sfGFP-W
     misc_feature    1111..1121
                     /label=CAP binding site
     misc_feature    1153..1157
                     /label=-35 signal
     misc_feature    1218..1247
                     /label=His-Tag
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg
      181 actaatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttggatggag tgaaacgatg ggcagcagcc atcatcatca tcatcatatg aggaagggag
     1261 aggagctatt cacaggagta gtacccatac tagtagagct agacggagac gtaaatggac
     1321 acaagttctc agtaagggga gagggagagg gagacgctac aaatggaaag ctaacactaa
     1381 agttcatatg tacaacagga aagctacccg taccctggcc cacactagta acaacactaa
     1441 catacggagt acaatgtttc gctaggtacc ccgaccacat gaagcaacac gacttcttca
     1501 agtcagctat gcccgaggga tacgtacaag agaggacaat atcattcaag gacgacggaa
     1561 catacaagac aagggctgag gtaaagttcg agggagacac actagtaaat aggatagagc
     1621 taaagggaat agacttcaag gaggacggaa atatactagg acacaagcta gagtacaatt
     1681 tcaattcaca caatgtatac ataacagctg acaagcaaaa gaatggaata aaggctaatt
     1741 tcaagataag gcacaatgta gaggacggat cagtacaact agctgaccac taccaacaaa
     1801 atacacccat aggagacgga cccgtactac tacccgacaa tcactaccta tcaacacaat
     1861 cagtactatc aaaggacccc aatgagaaga gggaccacat ggtactacta gagttcgtaa
     1921 cagctgctgg aataacacac ggaatggacg agctatacaa gtaataa
//