BBa_K2014006 1 BBa_K2014006 AraC-pBAD->sfGFP_W 2016-10-11T11:00:00Z 2016-10-14T10:11:15Z The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence. We were using standard AraC-pBAD promoter to compare the influence of different ORFs structures coding for the same protein sfGFP. false false _2481_ 27987 28000 9 false We were looking for the best method to optimize gene expression in E.coli. false Daria Niewiadomska annotation2506802 1 His-Tag range2506802 1 1218 1247 annotation2506783 1 5'UTR range2506783 1 1191 1217 annotation2506781 1 -10 signal range2506781 1 1177 1181 annotation2506792 1 AraC CDS range2506792 1 1 879 annotation2506773 1 i1-i2 region range2506773 1 1113 1155 annotation2506794 1 ht-sfGFP-W range2506794 1 1218 1967 annotation2506770 1 O2 region range2506770 1 909 924 annotation2506796 1 -35 signal range2506796 1 1153 1157 annotation2506795 1 CAP binding site range2506795 1 1111 1121 annotation2506771 1 O1 region range2506771 1 1066 1087 BBa_K2014006_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacgaaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctgactaatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccaccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttggatggagtgaaacgatgggcagcagccatcatcatcatcatcatatgaggaagggagaggagctattcacaggagtagtacccatactagtagagctagacggagacgtaaatggacacaagttctcagtaaggggagagggagagggagacgctacaaatggaaagctaacactaaagttcatatgtacaacaggaaagctacccgtaccctggcccacactagtaacaacactaacatacggagtacaatgtttcgctaggtaccccgaccacatgaagcaacacgacttcttcaagtcagctatgcccgagggatacgtacaagagaggacaatatcattcaaggacgacggaacatacaagacaagggctgaggtaaagttcgagggagacacactagtaaataggatagagctaaagggaatagacttcaaggaggacggaaatatactaggacacaagctagagtacaatttcaattcacacaatgtatacataacagctgacaagcaaaagaatggaataaaggctaatttcaagataaggcacaatgtagaggacggatcagtacaactagctgaccactaccaacaaaatacacccataggagacggacccgtactactacccgacaatcactacctatcaacacaatcagtactatcaaaggaccccaatgagaagagggaccacatggtactactagagttcgtaacagctgctggaataacacacggaatggacgagctatacaagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z