BBa_K2014007 1 BBa_K2014007 AraC-pBAD->sfGFP_8R 2016-10-11T11:00:00Z 2016-10-12T02:52:53Z The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with 8R tag to check the translation efficiency. We were using standard AraC-pBAD promoter to compare the influence of different ORFs structures coding for the same protein sfGFP and also the stability of the mRNA during translation. false false _2481_ 28000 28000 9 false We were looking for the best method to optimize gene expression in E.coli and wanted to check out if there is any influence of the 8R tag for the mRNA stability. false Daria Niewiadomska annotation2506809 1 O2 region range2506809 1 909 924 annotation2506833 1 8R range2506833 1 1962 1986 annotation2506810 1 O1 region range2506810 1 1066 1087 annotation2506828 1 5'UTR range2506828 1 1191 1217 annotation2506830 1 CAP binding site range2506830 1 1111 1121 annotation2506829 1 ht-sfGFP-8R range2506829 1 1218 1991 annotation2506831 1 -35 signal range2506831 1 1153 1157 annotation2506826 1 AraC CDS range2506826 1 1 879 annotation2506832 1 His-Tag range2506832 1 1218 1247 annotation2506812 1 -10 signal range2506812 1 1177 1181 annotation2506827 1 i1-i2 region range2506827 1 1113 1155 BBa_K2014007_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacgaaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctgactaatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccaccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttggatggagtgaaacgatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaaagaaggagaagaagaagaagaagataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z