BBa_K2014008 1 BBa_K2014008 AraC-pBAD->mRFP_W 2016-10-11T11:00:00Z 2016-10-14T10:22:03Z The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the mRFP permutation, with optimized codon sequence. We were using standard AraC-pBAD promoter to compare the influence of different ORFs structures coding for the same protein mRFP. false false _2481_ 27987 28000 9 false We were looking for the best method to optimize gene expression in E.coli. false Daria Niewiadomska annotation2506868 1 ht-mRFP-W range2506868 1 1218 1928 annotation2506872 1 -35 signal range2506872 1 1153 1157 annotation2506837 1 i1-i2 region range2506837 1 1113 1155 annotation2506835 1 O2 region range2506835 1 909 924 annotation2506852 1 5'UTR range2506852 1 1191 1217 annotation2506871 1 CAP binding site range2506871 1 1111 1121 annotation2506836 1 O1 region range2506836 1 1066 1087 annotation2506866 1 AraC CDS range2506866 1 1 879 annotation2506851 1 -10 signal range2506851 1 1177 1181 BBa_K2014008_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacgaaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctgactaatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccaccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttggatggagtgaaacgatgggcagcagccatcatcatcaccaccacatggcttcatcagaggacgtaataaaggagttcatgagattcaaggtaagaatggagggatcagtaaatggacacgagttcgagatagagggagagggagagggaagaccctacgagggaacacaaacagctaagctaaaggtaacaaagggaggacccctacccttcgcttgggacatactatcaccccaattccaatacggatcaaaggcttacgtaaagcaccccgctgacatacccgactacctaaagctatcattccccgagggattcaagtgggagagagtaatgaatttcgaggacggaggagtagtaaccgtaacacaagactcatcactacaagacggagagttcatatacaaggtaaagctaagaggaacaaatttcccctcagacggacccgtaatgcaaaagaagacaatgggatgggaggcttcaacagagagaatgtaccccgaggacggagctctaaagggagagataaagatgagactaaagctaaaggacggaggacactacgacgctgaggtaaagacaacatacatggctaagaagcccgtacaactacccggagcttacaagacagacataaagctagacataacatcacacaatgaggactacacaatagtagagcaatacgagagagctgagggaagacactcaacaggagcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z