LOCUS       BBa_K2014009             881 bp    DNA     linear       23-Dec-2024
DEFINITION  pxylS-E1_5'UTR->sfGFP_B
ACCESSION   BBa_K2014009
VERSION     BBa_K2014009.1  
FEATURES             Location/Qualifiers
     misc_feature    20..34
                     /label=XylR binding site
     misc_feature    40..55
                     /label=XylR binding site
     misc_feature    56..60
                     /label=-35 signal
     misc_feature    79..84
                     /label=-10 signal
     misc_feature    1..19
                     /label=CRP binding site
     misc_feature    90..131
                     /label=5'UTR E1
     CDS             132..881
                     /label=ht-sfGFP-B
     RBS             107..115
                     /label=2nd RBS
     RBS             119..124
                     /label=RBS
     misc_feature    132..161
                     /label=His-Tag
ORIGIN
        1 tgcgagcgag cgcacacttg tgaattatct caatagcagt gtgaaataac ataattgagc
       61 aactgaaagg gagtgcccaa tattacgaca tcagaaataa ttttgtttaa ctttaagaag
      121 gagatatatc catgggcagc agccatcatc atcatcatca tatgcgcaaa ggcgaagaac
      181 tgtttaccgg cgtggtgccg attctggtgg aactggatgg cgatgtgaac ggccataaat
      241 ttagcgtgcg cggcgaaggc gaaggcgatg cgaccaacgg caaactgacc ctgaaattta
      301 tttgcaccac cggcaaactg ccggtgccgt ggccgaccct ggtgaccacc ctgacctatg
      361 gcgtgcagtg ctttgcgcgc tatccggatc atatgaaaca gcatgatttt tttaaaagcg
      421 cgatgccgga aggctatgtg caggaacgca ccattagctt taaagatgat ggcacctata
      481 aaacccgcgc ggaagtgaaa tttgaaggcg ataccctggt gaaccgcatt gaactgaaag
      541 gcattgattt taaagaagat ggcaacattc tgggccataa actggaatat aactttaaca
      601 gccataacgt gtatattacc gcggataaac agaaaaacgg cattaaagcg aactttaaaa
      661 ttcgccataa cgtggaagat ggcagcgtgc agctggcgga tcattatcag cagaacaccc
      721 cgattggcga tggcccggtg ctgctgccgg ataaccatta tctgagcacc cagagcgtgc
      781 tgagcaaaga tccgaacgaa aaacgcgatc atatggtgct gctggaattt gtgaccgcgg
      841 cgggcattac ccatggcatg gatgaactgt ataaataata a
//