BBa_K2014009 1 BBa_K2014009 pxylS-E1_5'UTR->sfGFP_B 2016-10-11T11:00:00Z 2016-10-14T10:32:27Z The xylS promoter is a modified version of xylR-xylF-xylA1 fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence. We were using xylS promoter to compare the influence of different ORFs structures coding for the same protein sfGFP. false false _2481_ 27987 28000 9 false We were looking for the best method to optimize gene expression in E.coli. false Daria Niewiadomska annotation2506900 1 His-Tag range2506900 1 132 161 annotation2506890 1 ht-sfGFP-B range2506890 1 132 881 annotation2506887 1 -10 signal range2506887 1 79 84 annotation2506899 1 RBS range2506899 1 119 124 annotation2506878 1 XylR binding site range2506878 1 20 34 annotation2506898 1 2nd RBS range2506898 1 107 115 annotation2506889 1 5'UTR E1 range2506889 1 90 131 annotation2506879 1 XylR binding site range2506879 1 40 55 annotation2506888 1 CRP binding site range2506888 1 1 19 annotation2506886 1 -35 signal range2506886 1 56 60 BBa_K2014009_sequence 1 tgcgagcgagcgcacacttgtgaattatctcaatagcagtgtgaaataacataattgagcaactgaaagggagtgcccaatattacgacatcagaaataattttgtttaactttaagaaggagatatatccatgggcagcagccatcatcatcatcatcatatgcgcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggcgatgtgaacggccataaatttagcgtgcgcggcgaaggcgaaggcgatgcgaccaacggcaaactgaccctgaaatttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttgcgcgctatccggatcatatgaaacagcatgatttttttaaaagcgcgatgccggaaggctatgtgcaggaacgcaccattagctttaaagatgatggcacctataaaacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgattttaaagaagatggcaacattctgggccataaactggaatataactttaacagccataacgtgtatattaccgcggataaacagaaaaacggcattaaagcgaactttaaaattcgccataacgtggaagatggcagcgtgcagctggcggatcattatcagcagaacaccccgattggcgatggcccggtgctgctgccggataaccattatctgagcacccagagcgtgctgagcaaagatccgaacgaaaaacgcgatcatatggtgctgctggaatttgtgaccgcggcgggcattacccatggcatggatgaactgtataaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z