LOCUS       BBa_K2014010             881 bp    DNA     linear       23-Dec-2024
DEFINITION  pxylS-E1_5'UTR->sfGFP_W
ACCESSION   BBa_K2014010
VERSION     BBa_K2014010.1  
FEATURES             Location/Qualifiers
     misc_feature    20..34
                     /label=XylR binding site
     misc_feature    40..55
                     /label=XylR binding site
     misc_feature    56..60
                     /label=-35 signal
     misc_feature    79..84
                     /label=-10 signal
     misc_feature    1..19
                     /label=CRP binding site
     misc_feature    90..131
                     /label=5'UTR E1
     CDS             132..881
                     /label=ht-sfGFP-W
     RBS             107..115
                     /label=2nd RBS
     RBS             119..124
                     /label=RBS
     misc_feature    132..161
                     /label=His-Tag
ORIGIN
        1 tgcgagcgag cgcacacttg tgaattatct caatagcagt gtgaaataac ataattgagc
       61 aactgaaagg gagtgcccaa tattacgaca tcagaaataa ttttgtttaa ctttaagaag
      121 gagatatatc catgggcagc agccatcatc atcatcatca tatgaggaag ggagaggagc
      181 tattcacagg agtagtaccc atactagtag agctagacgg agacgtaaat ggacacaagt
      241 tctcagtaag gggagaggga gagggagacg ctacaaatgg aaagctaaca ctaaagttca
      301 tatgtacaac aggaaagcta cccgtaccct ggcccacact agtaacaaca ctaacatacg
      361 gagtacaatg tttcgctagg taccccgacc acatgaagca acacgacttc ttcaagtcag
      421 ctatgcccga gggatacgta caagagagga caatatcatt caaggacgac ggaacataca
      481 agacaagggc tgaggtaaag ttcgagggag acacactagt aaataggata gagctaaagg
      541 gaatagactt caaggaggac ggaaatatac taggacacaa gctagagtac aatttcaatt
      601 cacacaatgt atacataaca gctgacaagc aaaagaatgg aataaaggct aatttcaaga
      661 taaggcacaa tgtagaggac ggatcagtac aactagctga ccactaccaa caaaatacac
      721 ccataggaga cggacccgta ctactacccg acaatcacta cctatcaaca caatcagtac
      781 tatcaaagga ccccaatgag aagagggacc acatggtact actagagttc gtaacagctg
      841 ctggaataac acacggaatg gacgagctat acaagtaata a
//