BBa_K2014010 1 BBa_K2014010 pxylS-E1_5'UTR->sfGFP_W 2016-10-11T11:00:00Z 2016-10-14T10:37:34Z We used one of the sfGFP permutation, with optimized codon sequence. We were using xylS promoter to compare the influence of different ORFs structures coding for the same protein sfGFP. The xylS promoter is a modified version of xylR-xylF-xylA promoter from E.coli K-12 genome. false false _2481_ 27987 28000 9 false We were looking for the best method to optimize gene expression in E.coli. false Daria Niewiadomska annotation2506910 1 -35 signal range2506910 1 56 60 annotation2506916 1 ht-sfGFP-W range2506916 1 132 881 annotation2506914 1 5'UTR E1 range2506914 1 90 131 annotation2506921 1 His-Tag range2506921 1 132 161 annotation2506912 1 -10 signal range2506912 1 79 84 annotation2506913 1 CRP binding site range2506913 1 1 19 annotation2506908 1 XylR binding site range2506908 1 20 34 annotation2506918 1 RBS range2506918 1 119 124 annotation2506917 1 2nd RBS range2506917 1 107 115 annotation2506909 1 XylR binding site range2506909 1 40 55 BBa_K2014010_sequence 1 tgcgagcgagcgcacacttgtgaattatctcaatagcagtgtgaaataacataattgagcaactgaaagggagtgcccaatattacgacatcagaaataattttgtttaactttaagaaggagatatatccatgggcagcagccatcatcatcatcatcatatgaggaagggagaggagctattcacaggagtagtacccatactagtagagctagacggagacgtaaatggacacaagttctcagtaaggggagagggagagggagacgctacaaatggaaagctaacactaaagttcatatgtacaacaggaaagctacccgtaccctggcccacactagtaacaacactaacatacggagtacaatgtttcgctaggtaccccgaccacatgaagcaacacgacttcttcaagtcagctatgcccgagggatacgtacaagagaggacaatatcattcaaggacgacggaacatacaagacaagggctgaggtaaagttcgagggagacacactagtaaataggatagagctaaagggaatagacttcaaggaggacggaaatatactaggacacaagctagagtacaatttcaattcacacaatgtatacataacagctgacaagcaaaagaatggaataaaggctaatttcaagataaggcacaatgtagaggacggatcagtacaactagctgaccactaccaacaaaatacacccataggagacggacccgtactactacccgacaatcactacctatcaacacaatcagtactatcaaaggaccccaatgagaagagggaccacatggtactactagagttcgtaacagctgctggaataacacacggaatggacgagctatacaagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z