BBa_K2014011 1 BBa_K2014011 pxylF-xylA->sfGFP_B 2016-10-11T11:00:00Z 2016-10-14T10:53:23Z The xylF-xylA promoter is a fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence. We were using xylF-xylA promoter to compare the influence of different ORFs structures coding for the same protein sfGFP. false false _2481_ 27987 28000 9 false We were looking for the best method to optimize gene expression in E.coli. false Daria Niewiadomska annotation2507042 1 His-Tag range2507042 1 366 395 annotation2506999 1 IF2 range2506999 1 103 118 annotation2507017 1 XylR binding site range2507017 1 274 289 annotation2506956 1 -10 signal range2506956 1 69 74 annotation2507027 1 -10 signal range2507027 1 313 318 annotation2507031 1 IF1 range2507031 1 123 138 annotation2507035 1 5'UTR range2507035 1 324 365 annotation2507036 1 ht-sfGFP-B range2507036 1 366 1115 annotation2507021 1 -35 signal range2507021 1 290 294 annotation2507034 1 CRP binding site range2507034 1 235 253 annotation2507030 1 5'UTR range2507030 1 1 62 annotation2506957 1 -35 signal range2506957 1 91 95 annotation2507016 1 XylR binding site range2507016 1 254 268 BBa_K2014011_sequence 1 ggtgtagggccttctgtagttagaggacagttttaataagtaacaatcaccgcgataaacgtaaccaatttttagcaactaaacaggggaaaacaattacagatttttatctttcgattacgatttttggtttatttcttgatttatgaccgagatcttacttttgttgcgcaattgtacttattgcatttttctcttcgaggaattacccagtttcatcattccattttattttgcgagcgagcgcacacttgtgaattatctcaatagcagtgtgaaataacataattgagcaactgaaagggagtgcccaatattacgacatcatccatcacccgcggcattacctgattatggagttcaatatgggcagcagccatcatcatcatcatcatatgcgcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggcgatgtgaacggccataaatttagcgtgcgcggcgaaggcgaaggcgatgcgaccaacggcaaactgaccctgaaatttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttgcgcgctatccggatcatatgaaacagcatgatttttttaaaagcgcgatgccggaaggctatgtgcaggaacgcaccattagctttaaagatgatggcacctataaaacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgattttaaagaagatggcaacattctgggccataaactggaatataactttaacagccataacgtgtatattaccgcggataaacagaaaaacggcattaaagcgaactttaaaattcgccataacgtggaagatggcagcgtgcagctggcggatcattatcagcagaacaccccgattggcgatggcccggtgctgctgccggataaccattatctgagcacccagagcgtgctgagcaaagatccgaacgaaaaacgcgatcatatggtgctgctggaatttgtgaccgcggcgggcattacccatggcatggatgaactgtataaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z