BBa_K2014012 1 BBa_K2014012 pxylF-xylA->sfGFP_W 2016-10-11T11:00:00Z 2016-10-14T07:26:29Z The xylF-xylA promoter is a modified version of xylR-xylF-xylA promoter present in E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence. We were using pxylF-xylA promoter to compare the influence of different ORFs structures coding for the same protein sfGFP. false false _2481_ 28000 28000 9 false We were looking for the best method to optimize gene expression in E.coli. false Daria Niewiadomska annotation2504820 1 IF2 range2504820 1 103 118 annotation2504818 1 -35 signal range2504818 1 91 95 annotation2504812 1 5"UTR range2504812 1 1 62 annotation2504848 1 5'UTR range2504848 1 324 365 annotation2504851 1 ht-sfGFP range2504851 1 366 1115 annotation2504850 1 His tag range2504850 1 366 395 annotation2504847 1 -10 range2504847 1 318 318 annotation2504838 1 -35 range2504838 1 290 294 annotation2504830 1 IF1 range2504830 1 123 138 annotation2504834 1 XylR binding site range2504834 1 254 268 annotation2504837 1 XylR bidnig site range2504837 1 274 289 annotation2504832 1 CRP binding site range2504832 1 235 253 annotation2504816 1 -10 signal range2504816 1 69 74 BBa_K2014012_sequence 1 ggtgtagggccttctgtagttagaggacagttttaataagtaacaatcaccgcgataaacgtaaccaatttttagcaactaaacaggggaaaacaattacagatttttatctttcgattacgatttttggtttatttcttgatttatgaccgagatcttacttttgttgcgcaattgtacttattgcatttttctcttcgaggaattacccagtttcatcattccattttattttgcgagcgagcgcacacttgtgaattatctcaatagcagtgtgaaataacataattgagcaactgaaagggagtgcccaatattacgacatcatccatcacccgcggcattacctgattatggagttcaatatgggcagcagccatcatcatcatcatcatatgaggaagggagaggagctattcacaggagtagtacccatactagtagagctagacggagacgtaaatggacacaagttctcagtaaggggagagggagagggagacgctacaaatggaaagctaacactaaagttcatatgtacaacaggaaagctacccgtaccctggcccacactagtaacaacactaacatacggagtacaatgtttcgctaggtaccccgaccacatgaagcaacacgacttcttcaagtcagctatgcccgagggatacgtacaagagaggacaatatcattcaaggacgacggaacatacaagacaagggctgaggtaaagttcgagggagacacactagtaaataggatagagctaaagggaatagacttcaaggaggacggaaatatactaggacacaagctagagtacaatttcaattcacacaatgtatacataacagctgacaagcaaaagaatggaataaaggctaatttcaagataaggcacaatgtagaggacggatcagtacaactagctgaccactaccaacaaaatacacccataggagacggacccgtactactacccgacaatcactacctatcaacacaatcagtactatcaaaggaccccaatgagaagagggaccacatggtactactagagttcgtaacagctgctggaataacacacggaatggacgagctatacaagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z