BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2002
1
-10
range2002
1
43
48
annotation2001
1
lac O1
range2001
1
26
42
annotation2000
1
-35
range2000
1
20
25
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation1999
1
lac O1
range1999
1
3
19
BBa_K2015001
1
P11-4
P11-4
2016-10-13T11:00:00Z
2016-10-21T08:54:53Z
idt
P11-4
false
false
_2482_
28306
28357
9
false
a
false
Kazuhiro Mimata, Ruka Nishimura
annotation2522674
1
P11-4
range2522674
1
1
33
BBa_K2015007
1
BBa_K2015007
RADA for multi
2016-10-13T11:00:00Z
2016-10-14T08:50:07Z
idt
RADA for multi
true
false
_2482_
28932
28357
9
false
a
false
Kazuhiro Mimata
component2505986
1
BBa_K2015001
component2505981
1
BBa_R0011
annotation2505986
1
BBa_K2015001
range2505986
1
64
96
annotation2505981
1
BBa_R0011
range2505981
1
1
54
BBa_K2015007_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagcagcaacgcttcgagtgggagttcgaacaacag
BBa_K2015001_sequence
1
caacagcgttttgaatgggagtttgagcaacaa
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z