LOCUS       BBa_K2015012            1435 bp    DNA     linear       22-Dec-2024
DEFINITION  LacI + promoter + RBS
ACCESSION   BBa_K2015012
VERSION     BBa_K2015012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    1152..1190
                     /label=LVA
     CDS             63..1190
                     /label=lacI-LVA
     misc_feature    63..1190
                     /label=BBa_C0012
     misc_feature    1191..1215
                     /label=Help:Barcodes
     CDS             63..1190
                     /label=BBa_C0012
     stem_loop       1235..1278
                     /label=stem_loop
     misc_feature    1224..1303
                     /label=BBa_B0010
     terminator      1224..1303
                     /label=BBa_B0010
     stem_loop       1319..1338
                     /label=T7 TE
     misc_feature    1345..1345
                     /label=stop
     polyA_site      1339..1352
                     /label=polya
     misc_feature    1312..1352
                     /label=BBa_B0012
     terminator      1312..1352
                     /label=BBa_B0012
     terminator      1224..1352
                     /label=BBa_B0015
     misc_feature    1363..1379
                     /label=lac O1
     promoter        1380..1385
                     /label=-35
     misc_feature    1386..1402
                     /label=lac O1
     promoter        1403..1408
                     /label=-10
     misc_feature    1361..1414
                     /label=BBa_R0011
     promoter        1361..1414
                     /label=BBa_R0011
     misc_feature    1428..1431
                     /label=conserved
     RBS             1424..1435
                     /label=BBa_B0034
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
      121 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
      181 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
      241 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
      301 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
      361 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
      421 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
      481 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
      541 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
      601 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
      661 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
      721 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
      781 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
      841 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
      901 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
      961 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     1021 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     1081 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1141 aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
     1201 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1261 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1321 ctcaccttcg ggtgggcctt tctgcgttta tatactagag aattgtgagc ggataacaat
     1381 tgacattgtg agcggataac aagatactga gcacatacta gagaaagagg agaaa
//