LOCUS       BBa_K2017000            2358 bp    DNA     linear       09-Apr-2025
DEFINITION  C-split Cas9 + DnaE C-intein
ACCESSION   BBa_K2017000
VERSION     BBa_K2017000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..108
                     /label=DnaE C-intein
     CDS             109..2358
                     /label=C-split Cas9
ORIGIN
        1 atgataaaga tagccacgcg aaagtacctt ggcaagcaaa atgtttatga tattggagtt
       61 gagagggatc ataacttcgc tttgaagaac ggatttatag ccagcaacac ttacgctcat
      121 ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg
      181 ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat
      241 tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct
      301 ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga cagtctccac
      361 gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt
      421 aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc
      481 gagatggccc gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg
      541 aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt
      601 gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac
      661 atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt ggatcatatc
      721 gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac aagatccgat
      781 aaaaatagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat
      841 tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact
      901 aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt
      961 gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc
     1021 aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag
     1081 ctggtttcag atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac
     1141 caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat
     1201 cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg
     1261 atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat
     1321 attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca
     1381 cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg
     1441 acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag
     1501 accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca
     1561 cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac
     1621 agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag
     1681 gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt
     1741 ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac
     1801 tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag
     1861 aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac
     1921 tatgaaaagc tcaaaggatc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa
     1981 cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc
     2041 ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc
     2101 atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct
     2161 gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag
     2221 gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac
     2281 ctctctcagc tcggtggaga cagcagggct gaccccaaga agaagaggaa ggtgcccaag
     2341 aagaagagga aggtgtga
//