LOCUS       BBa_K2017001            2241 bp    DNA     linear       03-Apr-2025
DEFINITION  N-split Cas9 + DnaE N-intein
ACCESSION   BBa_K2017001
VERSION     BBa_K2017001.1  
FEATURES             Location/Qualifiers
     CDS             1..1933
                     /label=N-split Cas9
     misc_feature    1934..2241
                     /label=DnaE N-intein
ORIGIN
        1 atgcccaaga agaagaggaa ggtgtgagac aagaagtact ccattgggct cgatatcggc
       61 acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc
      121 aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg
      181 ttcgactccg gggaaacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat
      241 acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag
      301 gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag
      361 cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac
      421 ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg
      481 ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg
      541 gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac
      601 aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg
      661 agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag
      721 aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt
      781 aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat
      841 gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg
      901 gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc
      961 accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg
     1021 actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc
     1081 gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt
     1141 tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag
     1201 cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac
     1261 cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt
     1321 ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta
     1381 ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagact
     1441 atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc
     1501 gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct
     1561 ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa
     1621 gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc
     1681 ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagattattt caaaaagatt
     1741 gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga
     1801 acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac
     1861 gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt
     1921 gaagaacgct tgaaatgctt atcttacgaa acagagattc tgactgtgga gtacgggctg
     1981 ttacccattg ggaaaattgt cgagaaacga atagaatgta ccgtgtattc cgttgataac
     2041 aacggcaaca tatacacgca gccagtcgct cagtggcacg atagagggga acaagaggtc
     2101 tttgagtatt gcctcgaaga tggaagtctt atccgtgcaa cgaaggacca caagtttatg
     2161 actgtggatg ggcaaatgtt acctatcgac gagatctttg agcgtgagtt agatttgatg
     2221 agggtggaca acctccctaa c
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