LOCUS BBa_K2018000 4271 bp DNA linear 25-Nov-2024 DEFINITION phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB. ACCESSION BBa_K2018000 VERSION BBa_K2018000.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23104 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1..55 /label=BBa_K608002 CDS 416..2185 /label=phaC1 CDS 2270..3451 /label=phaA CDS 3526..4266 /label=phaB1 promoter 64..113 /label=Pwt RBS 399..405 /label=RBS RBS 2259..2262 /label=RBS RBS 3516..3519 /label=RBS misc_feature 64..4271 /label=BBa_K934001 ORIGIN 1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta 61 gaggattccc gcattgacag cgcgtgcgtt gcaaggcaac aatggactca aatgtctcgg 121 aatcgctgac gattcccagg tttctccggc aagcatagcg catggcgtct ccatgcgaga 181 atgtcgcgct tgccggataa aaggggagcc gctatcggaa tggacgcaag ccacggccgc 241 agcaggtgcg gtcgagggct tccagccagt tccagggcag atgtgccggc agaccctccc 301 gctttggggg aggcgcaagc cgggtccatt cggatagcat ctccccatgc aaagtgccgg 361 ccagggcaat gcccggagcc ggttcgaata gtgacggcag agagacaatc aaatcatggc 421 tactgggaaa ggagccgccg ccagcaccca ggaaggcaaa agccaaccat ttaaagtgac 481 cccaggcccg ttcgatcctg ccacctggct ggaatggtca cgtcaatggc aaggtacgga 541 gggcaacggc catgcggcag cgtccggcat ccctgggctt gatgcactgg ctggtgttaa 601 aattgcgcct gcccaattgg gagacatcca acagcgttat atgaaagatt tttcggcgct 661 gtggcaggcg atggcggaag gtaaagcaga ggcgacggga ccgctgcatg accgtcgctt 721 tgctggcgac gcctggcgta ccaatttgcc ctatcgtttt gcggcagcct tctacctgct 781 gaacgctcgt gccctgactg agctggcaga tgctgtcgaa gcggatgcca aaacccgcca 841 acgcatccgt tttgccatct cacagtgggt cgacgcaatg tcaccggcca acttcctggc 901 caccaatccg gaagcacagc gcctcctgat tgaaagcggc ggggaatcgc tgcgcgccgg 961 cgttcgtaac atgatggaag atctgacccg tggtaagatt tcgcagacgg atgagtcggc 1021 cttcgaggtg ggtcgcaatg ttgcagtgac agaaggcgcg gtggtgtttg agaacgaata 1081 ctttcaactt ctccagtata aaccactgac ggataaagtg catgcgcgcc cattgttaat 1141 ggttcccccg tgcattaata aatactatat cctggatctt cagccggaaa gctccttagt 1201 gcgccacgtg gtggaacagg ggcatactgt gtttttagtt agctggcgca atccagatgc 1261 ctcaatggcc ggcagtacct gggatgacta cattgaacac gcggctattc gcgcgatcga 1321 agtggcacgc gacatctcag gtcaggacaa gattaacgta ctcggctttt gcgtcggtgg 1381 cactatcgtg agcaccgctc tggcggtact ggctgcgcgt ggtgaacacc ccgcagcgtc 1441 ggttacgctt ctgaccacac tgttggattt cgccgacact ggcatcttgg atgtatttgt 1501 cgatgagggc cacgttcagc tgcgcgaggc cactctgggt ggcggcgccg gcgcaccgtg 1561 cgctctgctg cgcggtctgg agctcgcgaa tactttttct ttcttacgcc cgaacgatct 1621 tgtctggaat tacgtagttg acaactatct caaaggtaac acgcccgtgc cgtttgattt 1681 attattctgg aatggggatg cgaccaacct gccgggtcct tggtattgtt ggtatctccg 1741 tcatacatac ttacagaatg aattaaaggt cccaggtaaa cttactgtgt gtggtgtccc 1801 ggtagatctg gcgagtatcg acgtgccgac ctacatttat gggagtcgtg aagatcatat 1861 tgtgccgtgg accgccgcgt acgcttctac cgcgcttctg gcaaacaaat tacgtttcgt 1921 tctgggtgcc agcggccaca ttgcgggtgt tattaatcct ccggccaaaa ataaacgctc 1981 gcattggaca aacgacgcct tgccggagag ccctcagcag tggttggcgg gtgccattga 2041 gcaccatgga agttggtggc cggactggac agcctggctg gcgggccaag caggcgcaaa 2101 acgcgctgcg ccagccaact atggcaacgc gcgctaccgc gcgattgaac ccgcgcctgg 2161 ccgctatgta aaagcaaaag cgtgacgctt gcatgagtgc cggcgtgcgt catgcacggc 2221 gccggcaggc ctgcacgttc cctcccgttt ccattgaaag gactacacaa tgaccgatgt 2281 tgttatcgtc agtgccgcgc gcaccgccgt gggtaaattt ggcggcagtc tggcgaaaat 2341 cccggcgcca gaattgggcg cggtggtaat caaagcggcc ctggaacgcg ccggtgtgaa 2401 acctgagcag gtgagcgaag taattatggg tcaggttctg accgcgggca gcgggcaaaa 2461 tccggcgcgt caggctgcga ttaaggcagg ccttccggcg atggtgcccg cgatgaccat 2521 taacaaagta tgcggatcgg gactgaaagc agtgatgctg gccgctaacg ccattatggc 2581 aggcgacgcg gaaattgtgg tcgctggcgg acaggagaat atgtcagccg cgcctcatgt 2641 gctgccgggt agccgcgacg gctttcgcat gggtgatgca aaactggtcg atactatgat 2701 tgttgacggc ttatgggatg tgtacaacca gtaccacatg ggcatcaccg ccgagaacgt 2761 ggccaaggaa tacggcatca cacgcgaggc gcaggatgag ttcgccgtcg gctcgcagaa 2821 caaggccgaa gccgcgcaga aggccggcat gtttgacgaa gagatcgtcc cggtgctgat 2881 cccgcagcgc aagggcgacc cggtggcctt caagaccgac gagttcgtgc gccagggcgc 2941 cacgctggac agcatgtccg gcctcaagcc cgccttcgac aaggccggca cggtgaccgc 3001 ggccaacgcc tcgggcctga acgacggcgc cgccgcggtg gtggtgatgt cggcggccaa 3061 ggccaaggaa ctgggcctga ccccgctggc cacgatcaag agctatgcca acgccggtgt 3121 cgatcccaag gtgatgggca tgggcccggt gccggcctcc aagcgcgccc tgtcgcgcgc 3181 cgagtggacc ccgcaagacc tggacctgat ggagatcaac gaggcctttg ccgcgcaggc 3241 gctggcggtg caccagcaga tgggctggga cacctccaag gtcaatgtga acggcggcgc 3301 catcgccatc ggccacccga tcggcgcgtc gggctgccgt atcctggtga cgctgctgca 3361 cgagatgaag cgccgtgacg cgaagaaggg cctggcctcg ctgtgcatcg gcggcggcat 3421 gggcgtggcg ctggcagtcg agcgcaaata aggaaggggt tttccggggc cgcgcgcggt 3481 tggcgcggac ccggcgacga taacgaagcc aatcaaggag tggacatgac tcagcgcatt 3541 gcgtatgtga ccggcggcat gggtggtatc ggaaccgcca tttgccagcg gctggccaag 3601 gatggctttc gtgtggtggc cggttgcggc cccaactcgc cgcgccgcga aaagtggctg 3661 gagcagcaga aggccctggg cttcgatttc attgcctcgg aaggcaatgt ggctgactgg 3721 gactcgacca agaccgcatt cgacaaggtc aagtccgagg tcggcgaggt tgatgtgctg 3781 atcaacaacg ccggtatcac ccgcgacgtg gtgttccgca agatgacccg cgccgactgg 3841 gatgcggtga tcgacaccaa cctgacctcg ctgttcaacg tcaccaagca ggtgatcgac 3901 ggcatggccg accgtggctg gggccgcatc gtcaacatct cgtcggtgaa cgggcagaag 3961 ggccagttcg gccagaccaa ctactccacc gccaaggccg gcctgcatgg cttcaccatg 4021 gcactggcgc aggaagtggc gaccaagggc gtgaccgtca acacggtctc tccgggctat 4081 atcgccaccg acatggtcaa ggcgatccgc caggacgtgc tcgacaagat cgtcgcgacg 4141 atcccggtca agcgcctggg cctgccggaa gagatcgcct cgatctgcgc ctggttgtcg 4201 tcggaggagt ccggtttctc gaccggcgcc gacttctcgc tcaacggcgg cctgcatatg 4261 ggctgacctg c //