BBa_K934001 1 phaC1-A-B1 phaC1-A-B1 [P(3HB) synthesis] 2012-09-16T11:00:00Z 2015-05-08T01:13:47Z test Released HQ 2013 The sequence comes from Ralstonia eutropha H16 adapted to become a standard BioBrick. false false _1199_ 0 14016 9 In stock true test false Taku Nakayama annotation2192330 1 RBS range2192330 1 2196 2199 annotation2192327 1 phaB1 range2192327 1 3463 4203 annotation2192325 1 phaC1 range2192325 1 353 2122 annotation2192331 1 RBS range2192331 1 3453 3456 annotation2192326 1 phaA range2192326 1 2207 3388 annotation2192329 1 RBS range2192329 1 336 342 annotation2192328 1 Pwt range2192328 1 1 50 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23104 1 BBa_J23104 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z isolated from library of promoters replace later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_K2018000 1 BBa_K2018000 phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB. 2016-05-19T11:00:00Z 2016-05-20T06:52:33Z Bla bla bla Bla bla bla false false _2485_ 30977 30977 9 false This part is created as part of an experiment to improve the phaCAB biobrick and understand how additional promoter and RBS sites improve yield. false Jakob R??nning & Joel component2479705 1 BBa_K608002 component2479713 1 BBa_K934001 annotation2479705 1 BBa_K608002 range2479705 1 1 55 annotation2479713 1 BBa_K934001 range2479713 1 64 4271 BBa_K608002 1 BBa_K608002 strong Promoter and strong RBS 2011-09-14T11:00:00Z 2015-05-08T01:12:52Z assembly from iGEM parts Released HQ 2013 you can insert your part behind this part so it will be immediatly expressed in e.coli. false false _780_ 0 9115 9 In stock false cloning via 3a-assembly false Julia M??ller component2128648 1 BBa_B0034 component2128646 1 BBa_J23104 annotation2128646 1 BBa_J23104 range2128646 1 1 35 annotation2128648 1 BBa_B0034 range2128648 1 44 55 BBa_K934001_sequence 1 gattcccgcattgacagcgcgtgcgttgcaaggcaacaatggactcaaatgtctcggaatcgctgacgattcccaggtttctccggcaagcatagcgcatggcgtctccatgcgagaatgtcgcgcttgccggataaaaggggagccgctatcggaatggacgcaagccacggccgcagcaggtgcggtcgagggcttccagccagttccagggcagatgtgccggcagaccctcccgctttgggggaggcgcaagccgggtccattcggatagcatctccccatgcaaagtgccggccagggcaatgcccggagccggttcgaatagtgacggcagagagacaatcaaatcatggctactgggaaaggagccgccgccagcacccaggaaggcaaaagccaaccatttaaagtgaccccaggcccgttcgatcctgccacctggctggaatggtcacgtcaatggcaaggtacggagggcaacggccatgcggcagcgtccggcatccctgggcttgatgcactggctggtgttaaaattgcgcctgcccaattgggagacatccaacagcgttatatgaaagatttttcggcgctgtggcaggcgatggcggaaggtaaagcagaggcgacgggaccgctgcatgaccgtcgctttgctggcgacgcctggcgtaccaatttgccctatcgttttgcggcagccttctacctgctgaacgctcgtgccctgactgagctggcagatgctgtcgaagcggatgccaaaacccgccaacgcatccgttttgccatctcacagtgggtcgacgcaatgtcaccggccaacttcctggccaccaatccggaagcacagcgcctcctgattgaaagcggcggggaatcgctgcgcgccggcgttcgtaacatgatggaagatctgacccgtggtaagatttcgcagacggatgagtcggccttcgaggtgggtcgcaatgttgcagtgacagaaggcgcggtggtgtttgagaacgaatactttcaacttctccagtataaaccactgacggataaagtgcatgcgcgcccattgttaatggttcccccgtgcattaataaatactatatcctggatcttcagccggaaagctccttagtgcgccacgtggtggaacaggggcatactgtgtttttagttagctggcgcaatccagatgcctcaatggccggcagtacctgggatgactacattgaacacgcggctattcgcgcgatcgaagtggcacgcgacatctcaggtcaggacaagattaacgtactcggcttttgcgtcggtggcactatcgtgagcaccgctctggcggtactggctgcgcgtggtgaacaccccgcagcgtcggttacgcttctgaccacactgttggatttcgccgacactggcatcttggatgtatttgtcgatgagggccacgttcagctgcgcgaggccactctgggtggcggcgccggcgcaccgtgcgctctgctgcgcggtctggagctcgcgaatactttttctttcttacgcccgaacgatcttgtctggaattacgtagttgacaactatctcaaaggtaacacgcccgtgccgtttgatttattattctggaatggggatgcgaccaacctgccgggtccttggtattgttggtatctccgtcatacatacttacagaatgaattaaaggtcccaggtaaacttactgtgtgtggtgtcccggtagatctggcgagtatcgacgtgccgacctacatttatgggagtcgtgaagatcatattgtgccgtggaccgccgcgtacgcttctaccgcgcttctggcaaacaaattacgtttcgttctgggtgccagcggccacattgcgggtgttattaatcctccggccaaaaataaacgctcgcattggacaaacgacgccttgccggagagccctcagcagtggttggcgggtgccattgagcaccatggaagttggtggccggactggacagcctggctggcgggccaagcaggcgcaaaacgcgctgcgccagccaactatggcaacgcgcgctaccgcgcgattgaacccgcgcctggccgctatgtaaaagcaaaagcgtgacgcttgcatgagtgccggcgtgcgtcatgcacggcgccggcaggcctgcacgttccctcccgtttccattgaaaggactacacaatgaccgatgttgttatcgtcagtgccgcgcgcaccgccgtgggtaaatttggcggcagtctggcgaaaatcccggcgccagaattgggcgcggtggtaatcaaagcggccctggaacgcgccggtgtgaaacctgagcaggtgagcgaagtaattatgggtcaggttctgaccgcgggcagcgggcaaaatccggcgcgtcaggctgcgattaaggcaggccttccggcgatggtgcccgcgatgaccattaacaaagtatgcggatcgggactgaaagcagtgatgctggccgctaacgccattatggcaggcgacgcggaaattgtggtcgctggcggacaggagaatatgtcagccgcgcctcatgtgctgccgggtagccgcgacggctttcgcatgggtgatgcaaaactggtcgatactatgattgttgacggcttatgggatgtgtacaaccagtaccacatgggcatcaccgccgagaacgtggccaaggaatacggcatcacacgcgaggcgcaggatgagttcgccgtcggctcgcagaacaaggccgaagccgcgcagaaggccggcatgtttgacgaagagatcgtcccggtgctgatcccgcagcgcaagggcgacccggtggccttcaagaccgacgagttcgtgcgccagggcgccacgctggacagcatgtccggcctcaagcccgccttcgacaaggccggcacggtgaccgcggccaacgcctcgggcctgaacgacggcgccgccgcggtggtggtgatgtcggcggccaaggccaaggaactgggcctgaccccgctggccacgatcaagagctatgccaacgccggtgtcgatcccaaggtgatgggcatgggcccggtgccggcctccaagcgcgccctgtcgcgcgccgagtggaccccgcaagacctggacctgatggagatcaacgaggcctttgccgcgcaggcgctggcggtgcaccagcagatgggctgggacacctccaaggtcaatgtgaacggcggcgccatcgccatcggccacccgatcggcgcgtcgggctgccgtatcctggtgacgctgctgcacgagatgaagcgccgtgacgcgaagaagggcctggcctcgctgtgcatcggcggcggcatgggcgtggcgctggcagtcgagcgcaaataaggaaggggttttccggggccgcgcgcggttggcgcggacccggcgacgataacgaagccaatcaaggagtggacatgactcagcgcattgcgtatgtgaccggcggcatgggtggtatcggaaccgccatttgccagcggctggccaaggatggctttcgtgtggtggccggttgcggccccaactcgccgcgccgcgaaaagtggctggagcagcagaaggccctgggcttcgatttcattgcctcggaaggcaatgtggctgactgggactcgaccaagaccgcattcgacaaggtcaagtccgaggtcggcgaggttgatgtgctgatcaacaacgccggtatcacccgcgacgtggtgttccgcaagatgacccgcgccgactgggatgcggtgatcgacaccaacctgacctcgctgttcaacgtcaccaagcaggtgatcgacggcatggccgaccgtggctggggccgcatcgtcaacatctcgtcggtgaacgggcagaagggccagttcggccagaccaactactccaccgccaaggccggcctgcatggcttcaccatggcactggcgcaggaagtggcgaccaagggcgtgaccgtcaacacggtctctccgggctatatcgccaccgacatggtcaaggcgatccgccaggacgtgctcgacaagatcgtcgcgacgatcccggtcaagcgcctgggcctgccggaagagatcgcctcgatctgcgcctggttgtcgtcggaggagtccggtttctcgaccggcgccgacttctcgctcaacggcggcctgcatatgggctgacctgc BBa_K2018000_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagaggattcccgcattgacagcgcgtgcgttgcaaggcaacaatggactcaaatgtctcggaatcgctgacgattcccaggtttctccggcaagcatagcgcatggcgtctccatgcgagaatgtcgcgcttgccggataaaaggggagccgctatcggaatggacgcaagccacggccgcagcaggtgcggtcgagggcttccagccagttccagggcagatgtgccggcagaccctcccgctttgggggaggcgcaagccgggtccattcggatagcatctccccatgcaaagtgccggccagggcaatgcccggagccggttcgaatagtgacggcagagagacaatcaaatcatggctactgggaaaggagccgccgccagcacccaggaaggcaaaagccaaccatttaaagtgaccccaggcccgttcgatcctgccacctggctggaatggtcacgtcaatggcaaggtacggagggcaacggccatgcggcagcgtccggcatccctgggcttgatgcactggctggtgttaaaattgcgcctgcccaattgggagacatccaacagcgttatatgaaagatttttcggcgctgtggcaggcgatggcggaaggtaaagcagaggcgacgggaccgctgcatgaccgtcgctttgctggcgacgcctggcgtaccaatttgccctatcgttttgcggcagccttctacctgctgaacgctcgtgccctgactgagctggcagatgctgtcgaagcggatgccaaaacccgccaacgcatccgttttgccatctcacagtgggtcgacgcaatgtcaccggccaacttcctggccaccaatccggaagcacagcgcctcctgattgaaagcggcggggaatcgctgcgcgccggcgttcgtaacatgatggaagatctgacccgtggtaagatttcgcagacggatgagtcggccttcgaggtgggtcgcaatgttgcagtgacagaaggcgcggtggtgtttgagaacgaatactttcaacttctccagtataaaccactgacggataaagtgcatgcgcgcccattgttaatggttcccccgtgcattaataaatactatatcctggatcttcagccggaaagctccttagtgcgccacgtggtggaacaggggcatactgtgtttttagttagctggcgcaatccagatgcctcaatggccggcagtacctgggatgactacattgaacacgcggctattcgcgcgatcgaagtggcacgcgacatctcaggtcaggacaagattaacgtactcggcttttgcgtcggtggcactatcgtgagcaccgctctggcggtactggctgcgcgtggtgaacaccccgcagcgtcggttacgcttctgaccacactgttggatttcgccgacactggcatcttggatgtatttgtcgatgagggccacgttcagctgcgcgaggccactctgggtggcggcgccggcgcaccgtgcgctctgctgcgcggtctggagctcgcgaatactttttctttcttacgcccgaacgatcttgtctggaattacgtagttgacaactatctcaaaggtaacacgcccgtgccgtttgatttattattctggaatggggatgcgaccaacctgccgggtccttggtattgttggtatctccgtcatacatacttacagaatgaattaaaggtcccaggtaaacttactgtgtgtggtgtcccggtagatctggcgagtatcgacgtgccgacctacatttatgggagtcgtgaagatcatattgtgccgtggaccgccgcgtacgcttctaccgcgcttctggcaaacaaattacgtttcgttctgggtgccagcggccacattgcgggtgttattaatcctccggccaaaaataaacgctcgcattggacaaacgacgccttgccggagagccctcagcagtggttggcgggtgccattgagcaccatggaagttggtggccggactggacagcctggctggcgggccaagcaggcgcaaaacgcgctgcgccagccaactatggcaacgcgcgctaccgcgcgattgaacccgcgcctggccgctatgtaaaagcaaaagcgtgacgcttgcatgagtgccggcgtgcgtcatgcacggcgccggcaggcctgcacgttccctcccgtttccattgaaaggactacacaatgaccgatgttgttatcgtcagtgccgcgcgcaccgccgtgggtaaatttggcggcagtctggcgaaaatcccggcgccagaattgggcgcggtggtaatcaaagcggccctggaacgcgccggtgtgaaacctgagcaggtgagcgaagtaattatgggtcaggttctgaccgcgggcagcgggcaaaatccggcgcgtcaggctgcgattaaggcaggccttccggcgatggtgcccgcgatgaccattaacaaagtatgcggatcgggactgaaagcagtgatgctggccgctaacgccattatggcaggcgacgcggaaattgtggtcgctggcggacaggagaatatgtcagccgcgcctcatgtgctgccgggtagccgcgacggctttcgcatgggtgatgcaaaactggtcgatactatgattgttgacggcttatgggatgtgtacaaccagtaccacatgggcatcaccgccgagaacgtggccaaggaatacggcatcacacgcgaggcgcaggatgagttcgccgtcggctcgcagaacaaggccgaagccgcgcagaaggccggcatgtttgacgaagagatcgtcccggtgctgatcccgcagcgcaagggcgacccggtggccttcaagaccgacgagttcgtgcgccagggcgccacgctggacagcatgtccggcctcaagcccgccttcgacaaggccggcacggtgaccgcggccaacgcctcgggcctgaacgacggcgccgccgcggtggtggtgatgtcggcggccaaggccaaggaactgggcctgaccccgctggccacgatcaagagctatgccaacgccggtgtcgatcccaaggtgatgggcatgggcccggtgccggcctccaagcgcgccctgtcgcgcgccgagtggaccccgcaagacctggacctgatggagatcaacgaggcctttgccgcgcaggcgctggcggtgcaccagcagatgggctgggacacctccaaggtcaatgtgaacggcggcgccatcgccatcggccacccgatcggcgcgtcgggctgccgtatcctggtgacgctgctgcacgagatgaagcgccgtgacgcgaagaagggcctggcctcgctgtgcatcggcggcggcatgggcgtggcgctggcagtcgagcgcaaataaggaaggggttttccggggccgcgcgcggttggcgcggacccggcgacgataacgaagccaatcaaggagtggacatgactcagcgcattgcgtatgtgaccggcggcatgggtggtatcggaaccgccatttgccagcggctggccaaggatggctttcgtgtggtggccggttgcggccccaactcgccgcgccgcgaaaagtggctggagcagcagaaggccctgggcttcgatttcattgcctcggaaggcaatgtggctgactgggactcgaccaagaccgcattcgacaaggtcaagtccgaggtcggcgaggttgatgtgctgatcaacaacgccggtatcacccgcgacgtggtgttccgcaagatgacccgcgccgactgggatgcggtgatcgacaccaacctgacctcgctgttcaacgtcaccaagcaggtgatcgacggcatggccgaccgtggctggggccgcatcgtcaacatctcgtcggtgaacgggcagaagggccagttcggccagaccaactactccaccgccaaggccggcctgcatggcttcaccatggcactggcgcaggaagtggcgaccaagggcgtgaccgtcaacacggtctctccgggctatatcgccaccgacatggtcaaggcgatccgccaggacgtgctcgacaagatcgtcgcgacgatcccggtcaagcgcctgggcctgccggaagagatcgcctcgatctgcgcctggttgtcgtcggaggagtccggtttctcgaccggcgccgacttctcgctcaacggcggcctgcatatgggctgacctgc BBa_B0034_sequence 1 aaagaggagaaa BBa_K608002_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa BBa_J23104_sequence 1 ttgacagctagctcagtcctaggtattgtgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z