BBa_K2018017
1
BBa_K2018017
Lysostaphin
2016-06-06T11:00:00Z
2016-06-07T01:35:16Z
..
..
true
false
_2485_
30819
30819
9
false
..
false
Brian Kenn Baltzar
component2480004
1
BBa_K608002
component2480005
1
BBa_K748002
annotation2480005
1
BBa_K748002
range2480005
1
62
802
annotation2480004
1
BBa_K608002
range2480004
1
1
55
BBa_K608002
1
BBa_K608002
strong Promoter and strong RBS
2011-09-14T11:00:00Z
2015-05-08T01:12:52Z
assembly from iGEM parts
Released HQ 2013
you can insert your part behind this part so it will be immediatly expressed in e.coli.
false
false
_780_
0
9115
9
In stock
false
cloning via 3a-assembly
false
Julia M??ller
component2128648
1
BBa_B0034
component2128646
1
BBa_J23104
annotation2128646
1
BBa_J23104
range2128646
1
1
35
annotation2128648
1
BBa_B0034
range2128648
1
44
55
BBa_K748002
1
BBa_K748002
Truncated lysostaphin coding sequence. Lysostaphin has has a specific lytic action against S.aureus.
2012-09-14T11:00:00Z
2015-05-08T01:13:12Z
de novo synthesis
Released HQ 2013
Lysostaphin is a zinc metalloenzyme that has a specific lytic action against S.aureus. Lysostaphin has activities of three enzymes namely, endo-β-N-acetyl glucosamidase, N-acteyl-muramyl-L-alanine amidase, glycylglycine endopeptidase. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the poly-glycine bridges which form cross links between glycopeptide chains in cell wall peptidoglycan of S.aureus cells. The wild-type lysostaphin gene encodes a preproenzyme, and the conversion of prolysostaphin to mature lysostaphin occurs extracellularly and involves the removal of the hydrophilic tandem repeat portion of the proenzyme. In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence.
false
false
_999_
0
8564
9
In stock
false
In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence.
false
Lei Qiao
BBa_J23104
1
BBa_J23104
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
isolated from library of promoters
replace later
false
false
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K608002_sequence
1
ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa
BBa_K2018017_sequence
1
ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgacacatgaacattcagcacaatggttgaataattacaaaaaaggatatggttacggtccttatccattaggtataaatggcggtatgcactacggagttgatttttttatgaatattggaacaccagtaaaagctatttcaagcggaaaaatagttgaagctggttggagtaattacggaggaggtaatcaaataggtcttattgaaaatgatggagtgcatagacaatggtatatgcatctaagtaaatataatgttaaagtaggagattatgtcaaagctggtcaaataatcggttggtctggaagcactggttattctacagcaccacatttacacttccaaagaatggttaattcattttcaaattcaactgcccaagatccaatgcctttcttaaagagcgcaggatatggaaaagcaggtggtacagtaactccaacgccgaatacaggttggaaaacaaacaaatatggcacactatataaatcagagtcagctagcttcacacctaatacagatataataacaagaacgactggtccatttagaagcatgccgcagtcaggagtcttaaaagcaggtcaaacaattcattatgatgaagtgatgaaacaagacggtcatgtttgggtaggttatacaggtaacagtggccaacgtatttacttgcctgtaagaacatggaataaatctactaatactttaggtgttctttggggaactataaagtaataa
BBa_J23104_sequence
1
ttgacagctagctcagtcctaggtattgtgctagc
BBa_K748002_sequence
1
atgacacatgaacattcagcacaatggttgaataattacaaaaaaggatatggttacggtccttatccattaggtataaatggcggtatgcactacggagttgatttttttatgaatattggaacaccagtaaaagctatttcaagcggaaaaatagttgaagctggttggagtaattacggaggaggtaatcaaataggtcttattgaaaatgatggagtgcatagacaatggtatatgcatctaagtaaatataatgttaaagtaggagattatgtcaaagctggtcaaataatcggttggtctggaagcactggttattctacagcaccacatttacacttccaaagaatggttaattcattttcaaattcaactgcccaagatccaatgcctttcttaaagagcgcaggatatggaaaagcaggtggtacagtaactccaacgccgaatacaggttggaaaacaaacaaatatggcacactatataaatcagagtcagctagcttcacacctaatacagatataataacaagaacgactggtccatttagaagcatgccgcagtcaggagtcttaaaagcaggtcaaacaattcattatgatgaagtgatgaaacaagacggtcatgtttgggtaggttatacaggtaacagtggccaacgtatttacttgcctgtaagaacatggaataaatctactaatactttaggtgttctttggggaactataaagtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z