LOCUS       BBa_K2018019            1696 bp    DNA     linear       12-May-2024
DEFINITION  Pyocin S5
ACCESSION   BBa_K2018019
VERSION     BBa_K2018019.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..1559
                     /label=BBa_K2018018
     stem_loop       1579..1622
                     /label=stem_loop
     misc_feature    1568..1647
                     /label=BBa_B0010
     terminator      1568..1647
                     /label=BBa_B0010
     stem_loop       1663..1682
                     /label=T7 TE
     misc_feature    1689..1689
                     /label=stop
     polyA_site      1683..1696
                     /label=polya
     misc_feature    1656..1696
                     /label=BBa_B0012
     terminator      1656..1696
                     /label=BBa_B0012
     terminator      1568..1696
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagtcacaca ggaaagtact
       61 agatgtcaaa cgacaacgaa gtcccgggga gcatggtgat cgtggctcag ggacctgatg
      121 accaatacgc ttacgaagtt ccgcccattg acagtgctgc ggtagctgga aatatgttcg
      181 gtgacttgat ccagcgtgac atttatctgc agaaaaacat ttactaccct gtccgttcaa
      241 tttttgagca aggcacgaag gaaaagaaag aaatcaacaa gaaggttagc gatcaagtcg
      301 acggcctttt gaaacaaatt acacaaggca aacgcgaagc gacgcgccaa gagcgcgtag
      361 atgttatgag cgctgttctt cacaaaatgg agtcggacct tgaagggtac aaaaagactt
      421 ttacaaaagg gccctttatc gattatgaga aacagtcttc cttatcaatc tacgaggctt
      481 gggtcaagat ttgggaaaaa aacagttggg aggagcgcaa gaagtatccc ttccagcaat
      541 tagtccgcga cgagctggaa cgtgcagtgg cttactataa gcaagattcg ctgtcggagg
      601 ctgtcaaggt acttcgtcag gaactgaaca aacagaaggc gttaaaagag aaggaagatt
      661 tgtcgcaatt ggagcgtgat tatcgtaccc gcaaggccaa tttggagatg aaagtacaga
      721 gcgaacttga ccaagcaggc agcgccttgc cccctcttgt atccccaacc cccgaacagt
      781 ggctggaacg tgctactcgc cttgtcactc aggcgattgc cgataaaaag caactgcaaa
      841 ctacgaataa cacgttgatc aaaaatagcc caaccccgtt ggagaagcaa aaggcaatct
      901 acaacggaga gttgcttgtt gacgagattg cgtcgcttca ggcccgctta gttaagttaa
      961 atgcagaaac tacccgtcgc cgcacggaag cagagcgcaa agaagccgag gagcaggccc
     1021 tgcaggacgc catcaaattt acagccgact tctataaaga agtgacagaa aaattcgggg
     1081 ctcgcacctc ggagatggct cgtcaactgg ccgaaggcgc ccgcggtaag aatatccgtt
     1141 catcggcgga ggcaattaag tccttcgaga aacacaaaga tgcactgaac aagaagttgt
     1201 cattaaagga tcgccaggcc atcgccaagg cgtttgattc cttagacaaa cagatgatgg
     1261 caaagtcctt agagaagttt tctaagggct ttggggtggt gggtaaagcg atcgatgcgg
     1321 catccttgta tcaggaattt aaaatcagca ccgagaccgg ggactggaag ccattcttcg
     1381 tgaagattga aacgttagcg gccggagccg ccgcgtcttg gctggtaggc atcgctttcg
     1441 ccacagcaac ggctacgcca attggtatct taggatttgc tctggtaatg gccgtaacag
     1501 gcgctatgat cgacgaggat cttcttgaga aagcgaataa tctggttatt tctatttaat
     1561 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1621 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1681 cctttctgcg tttata
//