BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_J23104 1 BBa_J23104 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z isolated from library of promoters replace later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_K2018018 1 BBa_K2018018 Pyocin S5 2016-06-08T11:00:00Z 2016-06-09T02:46:57Z . . false false _2485_ 30819 30819 9 false . false Brian Kenn Baltzar BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K2018019 1 BBa_K2018019 Pyocin S5 2016-06-08T11:00:00Z 2016-06-09T02:32:31Z . . false false _2485_ 30819 30819 9 false . false Brian Kenn Baltzar component2480091 1 BBa_K2018018 component2480098 1 BBa_B0015 component2480087 1 BBa_J23104 component2480089 1 BBa_B0032 annotation2480089 1 BBa_B0032 range2480089 1 44 56 annotation2480091 1 BBa_K2018018 range2480091 1 63 1559 annotation2480098 1 BBa_B0015 range2480098 1 1568 1696 annotation2480087 1 BBa_J23104 range2480087 1 1 35 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2018019_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaagtactagatgtcaaacgacaacgaagtcccggggagcatggtgatcgtggctcagggacctgatgaccaatacgcttacgaagttccgcccattgacagtgctgcggtagctggaaatatgttcggtgacttgatccagcgtgacatttatctgcagaaaaacatttactaccctgtccgttcaatttttgagcaaggcacgaaggaaaagaaagaaatcaacaagaaggttagcgatcaagtcgacggccttttgaaacaaattacacaaggcaaacgcgaagcgacgcgccaagagcgcgtagatgttatgagcgctgttcttcacaaaatggagtcggaccttgaagggtacaaaaagacttttacaaaagggccctttatcgattatgagaaacagtcttccttatcaatctacgaggcttgggtcaagatttgggaaaaaaacagttgggaggagcgcaagaagtatcccttccagcaattagtccgcgacgagctggaacgtgcagtggcttactataagcaagattcgctgtcggaggctgtcaaggtacttcgtcaggaactgaacaaacagaaggcgttaaaagagaaggaagatttgtcgcaattggagcgtgattatcgtacccgcaaggccaatttggagatgaaagtacagagcgaacttgaccaagcaggcagcgccttgccccctcttgtatccccaacccccgaacagtggctggaacgtgctactcgccttgtcactcaggcgattgccgataaaaagcaactgcaaactacgaataacacgttgatcaaaaatagcccaaccccgttggagaagcaaaaggcaatctacaacggagagttgcttgttgacgagattgcgtcgcttcaggcccgcttagttaagttaaatgcagaaactacccgtcgccgcacggaagcagagcgcaaagaagccgaggagcaggccctgcaggacgccatcaaatttacagccgacttctataaagaagtgacagaaaaattcggggctcgcacctcggagatggctcgtcaactggccgaaggcgcccgcggtaagaatatccgttcatcggcggaggcaattaagtccttcgagaaacacaaagatgcactgaacaagaagttgtcattaaaggatcgccaggccatcgccaaggcgtttgattccttagacaaacagatgatggcaaagtccttagagaagttttctaagggctttggggtggtgggtaaagcgatcgatgcggcatccttgtatcaggaatttaaaatcagcaccgagaccggggactggaagccattcttcgtgaagattgaaacgttagcggccggagccgccgcgtcttggctggtaggcatcgctttcgccacagcaacggctacgccaattggtatcttaggatttgctctggtaatggccgtaacaggcgctatgatcgacgaggatcttcttgagaaagcgaataatctggttatttctatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0032_sequence 1 tcacacaggaaag BBa_K2018018_sequence 1 atgtcaaacgacaacgaagtcccggggagcatggtgatcgtggctcagggacctgatgaccaatacgcttacgaagttccgcccattgacagtgctgcggtagctggaaatatgttcggtgacttgatccagcgtgacatttatctgcaaaaaaacatttactaccctgtccgttcaatttttgagcaaggcacgaaggaaaagaaagaaatcaacaagaaggttagcgatcaagtcgacggccttttgaaacaaattacacaaggcaaacgcgaagcgacgcgccaagagcgcgtagatgttatgagcgctgttcttcacaaaatggagtcggaccttgaagggtacaaaaagacttttacaaaagggccctttatcgattatgagaaacagtcttccttatcaatctacgaggcttgggtcaagatttgggaaaaaaacagttgggaggagcgcaagaagtatcccttccagcaattagtccgcgacgagctggaacgtgcagtggcttactataagcaagattcgctgtcggaggctgtcaaggtacttcgtcaggaactgaacaaacagaaggcgttaaaagagaaggaagatttgtcgcaattggagcgtgattatcgtacccgcaaggccaatttggagatgaaagtacagagcgaacttgaccaagcaggcagcgccttgccccctcttgtatccccaacccccgaacagtggctggaacgtgctactcgccttgtcactcaggcgattgccgataaaaagcaactgcaaactacgaataacacgttgatcaaaaatagcccaaccccgttggagaagcaaaaggcaatctacaacggagagttgcttgttgacgagattgcgtcgcttcaggcccgcttagttaagttaaatgcagaaactacccgtcgccgcacggaagcagagcgcaaagaagccgaggagcaggccctgcaagacgccatcaaatttacagccgacttctataaagaagtgacagaaaaattcggggctcgcacctcggagatggctcgtcaactggccgaaggcgcccgcggtaagaatatccgttcatcggcggaggcaattaagtccttcgagaaacacaaagatgcactgaacaagaagttgtcattaaaggatcgccaggccatcgccaaggcgtttgattccttagacaaacagatgatggcaaagtccttagagaagttttctaagggctttggggtggtgggtaaagcgatcgatgcggcatccttgtatcaggaatttaaaatcagcaccgagaccggggactggaagccattcttcgtgaagattgaaacgttagcggccggagccgccgcgtcttggctggtaggcatcgctttcgccacagcaacggctacgccaattggtatcttaggatttgctctggtaatggccgtaacaggcgctatgatcgacgaggatcttcttgagaaagcgaataatctggttatttctatttaa BBa_J23104_sequence 1 ttgacagctagctcagtcctaggtattgtgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z