##gff-version 3
##sequence-region BBa_K2018030 1 4809
BBa_K2018030	.	terminator	4681	4809	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2018030	.	terminator	4681	4760	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2018030	.	stem_loop	4692	4735	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2018030	.	engineered_region	4681	4760	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2018030	.	terminator	4769	4809	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2018030	.	engineered_region	4769	4809	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2018030	.	stop_codon	4802	4802	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2018030	.	stem_loop	4776	4795	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2018030	.	polyA_site	4796	4809	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2018030	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500
BBa_K2018030	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K2018030	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2018030	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K2018030	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K2018030	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K2018030	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K2018030	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K2018030	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K2018030	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K2018030	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K2018030	.	ribosome_entry_site	997	1067	.	+	0	ID=BBa_K2018026;Name=BBa_K2018026
BBa_K2018030	.	CDS	3233	4672	.	+	0	ID=BBa_K2018029;Name=BBa_K2018029
BBa_K2018030	.	ribosome_entry_site	3209	3226	.	+	0	ID=BBa_K2018028;Name=BBa_K2018028
BBa_K2018030	.	engineered_region	227	988	.	+	0	ID=BBa_K2018024;Name=BBa_K2018024
BBa_K2018030	.	tag	803	988	.	+	0	ID=BBa_K2018023;Name=BBa_K2018023;Parent=BBa_K2018024
BBa_K2018030	.	CDS	227	802	.	+	0	ID=BBa_K2018022;Name=BBa_K2018022;Parent=BBa_K2018024
BBa_K2018030	.	CDS	1074	3200	.	+	0	ID=BBa_K2018027;Name=BBa_K2018027
>BBa_K2018030
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgatcctcac
cccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtc
gaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgt
cggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacac
ccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcg
aagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagt
cggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaa
cttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaag
acgacggctgccttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagccg
caggtagcttcgatgttaaagaggaaagaactgccgcttctttattgcagttgtccggtaatgccagtgatttttcata
tggacggaactcaataaccctgaccacatcagcatagtaatactagagtatattaatttaaatgatagcaatcttactg
ggctgtgccacataagattgctattttttttggagtcatatactagatggattcttgtcataaaattgattatgggtta
tacgccctggagattttagcccaataccataacgtctctgttaacccggaagaaattaaacatagatttgatacagacg
ggacaggtctgggattaacgtcatggttgcttgctgcgaaatctttagaactaaaggtaaaacaggtaaaaaaaacaat
tgatcgattaaactttatttttctgcccgcattagtctggagagaggatggacgtcattttattctgactaaaatcagc
aaagaagtaaacagatatcttatttttgatttggagcagcgaaatccccgtgttctcgaacagtctgagtttgaggcgt
tatatcaggggcatattattcttattacttcccgttcttctgttaccgggaaactggcaaaatttgactttacctggtt
tattcctgccattataaaatacaggagaatatttattgaaacccttgttgtatctgtttttttacaattatttgcatta
ataaccccccttttttttcaggtggttatggacaaagtattagtgcacagggggttttcaacccttaatgttattactg
ttgcattatctgttgtagtggtgtttgagattatactcagcggtttaagaacttacatttttgcacatagtacaagtcg
gattgatgttgagttgggtgccaaactcttccggcatttactggcgctaccgatctcttattttgagagtcgtcgtgtt
ggtgatactgttgcgagggtaagagaattagaccagatccgtaattttctgacaggacaggcattaacatctgttttgg
acttattattttcactcatattttttgcggtaatgtggtattacagcccaaagcttactctggtgatcttattttcgct
gccttgttatgctgcatggtctgtttttattagccccattttgcgacgtcgccttgatgataagttttcacggaatgcg
gataatcaatctttcctggtggaatcagtaacggcgattaacactataaaagctatggcagtctcacctcagatgacga
acatatgggacaaacaattggcaggatatgttgccgcaggctttaaagtgacagtattagcaaccattggtcaacaagg
aatacagttaatacaaaagactgttatgatcatcaacctatggttgggagcacacctggttatttccggggatttaagt
attggtcagttaattgcttttaatatgcttgctggtcagattgttgcaccggttattcgccttgcacaaatctggcagg
atttccagcaggttggtatatcagttacccgccttggtgatgtgcttaactctccaactgaaagttatcatgggaaact
gacattgccggaaattaatggtgatatcacttttcgtaatatccggtttcgctataaacctgattctccggttattttg
gacaatatcaatcttagtattaagcagggggaggttattggtattgtcggacgttctggttcaggaaaaagcacattaa
ctaaattaattcaacgtttttatattcctgaaaatggccaggtattaattgatggacatgatcttgcgttggctgatcc
taactggttacgtcgtcaggtgggggttgtgttgcaggacaatgtgctgcttaatcgcagtattattgataatatttca
ctggctaatcctggcatgtccgtcgaaaaagttatttatgcagcgaaattagcaggcgctcatgattttatttctgatt
tgcgtgaggggtataacaccattgtcggggaacagggggcaggattatccggaggtcaacgtcaacgcatcgcaattgc
aagggcgctggtgaacaaccctaaaatactcatttttgatgaagcaaccagtgctctggattatgagtcggagcatgtc
atcatgcgcaatatgcacaaaatatgtaagggcagaacggttataatcattgctcatcgtctgtctacagtaaaaaatg
cagaccgcattattgtcatggaaaaagggaaaattgttgaacagggtaaacataaggagctgctttctgaaccggaaag
tttatacagttacttatatcagttacagtcagactaatagtactagagcagaaagaacagaagaattactagatgaaaa
catggttaatggggttcagcgagttcctgttgcgctataaacttgtctggagtgaaacatggaaaatccggaagcaatt
agatactccggtacgtgaaaaggacgaaaatgaatttttacccgctcatctggaattaattgaaacgccagtatccaga
cggccgcgtctggttgcttattttattatggggtttctggttattgcttttattttatctgttttaggccaagtggaaa
ttgttgccactgcaaatgggaaattaacacacagtgggcgtagtaaagaaattaaacctattgaaaactcaatagttaa
agaaattatcgtaaaagaaggagagtcagtccggaaaggggatgtgttattaaagcttacagcactgggagctgaagct
gatacgttaaaaacacagtcatcactgttacaggccaggctggaacaaactcggtatcaaattctgagcaggtcaattg
aattaaataaactacctgaactaaagcttcctgatgagccttattttcagaatgtatctgaagaggaagtactgcgttt
aacttctttgataaaagaacagttttccacatggcaaaatcagaagtatcaaaaagaactgaatttggataagaaaaga
gcagagcgattaacagtacttgcccgtataaaccgttatgaaaatttatcaagggttgaaaaaagccgtctggatgatt
tcagtagtttattgcataaacaggcaattgcaaaacatgctgtacttgagcaggagaataaatatgtcgaagcagtaaa
tgaattacgagtttataaatcacaactggagcaaattgagagtgagatattgtctgcaaaagaagaatatcagcttgtt
acgcagctttttaaaaatgaaattttagataagctaagacaaacaacagacaacattgggttattaactctggaattag
cgaaaaatgaagagcgtcaacaggcttcagtaatcagggccccagtttcgggaaaagttcagcaactgaaggttcatac
tgaaggtggggttgttacaacagcggaaacactgatggtcatcgttccggaagatgacacgctggaggttactgctctg
gtacaaaataaagatattggttttattaacgtcgggcagaatgccatcattaaagtggaggcatttccttatacacgat
atggttatctggtgggtaaggtgaaaaatataaatttagatgcaatagaagaccagagactgggacttgtttttaatgt
tattatttctattgaagagaattgtttgtcaaccgggaataaaaacattccattaagctcgggtatggcagtcactgcc
gaaataaagacaggtatgcgaagtgtaatcagttatcttcttagtcctttagaagagtcagtaacagaaagtttacgtg
agcgttagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata