LOCUS BBa_K2018034 4274 bp DNA linear 29-Nov-2024 DEFINITION phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB. ACCESSION BBa_K2018034 VERSION BBa_K2018034.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 misc_feature 1..58 /label=BBa_K081005 CDS 419..2188 /label=phaC1 CDS 2273..3454 /label=phaA CDS 3529..4269 /label=phaB1 promoter 67..116 /label=Pwt RBS 402..408 /label=RBS RBS 2262..2265 /label=RBS RBS 3519..3522 /label=RBS misc_feature 67..4274 /label=BBa_K934001 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata 61 ctagaggatt cccgcattga cagcgcgtgc gttgcaaggc aacaatggac tcaaatgtct 121 cggaatcgct gacgattccc aggtttctcc ggcaagcata gcgcatggcg tctccatgcg 181 agaatgtcgc gcttgccgga taaaagggga gccgctatcg gaatggacgc aagccacggc 241 cgcagcaggt gcggtcgagg gcttccagcc agttccaggg cagatgtgcc ggcagaccct 301 cccgctttgg gggaggcgca agccgggtcc attcggatag catctcccca tgcaaagtgc 361 cggccagggc aatgcccgga gccggttcga atagtgacgg cagagagaca atcaaatcat 421 ggctactggg aaaggagccg ccgccagcac ccaggaaggc aaaagccaac catttaaagt 481 gaccccaggc ccgttcgatc ctgccacctg gctggaatgg tcacgtcaat ggcaaggtac 541 ggagggcaac ggccatgcgg cagcgtccgg catccctggg cttgatgcac tggctggtgt 601 taaaattgcg cctgcccaat tgggagacat ccaacagcgt tatatgaaag atttttcggc 661 gctgtggcag gcgatggcgg aaggtaaagc agaggcgacg ggaccgctgc atgaccgtcg 721 ctttgctggc gacgcctggc gtaccaattt gccctatcgt tttgcggcag ccttctacct 781 gctgaacgct cgtgccctga ctgagctggc agatgctgtc gaagcggatg ccaaaacccg 841 ccaacgcatc cgttttgcca tctcacagtg ggtcgacgca atgtcaccgg ccaacttcct 901 ggccaccaat ccggaagcac agcgcctcct gattgaaagc ggcggggaat cgctgcgcgc 961 cggcgttcgt aacatgatgg aagatctgac ccgtggtaag atttcgcaga cggatgagtc 1021 ggccttcgag gtgggtcgca atgttgcagt gacagaaggc gcggtggtgt ttgagaacga 1081 atactttcaa cttctccagt ataaaccact gacggataaa gtgcatgcgc gcccattgtt 1141 aatggttccc ccgtgcatta ataaatacta tatcctggat cttcagccgg aaagctcctt 1201 agtgcgccac gtggtggaac aggggcatac tgtgttttta gttagctggc gcaatccaga 1261 tgcctcaatg gccggcagta cctgggatga ctacattgaa cacgcggcta ttcgcgcgat 1321 cgaagtggca cgcgacatct caggtcagga caagattaac gtactcggct tttgcgtcgg 1381 tggcactatc gtgagcaccg ctctggcggt actggctgcg cgtggtgaac accccgcagc 1441 gtcggttacg cttctgacca cactgttgga tttcgccgac actggcatct tggatgtatt 1501 tgtcgatgag ggccacgttc agctgcgcga ggccactctg ggtggcggcg ccggcgcacc 1561 gtgcgctctg ctgcgcggtc tggagctcgc gaatactttt tctttcttac gcccgaacga 1621 tcttgtctgg aattacgtag ttgacaacta tctcaaaggt aacacgcccg tgccgtttga 1681 tttattattc tggaatgggg atgcgaccaa cctgccgggt ccttggtatt gttggtatct 1741 ccgtcataca tacttacaga atgaattaaa ggtcccaggt aaacttactg tgtgtggtgt 1801 cccggtagat ctggcgagta tcgacgtgcc gacctacatt tatgggagtc gtgaagatca 1861 tattgtgccg tggaccgccg cgtacgcttc taccgcgctt ctggcaaaca aattacgttt 1921 cgttctgggt gccagcggcc acattgcggg tgttattaat cctccggcca aaaataaacg 1981 ctcgcattgg acaaacgacg ccttgccgga gagccctcag cagtggttgg cgggtgccat 2041 tgagcaccat ggaagttggt ggccggactg gacagcctgg ctggcgggcc aagcaggcgc 2101 aaaacgcgct gcgccagcca actatggcaa cgcgcgctac cgcgcgattg aacccgcgcc 2161 tggccgctat gtaaaagcaa aagcgtgacg cttgcatgag tgccggcgtg cgtcatgcac 2221 ggcgccggca ggcctgcacg ttccctcccg tttccattga aaggactaca caatgaccga 2281 tgttgttatc gtcagtgccg cgcgcaccgc cgtgggtaaa tttggcggca gtctggcgaa 2341 aatcccggcg ccagaattgg gcgcggtggt aatcaaagcg gccctggaac gcgccggtgt 2401 gaaacctgag caggtgagcg aagtaattat gggtcaggtt ctgaccgcgg gcagcgggca 2461 aaatccggcg cgtcaggctg cgattaaggc aggccttccg gcgatggtgc ccgcgatgac 2521 cattaacaaa gtatgcggat cgggactgaa agcagtgatg ctggccgcta acgccattat 2581 ggcaggcgac gcggaaattg tggtcgctgg cggacaggag aatatgtcag ccgcgcctca 2641 tgtgctgccg ggtagccgcg acggctttcg catgggtgat gcaaaactgg tcgatactat 2701 gattgttgac ggcttatggg atgtgtacaa ccagtaccac atgggcatca ccgccgagaa 2761 cgtggccaag gaatacggca tcacacgcga ggcgcaggat gagttcgccg tcggctcgca 2821 gaacaaggcc gaagccgcgc agaaggccgg catgtttgac gaagagatcg tcccggtgct 2881 gatcccgcag cgcaagggcg acccggtggc cttcaagacc gacgagttcg tgcgccaggg 2941 cgccacgctg gacagcatgt ccggcctcaa gcccgccttc gacaaggccg gcacggtgac 3001 cgcggccaac gcctcgggcc tgaacgacgg cgccgccgcg gtggtggtga tgtcggcggc 3061 caaggccaag gaactgggcc tgaccccgct ggccacgatc aagagctatg ccaacgccgg 3121 tgtcgatccc aaggtgatgg gcatgggccc ggtgccggcc tccaagcgcg ccctgtcgcg 3181 cgccgagtgg accccgcaag acctggacct gatggagatc aacgaggcct ttgccgcgca 3241 ggcgctggcg gtgcaccagc agatgggctg ggacacctcc aaggtcaatg tgaacggcgg 3301 cgccatcgcc atcggccacc cgatcggcgc gtcgggctgc cgtatcctgg tgacgctgct 3361 gcacgagatg aagcgccgtg acgcgaagaa gggcctggcc tcgctgtgca tcggcggcgg 3421 catgggcgtg gcgctggcag tcgagcgcaa ataaggaagg ggttttccgg ggccgcgcgc 3481 ggttggcgcg gacccggcga cgataacgaa gccaatcaag gagtggacat gactcagcgc 3541 attgcgtatg tgaccggcgg catgggtggt atcggaaccg ccatttgcca gcggctggcc 3601 aaggatggct ttcgtgtggt ggccggttgc ggccccaact cgccgcgccg cgaaaagtgg 3661 ctggagcagc agaaggccct gggcttcgat ttcattgcct cggaaggcaa tgtggctgac 3721 tgggactcga ccaagaccgc attcgacaag gtcaagtccg aggtcggcga ggttgatgtg 3781 ctgatcaaca acgccggtat cacccgcgac gtggtgttcc gcaagatgac ccgcgccgac 3841 tgggatgcgg tgatcgacac caacctgacc tcgctgttca acgtcaccaa gcaggtgatc 3901 gacggcatgg ccgaccgtgg ctggggccgc atcgtcaaca tctcgtcggt gaacgggcag 3961 aagggccagt tcggccagac caactactcc accgccaagg ccggcctgca tggcttcacc 4021 atggcactgg cgcaggaagt ggcgaccaag ggcgtgaccg tcaacacggt ctctccgggc 4081 tatatcgcca ccgacatggt caaggcgatc cgccaggacg tgctcgacaa gatcgtcgcg 4141 acgatcccgg tcaagcgcct gggcctgccg gaagagatcg cctcgatctg cgcctggttg 4201 tcgtcggagg agtccggttt ctcgaccggc gccgacttct cgctcaacgg cggcctgcat 4261 atgggctgac ctgc //