LOCUS BBa_K2018035 4273 bp DNA linear 22-Nov-2024 DEFINITION phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB. ACCESSION BBa_K2018035 VERSION BBa_K2018035.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23104 misc_feature 50..53 /label=conserved RBS 44..57 /label=BBa_B0031 misc_feature 1..57 /label=BBa_K608004 CDS 418..2187 /label=phaC1 CDS 2272..3453 /label=phaA CDS 3528..4268 /label=phaB1 promoter 66..115 /label=Pwt RBS 401..407 /label=RBS RBS 2261..2264 /label=RBS RBS 3518..3521 /label=RBS misc_feature 66..4273 /label=BBa_K934001 ORIGIN 1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagtcacaca ggaaacctac 61 tagaggattc ccgcattgac agcgcgtgcg ttgcaaggca acaatggact caaatgtctc 121 ggaatcgctg acgattccca ggtttctccg gcaagcatag cgcatggcgt ctccatgcga 181 gaatgtcgcg cttgccggat aaaaggggag ccgctatcgg aatggacgca agccacggcc 241 gcagcaggtg cggtcgaggg cttccagcca gttccagggc agatgtgccg gcagaccctc 301 ccgctttggg ggaggcgcaa gccgggtcca ttcggatagc atctccccat gcaaagtgcc 361 ggccagggca atgcccggag ccggttcgaa tagtgacggc agagagacaa tcaaatcatg 421 gctactggga aaggagccgc cgccagcacc caggaaggca aaagccaacc atttaaagtg 481 accccaggcc cgttcgatcc tgccacctgg ctggaatggt cacgtcaatg gcaaggtacg 541 gagggcaacg gccatgcggc agcgtccggc atccctgggc ttgatgcact ggctggtgtt 601 aaaattgcgc ctgcccaatt gggagacatc caacagcgtt atatgaaaga tttttcggcg 661 ctgtggcagg cgatggcgga aggtaaagca gaggcgacgg gaccgctgca tgaccgtcgc 721 tttgctggcg acgcctggcg taccaatttg ccctatcgtt ttgcggcagc cttctacctg 781 ctgaacgctc gtgccctgac tgagctggca gatgctgtcg aagcggatgc caaaacccgc 841 caacgcatcc gttttgccat ctcacagtgg gtcgacgcaa tgtcaccggc caacttcctg 901 gccaccaatc cggaagcaca gcgcctcctg attgaaagcg gcggggaatc gctgcgcgcc 961 ggcgttcgta acatgatgga agatctgacc cgtggtaaga tttcgcagac ggatgagtcg 1021 gccttcgagg tgggtcgcaa tgttgcagtg acagaaggcg cggtggtgtt tgagaacgaa 1081 tactttcaac ttctccagta taaaccactg acggataaag tgcatgcgcg cccattgtta 1141 atggttcccc cgtgcattaa taaatactat atcctggatc ttcagccgga aagctcctta 1201 gtgcgccacg tggtggaaca ggggcatact gtgtttttag ttagctggcg caatccagat 1261 gcctcaatgg ccggcagtac ctgggatgac tacattgaac acgcggctat tcgcgcgatc 1321 gaagtggcac gcgacatctc aggtcaggac aagattaacg tactcggctt ttgcgtcggt 1381 ggcactatcg tgagcaccgc tctggcggta ctggctgcgc gtggtgaaca ccccgcagcg 1441 tcggttacgc ttctgaccac actgttggat ttcgccgaca ctggcatctt ggatgtattt 1501 gtcgatgagg gccacgttca gctgcgcgag gccactctgg gtggcggcgc cggcgcaccg 1561 tgcgctctgc tgcgcggtct ggagctcgcg aatacttttt ctttcttacg cccgaacgat 1621 cttgtctgga attacgtagt tgacaactat ctcaaaggta acacgcccgt gccgtttgat 1681 ttattattct ggaatgggga tgcgaccaac ctgccgggtc cttggtattg ttggtatctc 1741 cgtcatacat acttacagaa tgaattaaag gtcccaggta aacttactgt gtgtggtgtc 1801 ccggtagatc tggcgagtat cgacgtgccg acctacattt atgggagtcg tgaagatcat 1861 attgtgccgt ggaccgccgc gtacgcttct accgcgcttc tggcaaacaa attacgtttc 1921 gttctgggtg ccagcggcca cattgcgggt gttattaatc ctccggccaa aaataaacgc 1981 tcgcattgga caaacgacgc cttgccggag agccctcagc agtggttggc gggtgccatt 2041 gagcaccatg gaagttggtg gccggactgg acagcctggc tggcgggcca agcaggcgca 2101 aaacgcgctg cgccagccaa ctatggcaac gcgcgctacc gcgcgattga acccgcgcct 2161 ggccgctatg taaaagcaaa agcgtgacgc ttgcatgagt gccggcgtgc gtcatgcacg 2221 gcgccggcag gcctgcacgt tccctcccgt ttccattgaa aggactacac aatgaccgat 2281 gttgttatcg tcagtgccgc gcgcaccgcc gtgggtaaat ttggcggcag tctggcgaaa 2341 atcccggcgc cagaattggg cgcggtggta atcaaagcgg ccctggaacg cgccggtgtg 2401 aaacctgagc aggtgagcga agtaattatg ggtcaggttc tgaccgcggg cagcgggcaa 2461 aatccggcgc gtcaggctgc gattaaggca ggccttccgg cgatggtgcc cgcgatgacc 2521 attaacaaag tatgcggatc gggactgaaa gcagtgatgc tggccgctaa cgccattatg 2581 gcaggcgacg cggaaattgt ggtcgctggc ggacaggaga atatgtcagc cgcgcctcat 2641 gtgctgccgg gtagccgcga cggctttcgc atgggtgatg caaaactggt cgatactatg 2701 attgttgacg gcttatggga tgtgtacaac cagtaccaca tgggcatcac cgccgagaac 2761 gtggccaagg aatacggcat cacacgcgag gcgcaggatg agttcgccgt cggctcgcag 2821 aacaaggccg aagccgcgca gaaggccggc atgtttgacg aagagatcgt cccggtgctg 2881 atcccgcagc gcaagggcga cccggtggcc ttcaagaccg acgagttcgt gcgccagggc 2941 gccacgctgg acagcatgtc cggcctcaag cccgccttcg acaaggccgg cacggtgacc 3001 gcggccaacg cctcgggcct gaacgacggc gccgccgcgg tggtggtgat gtcggcggcc 3061 aaggccaagg aactgggcct gaccccgctg gccacgatca agagctatgc caacgccggt 3121 gtcgatccca aggtgatggg catgggcccg gtgccggcct ccaagcgcgc cctgtcgcgc 3181 gccgagtgga ccccgcaaga cctggacctg atggagatca acgaggcctt tgccgcgcag 3241 gcgctggcgg tgcaccagca gatgggctgg gacacctcca aggtcaatgt gaacggcggc 3301 gccatcgcca tcggccaccc gatcggcgcg tcgggctgcc gtatcctggt gacgctgctg 3361 cacgagatga agcgccgtga cgcgaagaag ggcctggcct cgctgtgcat cggcggcggc 3421 atgggcgtgg cgctggcagt cgagcgcaaa taaggaaggg gttttccggg gccgcgcgcg 3481 gttggcgcgg acccggcgac gataacgaag ccaatcaagg agtggacatg actcagcgca 3541 ttgcgtatgt gaccggcggc atgggtggta tcggaaccgc catttgccag cggctggcca 3601 aggatggctt tcgtgtggtg gccggttgcg gccccaactc gccgcgccgc gaaaagtggc 3661 tggagcagca gaaggccctg ggcttcgatt tcattgcctc ggaaggcaat gtggctgact 3721 gggactcgac caagaccgca ttcgacaagg tcaagtccga ggtcggcgag gttgatgtgc 3781 tgatcaacaa cgccggtatc acccgcgacg tggtgttccg caagatgacc cgcgccgact 3841 gggatgcggt gatcgacacc aacctgacct cgctgttcaa cgtcaccaag caggtgatcg 3901 acggcatggc cgaccgtggc tggggccgca tcgtcaacat ctcgtcggtg aacgggcaga 3961 agggccagtt cggccagacc aactactcca ccgccaaggc cggcctgcat ggcttcacca 4021 tggcactggc gcaggaagtg gcgaccaagg gcgtgaccgt caacacggtc tctccgggct 4081 atatcgccac cgacatggtc aaggcgatcc gccaggacgt gctcgacaag atcgtcgcga 4141 cgatcccggt caagcgcctg ggcctgccgg aagagatcgc ctcgatctgc gcctggttgt 4201 cgtcggagga gtccggtttc tcgaccggcg ccgacttctc gctcaacggc ggcctgcata 4261 tgggctgacc tgc //