BioBrick that produces large amounts of plastic and secretes it
The BioBrick contains phaCAB with a hybrid promoter and pantothenate kinase II The phaCAB operon does not have a terminator and leaks into the pantothenate kinase gene.
Device with: promoter, rbs, CDS & terminator
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.
Double terminator consisting of BBa_B0010 and BBa_B0012
The sequence comes from Ralstonia eutropha H16 adapted to become a standard BioBrick.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Conversion of the iGEM parts registry to SBOL2.1