BBa_K2018049
type: ComponentDefinition
persistent identity: BBa K2018049
display id: BBa_K2018049
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick that produces bioplastic and secretes it
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T10:20:11Z
mutable provenance: E. coli and R. eutropha.
owned by: james
owned by: myers
mutable description: The biobrick contains phaCAB with a hemolysin based type II secretion system.
discontinued: false
dominant: false
group u list: _2485_
m user id: 30983
owner id: 30983
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Joel Vej-Nielsen
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K2018035
component: BBa_K2018030
sequence annotation: BBa_K2018035
sequence annotation: BBa_K2018030
sequence: https://synbiohub.org/public/igem/BBa_K2018049_sequence/1

BBa_K2018035
type: Component
persistent identity: component2498968
display id: component2498968
version: 1
top level: BBa_K2018049
definition: BBa_K2018035
access: public

BBa_K2018030
type: Component
persistent identity: component2498992
display id: component2498992
version: 1
top level: BBa_K2018049
definition: BBa_K2018030
access: public

BBa_K2018035
type: SequenceAnnotation
persistent identity: annotation2498968
display id: annotation2498968
version: 1
top level: BBa_K2018049
location: https://synbiohub.org/public/igem/BBa_K2018049/annotation2498968/range2498968/1
component: BBa_K2018035

https://synbiohub.org/public/igem/BBa_K2018049/annotation2498968/range2498968/1
type: Range
persistent identity: range2498968
display id: range2498968
version: 1
top level: BBa_K2018049
direction: direction/reverse
start: 1
end: 4273
orientation: inline

BBa_K2018030
type: SequenceAnnotation
persistent identity: annotation2498992
display id: annotation2498992
version: 1
top level: BBa_K2018049
location: https://synbiohub.org/public/igem/BBa_K2018049/annotation2498992/range2498992/1
component: BBa_K2018030

https://synbiohub.org/public/igem/BBa_K2018049/annotation2498992/range2498992/1
type: Range
persistent identity: range2498992
display id: range2498992
version: 1
top level: BBa_K2018049
direction: direction/reverse
start: 4282
end: 9090
orientation: inline

BBa_K2018030
type: ComponentDefinition
persistent identity: BBa K2018030
display id: BBa_K2018030
version: 1
was generated by: iGEM to SBOL conversion
description: Secretion system
created: 2016-06-15T11:00:00Z
modified: 2016-07-06T11:37:09Z
mutable provenance: Escherichia coli & Ralstonia eutropha
owned by: james
owned by: myers
mutable description: Secretes stuff
discontinued: false
dominant: false
group u list: _2485_
m user id: 30983
owner id: 30983
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Joel Vej-Nielsen
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J04500
component: BBa_B0015
component: BBa_K2018029
component: BBa_K2018028
component: BBa_K2018027
component: BBa_K2018026
component: BBa_K2018024
sequence annotation: BBa_J04500
sequence annotation: BBa_K2018024
sequence annotation: BBa_K2018026
sequence annotation: BBa_K2018027
sequence annotation: BBa_K2018028
sequence annotation: BBa_K2018029
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K2018030_sequence/1

BBa_J04500
type: Component
persistent identity: component2480861
display id: component2480861
version: 1
top level: BBa_K2018030
definition: BBa_J04500
access: public

BBa_B0015
type: Component
persistent identity: component2480875
display id: component2480875
version: 1
top level: BBa_K2018030
definition: BBa_B0015
access: public

BBa_K2018029
type: Component
persistent identity: component2480868
display id: component2480868
version: 1
top level: BBa_K2018030
definition: BBa_K2018029
access: public

BBa_K2018028
type: Component
persistent identity: component2480867
display id: component2480867
version: 1
top level: BBa_K2018030
definition: BBa_K2018028
access: public

BBa_K2018027
type: Component
persistent identity: component2480866
display id: component2480866
version: 1
top level: BBa_K2018030
definition: BBa_K2018027
access: public

BBa_K2018026
type: Component
persistent identity: component2480865
display id: component2480865
version: 1
top level: BBa_K2018030
definition: BBa_K2018026
access: public

BBa_K2018024
type: Component
persistent identity: component2480864
display id: component2480864
version: 1
top level: BBa_K2018030
definition: BBa_K2018024
access: public

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2480861
display id: annotation2480861
version: 1
top level: BBa_K2018030
location: https://synbiohub.org/public/igem/BBa_K2018030/annotation2480861/range2480861/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K2018030/annotation2480861/range2480861/1
type: Range
persistent identity: range2480861
display id: range2480861
version: 1
top level: BBa_K2018030
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K2018024
type: SequenceAnnotation
persistent identity: annotation2480864
display id: annotation2480864
version: 1
top level: BBa_K2018030
location: https://synbiohub.org/public/igem/BBa_K2018030/annotation2480864/range2480864/1
component: BBa_K2018024

https://synbiohub.org/public/igem/BBa_K2018030/annotation2480864/range2480864/1
type: Range
persistent identity: range2480864
display id: range2480864
version: 1
top level: BBa_K2018030
direction: direction/reverse
start: 227
end: 988
orientation: inline

BBa_K2018026
type: SequenceAnnotation
persistent identity: annotation2480865
display id: annotation2480865
version: 1
top level: BBa_K2018030
location: https://synbiohub.org/public/igem/BBa_K2018030/annotation2480865/range2480865/1
component: BBa_K2018026

https://synbiohub.org/public/igem/BBa_K2018030/annotation2480865/range2480865/1
type: Range
persistent identity: range2480865
display id: range2480865
version: 1
top level: BBa_K2018030
direction: direction/reverse
start: 997
end: 1067
orientation: inline

BBa_K2018027
type: SequenceAnnotation
persistent identity: annotation2480866
display id: annotation2480866
version: 1
top level: BBa_K2018030
location: https://synbiohub.org/public/igem/BBa_K2018030/annotation2480866/range2480866/1
component: BBa_K2018027

https://synbiohub.org/public/igem/BBa_K2018030/annotation2480866/range2480866/1
type: Range
persistent identity: range2480866
display id: range2480866
version: 1
top level: BBa_K2018030
direction: direction/reverse
start: 1074
end: 3200
orientation: inline

BBa_K2018028
type: SequenceAnnotation
persistent identity: annotation2480867
display id: annotation2480867
version: 1
top level: BBa_K2018030
location: https://synbiohub.org/public/igem/BBa_K2018030/annotation2480867/range2480867/1
component: BBa_K2018028

https://synbiohub.org/public/igem/BBa_K2018030/annotation2480867/range2480867/1
type: Range
persistent identity: range2480867
display id: range2480867
version: 1
top level: BBa_K2018030
direction: direction/reverse
start: 3209
end: 3226
orientation: inline

BBa_K2018029
type: SequenceAnnotation
persistent identity: annotation2480868
display id: annotation2480868
version: 1
top level: BBa_K2018030
location: https://synbiohub.org/public/igem/BBa_K2018030/annotation2480868/range2480868/1
component: BBa_K2018029

https://synbiohub.org/public/igem/BBa_K2018030/annotation2480868/range2480868/1
type: Range
persistent identity: range2480868
display id: range2480868
version: 1
top level: BBa_K2018030
direction: direction/reverse
start: 3233
end: 4672
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2480875
display id: annotation2480875
version: 1
top level: BBa_K2018030
location: https://synbiohub.org/public/igem/BBa_K2018030/annotation2480875/range2480875/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2018030/annotation2480875/range2480875/1
type: Range
persistent identity: range2480875
display id: range2480875
version: 1
top level: BBa_K2018030
direction: direction/reverse
start: 4681
end: 4809
orientation: inline

BBa_K2018035
type: ComponentDefinition
persistent identity: BBa K2018035
display id: BBa_K2018035
version: 1
was generated by: iGEM to SBOL conversion
description: phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.
created: 2016-06-23T11:00:00Z
modified: 2016-07-25T02:47:27Z
mutable provenance: E. coli & R. eutropha Tokyo Tech
owned by: james
owned by: myers
mutable description: bla bla bla
discontinued: false
dominant: false
group u list: _2485_
m user id: 30983
owner id: 30983
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Joel Vej-Nielsen
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K934001
component: BBa_K608004
sequence annotation: BBa_K608004
sequence annotation: BBa_K934001
sequence: https://synbiohub.org/public/igem/BBa_K2018035_sequence/1

BBa_K934001
type: Component
persistent identity: component2481332
display id: component2481332
version: 1
top level: BBa_K2018035
definition: phaC1-A-B1
access: public

BBa_K608004
type: Component
persistent identity: component2481324
display id: component2481324
version: 1
top level: BBa_K2018035
definition: BBa_K608004
access: public

BBa_K608004
type: SequenceAnnotation
persistent identity: annotation2481324
display id: annotation2481324
version: 1
top level: BBa_K2018035
location: https://synbiohub.org/public/igem/BBa_K2018035/annotation2481324/range2481324/1
component: BBa_K608004

https://synbiohub.org/public/igem/BBa_K2018035/annotation2481324/range2481324/1
type: Range
persistent identity: range2481324
display id: range2481324
version: 1
top level: BBa_K2018035
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_K934001
type: SequenceAnnotation
persistent identity: annotation2481332
display id: annotation2481332
version: 1
top level: BBa_K2018035
location: https://synbiohub.org/public/igem/BBa_K2018035/annotation2481332/range2481332/1
component: BBa_K934001

https://synbiohub.org/public/igem/BBa_K2018035/annotation2481332/range2481332/1
type: Range
persistent identity: range2481332
display id: range2481332
version: 1
top level: BBa_K2018035
direction: direction/reverse
start: 66
end: 4273
orientation: inline

BBa_K2018024
type: ComponentDefinition
persistent identity: BBa K2018024
display id: BBa_K2018024
version: 1
was generated by: iGEM to SBOL conversion
description: biofused phaP and hlyA
created: 2016-06-15T11:00:00Z
modified: 2016-06-23T07:41:41Z
mutable provenance: Escherichia coli & Ralstonia eutropha
owned by: james
owned by: myers
mutable description: Encodes phasin protein that is targeted for secretion by the hemolysin pathway.
discontinued: false
dominant: false
group u list: _2485_
m user id: 30983
owner id: 30983
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Joel Vej-Nielsen
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2018023
component: BBa_K2018022
sequence annotation: BBa_K2018022
sequence annotation: BBa_K2018023
sequence: https://synbiohub.org/public/igem/BBa_K2018024_sequence/1

BBa_K2018023
type: Component
persistent identity: component2480465
display id: component2480465
version: 1
top level: BBa_K2018024
definition: BBa_K2018023
access: public

BBa_K2018022
type: Component
persistent identity: component2480464
display id: component2480464
version: 1
top level: BBa_K2018024
definition: BBa_K2018022
access: public

BBa_K2018022
type: SequenceAnnotation
persistent identity: annotation2480464
display id: annotation2480464
version: 1
top level: BBa_K2018024
location: https://synbiohub.org/public/igem/BBa_K2018024/annotation2480464/range2480464/1
component: BBa_K2018022

https://synbiohub.org/public/igem/BBa_K2018024/annotation2480464/range2480464/1
type: Range
persistent identity: range2480464
display id: range2480464
version: 1
top level: BBa_K2018024
direction: direction/reverse
start: 1
end: 576
orientation: inline

BBa_K2018023
type: SequenceAnnotation
persistent identity: annotation2480465
display id: annotation2480465
version: 1
top level: BBa_K2018024
location: https://synbiohub.org/public/igem/BBa_K2018024/annotation2480465/range2480465/1
component: BBa_K2018023

https://synbiohub.org/public/igem/BBa_K2018024/annotation2480465/range2480465/1
type: Range
persistent identity: range2480465
display id: range2480465
version: 1
top level: BBa_K2018024
direction: direction/reverse
start: 577
end: 762
orientation: inline

BBa_K2018026
type: ComponentDefinition
persistent identity: BBa K2018026
display id: BBa_K2018026
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosomal binding site for hlyB
created: 2016-06-15T11:00:00Z
modified: 2016-06-16T07:26:58Z
mutable provenance: Escherichia coli
owned by: james
owned by: myers
mutable description: The native DNA sequence prior to hlyB, including the RBS
discontinued: false
dominant: false
group u list: _2485_
m user id: 30983
owner id: 30983
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Joel Vej-Nielsen
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K2018026_sequence/1

BBa_K2018027
type: ComponentDefinition
persistent identity: BBa K2018027
display id: BBa_K2018027
version: 1
was generated by: iGEM to SBOL conversion
description: hlyB
created: 2016-06-15T11:00:00Z
modified: 2016-06-23T07:37:32Z
mutable provenance: Escherichia coli
owned by: james
owned by: myers
mutable description: Encodes an ATP bindind cassette transporter involved in the alpha hemolysin secretion pathway.
discontinued: false
dominant: false
group u list: _2485_
m user id: 30983
owner id: 30983
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Joel Vej-Nielsen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2018027_sequence/1

BBa_K2018028
type: ComponentDefinition
persistent identity: BBa K2018028
display id: BBa_K2018028
version: 1
was generated by: iGEM to SBOL conversion
description: RBS for hlyD
created: 2016-06-15T11:00:00Z
modified: 2016-06-16T07:32:06Z
mutable provenance: Escherichia coli
owned by: james
owned by: myers
mutable description: Native DNA sequence between hlyB and hlyD containing the ribosomal binding site for hlyD
discontinued: false
dominant: false
group u list: _2485_
m user id: 30983
owner id: 30983
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Joel Vej-Nielsen
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K2018028_sequence/1

BBa_K2018029
type: ComponentDefinition
persistent identity: BBa K2018029
display id: BBa_K2018029
version: 1
was generated by: iGEM to SBOL conversion
description: hlyD
created: 2016-06-15T11:00:00Z
modified: 2016-06-23T07:14:01Z
mutable provenance: Escherichia coli
owned by: james
owned by: myers
mutable description: Encodes a Holin that functions in the hemolysin secretion pathway.
discontinued: false
dominant: false
group u list: _2485_
m user id: 30983
owner id: 30983
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Joel Vej-Nielsen
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2018029_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K608004
type: ComponentDefinition
persistent identity: BBa K608004
display id: BBa_K608004
version: 1
was generated by: iGEM to SBOL conversion
description: strong Promoter , weak RBS
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
mutable provenance: composite
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: strong Promotor , weak RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: composite
star: false
creator: Julia M??ller
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0031
component: BBa_J23104
sequence annotation: BBa_J23104
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_K608004_sequence/1

BBa_B0031
type: Component
persistent identity: component2128655
display id: component2128655
version: 1
top level: BBa_K608004
definition: BBa_B0031
access: public

BBa_J23104
type: Component
persistent identity: component2128653
display id: component2128653
version: 1
top level: BBa_K608004
definition: BBa_J23104
access: public

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2128653
display id: annotation2128653
version: 1
top level: BBa_K608004
location: https://synbiohub.org/public/igem/BBa_K608004/annotation2128653/range2128653/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K608004/annotation2128653/range2128653/1
type: Range
persistent identity: range2128653
display id: range2128653
version: 1
top level: BBa_K608004
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2128655
display id: annotation2128655
version: 1
top level: BBa_K608004
location: https://synbiohub.org/public/igem/BBa_K608004/annotation2128655/range2128655/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K608004/annotation2128655/range2128655/1
type: Range
persistent identity: range2128655
display id: range2128655
version: 1
top level: BBa_K608004
direction: direction/reverse
start: 44
end: 57
orientation: inline

phaC1-A-B1
type: ComponentDefinition
persistent identity: BBa K934001
display id: BBa_K934001
version: 1
was generated by: iGEM to SBOL conversion
description: phaC1-A-B1 [P(3HB) synthesis]
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:47Z
mutable provenance: test
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The sequence comes from Ralstonia eutropha H16 adapted to become a standard BioBrick.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1199_
m user id: 0
owner id: 14016
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: test
star: false
creator: Taku Nakayama
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Pwt
sequence annotation: RBS
sequence annotation: phaC1
sequence annotation: phaA
sequence annotation: phaB1
sequence: https://synbiohub.org/public/igem/BBa_K934001_sequence/1

Pwt
type: SequenceAnnotation
persistent identity: annotation2192328
display id: annotation2192328
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192328/range2192328/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K934001/annotation2192328/range2192328/1
type: Range
persistent identity: range2192328
display id: range2192328
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 1
end: 50
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192329
display id: annotation2192329
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192329/range2192329/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K934001/annotation2192329/range2192329/1
type: Range
persistent identity: range2192329
display id: range2192329
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 336
end: 342
orientation: inline

phaC1
type: SequenceAnnotation
persistent identity: annotation2192325
display id: annotation2192325
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192325/range2192325/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K934001/annotation2192325/range2192325/1
type: Range
persistent identity: range2192325
display id: range2192325
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 353
end: 2122
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192330
display id: annotation2192330
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192330/range2192330/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K934001/annotation2192330/range2192330/1
type: Range
persistent identity: range2192330
display id: range2192330
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 2196
end: 2199
orientation: inline

phaA
type: SequenceAnnotation
persistent identity: annotation2192326
display id: annotation2192326
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192326/range2192326/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K934001/annotation2192326/range2192326/1
type: Range
persistent identity: range2192326
display id: range2192326
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 2207
end: 3388
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192331
display id: annotation2192331
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192331/range2192331/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K934001/annotation2192331/range2192331/1
type: Range
persistent identity: range2192331
display id: range2192331
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 3453
end: 3456
orientation: inline

phaB1
type: SequenceAnnotation
persistent identity: annotation2192327
display id: annotation2192327
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192327/range2192327/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K934001/annotation2192327/range2192327/1
type: Range
persistent identity: range2192327
display id: range2192327
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 3463
end: 4203
orientation: inline

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_K2018022
type: ComponentDefinition
persistent identity: BBa K2018022
display id: BBa_K2018022
version: 1
was generated by: iGEM to SBOL conversion
description: The gene encoding phasin
created: 2016-06-15T11:00:00Z
modified: 2016-06-16T06:58:36Z
mutable provenance: Ralstonia eutropha
owned by: james
owned by: myers
mutable description: Phasin is a protein that binds to the surface of poly hydroxy alkanoate granules.
discontinued: false
dominant: false
group u list: _2485_
m user id: 30983
owner id: 30983
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Joel Vej-Nielsen
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2018022_sequence/1

BBa_K2018023
type: ComponentDefinition
persistent identity: BBa K2018023
display id: BBa_K2018023
version: 1
was generated by: iGEM to SBOL conversion
description: hlyA secretion tag
created: 2016-06-15T11:00:00Z
modified: 2016-06-23T07:14:33Z
mutable provenance: Escherichia coli
owned by: james
owned by: myers
mutable description: The last part of the gene from hemolysin toxin, which marks the protein for secretion by the type II hemolysin pathway
discontinued: false
dominant: false
experience: experience/None
group u list: _2485_
m user id: 30983
owner id: 30983
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Doesn't contain a start codon as it is meant for biofusion
star: false
creator: Joel Vej-Nielsen
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K2018023_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
mutable description: replace later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

https://synbiohub.org/public/igem/BBa_K2018049_sequence/1
type: Sequence
persistent identity: BBa K2018049 sequence
display id: BBa_K2018049_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaacctactagaggattcccgcattgacagcgcgtgcgttgcaaggcaacaatggactcaaatgtctcggaatcgctgacgattcccaggtttctccggcaagcatagcgcatggcgtctccatgcgagaatgtcgcgcttgccggataaaaggggagccgctatcggaatggacgcaagccacggccgcagcaggtgcggtcgagggcttccagccagttccagggcagatgtgccggcagaccctcccgctttgggggaggcgcaagccgggtccattcggatagcatctccccatgcaaagtgccggccagggcaatgcccggagccggttcgaatagtgacggcagagagacaatcaaatcatggctactgggaaaggagccgccgccagcacccaggaaggcaaaagccaaccatttaaagtgaccccaggcccgttcgatcctgccacctggctggaatggtcacgtcaatggcaaggtacggagggcaacggccatgcggcagcgtccggcatccctgggcttgatgcactggctggtgttaaaattgcgcctgcccaattgggagacatccaacagcgttatatgaaagatttttcggcgctgtggcaggcgatggcggaaggtaaagcagaggcgacgggaccgctgcatgaccgtcgctttgctggcgacgcctggcgtaccaatttgccctatcgttttgcggcagccttctacctgctgaacgctcgtgccctgactgagctggcagatgctgtcgaagcggatgccaaaacccgccaacgcatccgttttgccatctcacagtgggtcgacgcaatgtcaccggccaacttcctggccaccaatccggaagcacagcgcctcctgattgaaagcggcggggaatcgctgcgcgccggcgttcgtaacatgatggaagatctgacccgtggtaagatttcgcagacggatgagtcggccttcgaggtgggtcgcaatgttgcagtgacagaaggcgcggtggtgtttgagaacgaatactttcaacttctccagtataaaccactgacggataaagtgcatgcgcgcccattgttaatggttcccccgtgcattaataaatactatatcctggatcttcagccggaaagctccttagtgcgccacgtggtggaacaggggcatactgtgtttttagttagctggcgcaatccagatgcctcaatggccggcagtacctgggatgactacattgaacacgcggctattcgcgcgatcgaagtggcacgcgacatctcaggtcaggacaagattaacgtactcggcttttgcgtcggtggcactatcgtgagcaccgctctggcggtactggctgcgcgtggtgaacaccccgcagcgtcggttacgcttctgaccacactgttggatttcgccgacactggcatcttggatgtatttgtcgatgagggccacgttcagctgcgcgaggccactctgggtggcggcgccggcgcaccgtgcgctctgctgcgcggtctggagctcgcgaatactttttctttcttacgcccgaacgatcttgtctggaattacgtagttgacaactatctcaaaggtaacacgcccgtgccgtttgatttattattctggaatggggatgcgaccaacctgccgggtccttggtattgttggtatctccgtcatacatacttacagaatgaattaaaggtcccaggtaaacttactgtgtgtggtgtcccggtagatctggcgagtatcgacgtgccgacctacatttatgggagtcgtgaagatcatattgtgccgtggaccgccgcgtacgcttctaccgcgcttctggcaaacaaattacgtttcgttctgggtgccagcggccacattgcgggtgttattaatcctccggccaaaaataaacgctcgcattggacaaacgacgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2018030_sequence/1
type: Sequence
persistent identity: BBa K2018030 sequence
display id: BBa_K2018030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagccgcaggtagcttcgatgttaaagaggaaagaactgccgcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcatagtaatactagagtatattaatttaaatgatagcaatcttactgggctgtgccacataagattgctattttttttggagtcatatactagatggattcttgtcataaaattgattatgggttatacgccctggagattttagcccaataccataacgtctctgttaacccggaagaaattaaacatagatttgatacagacgggacaggtctgggattaacgtcatggttgcttgctgcgaaatctttagaactaaaggtaaaacaggtaaaaaaaacaattgatcgattaaactttatttttctgcccgcattagtctggagagaggatggacgtcattttattctgactaaaatcagcaaagaagtaaacagatatcttatttttgatttggagcagcgaaatccccgtgttctcgaacagtctgagtttgaggcgttatatcaggggcatattattcttattacttcccgttcttctgttaccgggaaactggcaaaatttgactttacctggtttattcctgccattataaaatacaggagaatatttattgaaacccttgttgtatctgtttttttacaattatttgcattaataaccccccttttttttcaggtggttatggacaaagtattagtgcacagggggttttcaacccttaatgttattactgttgcattatctgttgtagtggtgtttgagattatactcagcggtttaagaacttacatttttgcacatagtacaagtcggattgatgttgagttgggtgccaaactcttccggcatttactggcgctaccgatctcttattttgagagtcgtcgtgttggtgatactgttgcgagggtaagagaattagaccagatccgtaattttctgacaggacaggcattaacatctgttttggacttattattttcactcatattttttgcggtaatgtggtattacagcccaaagcttactctggtgatcttattttcgctgccttgttatgctgcatggtctgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2018035_sequence/1
type: Sequence
persistent identity: BBa K2018035 sequence
display id: BBa_K2018035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaacctactagaggattcccgcattgacagcgcgtgcgttgcaaggcaacaatggactcaaatgtctcggaatcgctgacgattcccaggtttctccggcaagcatagcgcatggcgtctccatgcgagaatgtcgcgcttgccggataaaaggggagccgctatcggaatggacgcaagccacggccgcagcaggtgcggtcgagggcttccagccagttccagggcagatgtgccggcagaccctcccgctttgggggaggcgcaagccgggtccattcggatagcatctccccatgcaaagtgccggccagggcaatgcccggagccggttcgaatagtgacggcagagagacaatcaaatcatggctactgggaaaggagccgccgccagcacccaggaaggcaaaagccaaccatttaaagtgaccccaggcccgttcgatcctgccacctggctggaatggtcacgtcaatggcaaggtacggagggcaacggccatgcggcagcgtccggcatccctgggcttgatgcactggctggtgttaaaattgcgcctgcccaattgggagacatccaacagcgttatatgaaagatttttcggcgctgtggcaggcgatggcggaaggtaaagcagaggcgacgggaccgctgcatgaccgtcgctttgctggcgacgcctggcgtaccaatttgccctatcgttttgcggcagccttctacctgctgaacgctcgtgccctgactgagctggcagatgctgtcgaagcggatgccaaaacccgccaacgcatccgttttgccatctcacagtgggtcgacgcaatgtcaccggccaacttcctggccaccaatccggaagcacagcgcctcctgattgaaagcggcggggaatcgctgcgcgccggcgttcgtaacatgatggaagatctgacccgtggtaagatttcgcagacggatgagtcggccttcgaggtgggtcgcaatgttgcagtgacagaaggcgcggtggtgtttgagaacgaatactttcaacttctccagtataaaccactgacggataaagtgcatgcgcgcccattgttaatggttcccccgtgcattaataaatactatatcctggatcttcagccggaaagctccttagtgcgccacgtggtggaacaggggcatactgtgtttttagttagctggcgcaatccagatgcctcaatggccggcagtacctgggatgactacattgaacacgcggctattcgcgcgatcgaagtggcacgcgacatctcaggtcaggacaagattaacgtactcggcttttgcgtcggtggcactatcgtgagcaccgctctggcggtactggctgcgcgtggtgaacaccccgcagcgtcggttacgcttctgaccacactgttggatttcgccgacactggcatcttggatgtatttgtcgatgagggccacgttcagctgcgcgaggccactctgggtggcggcgccggcgcaccgtgcgctctgctgcgcggtctggagctcgcgaatactttttctttcttacgcccgaacgatcttgtctggaattacgtagttgacaactatctcaaaggtaacacgcccgtgccgtttgatttattattctggaatggggatgcgaccaacctgccgggtccttggtattgttggtatctccgtcatacatacttacagaatgaattaaaggtcccaggtaaacttactgtgtgtggtgtcccggtagatctggcgagtatcgacgtgccgacctacatttatgggagtcgtgaagatcatattgtgccgtggaccgccgcgtacgcttctaccgcgcttctggcaaacaaattacgtttcgttctgggtgccagcggccacattgcgggtgttattaatcctccggccaaaaataaacgctcgcattggacaaacgacgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2018024_sequence/1
type: Sequence
persistent identity: BBa K2018024 sequence
display id: BBa_K2018024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagccgcaggtagcttcgatgttaaagaggaaagaactgccgcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcatagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2018026_sequence/1
type: Sequence
persistent identity: BBa K2018026 sequence
display id: BBa_K2018026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatattaatttaaatgatagcaatcttactgggctgtgccacataagattgctattttttttggagtcata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2018027_sequence/1
type: Sequence
persistent identity: BBa K2018027 sequence
display id: BBa_K2018027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggattcttgtcataaaattgattatgggttatacgccctggagattttagcccaataccataacgtctctgttaacccggaagaaattaaacatagatttgatacagacgggacaggtctgggattaacgtcatggttgcttgctgcgaaatctttagaactaaaggtaaaacaggtaaaaaaaacaattgatcgattaaactttatttttctgcccgcattagtctggagagaggatggacgtcattttattctgactaaaatcagcaaagaagtaaacagatatcttatttttgatttggagcagcgaaatccccgtgttctcgaacagtctgagtttgaggcgttatatcaggggcatattattcttattacttcccgttcttctgttaccgggaaactggcaaaatttgactttacctggtttattcctgccattataaaatacaggagaatatttattgaaacccttgttgtatctgtttttttacaattatttgcattaataaccccccttttttttcaggtggttatggacaaagtattagtgcacagggggttttcaacccttaatgttattactgttgcattatctgttgtagtggtgtttgagattatactcagcggtttaagaacttacatttttgcacatagtacaagtcggattgatgttgagttgggtgccaaactcttccggcatttactggcgctaccgatctcttattttgagagtcgtcgtgttggtgatactgttgcgagggtaagagaattagaccagatccgtaattttctgacaggacaggcattaacatctgttttggacttattattttcactcatattttttgcggtaatgtggtattacagcccaaagcttactctggtgatcttattttcgctgccttgttatgctgcatggtctgtttttattagccccattttgcgacgtcgccttgatgataagttttcacggaatgcggataatcaatctttcctggtggaatcagtaacggcgattaacactataaaagctatggcagtctcacctcagatgacgaacatatgggacaaacaattggcaggatatgttgccgcaggctttaaagtgacagtattagcaaccattggtcaacaaggaatacagttaatacaaaagactgttatgatcatcaacctatggttgggagcacacctggttatttccggggatttaagtattggtcagttaattgcttttaatatgcttgctggtcagattgttgcaccggttattcgccttgcacaaatctggcaggatttccagcaggttggtatatcagttacccgccttggtgatgtgcttaactctccaactgaaagttatcatgggaaactgacattgccggaaattaatggtgatatcacttttcgtaatatccggtttcgctataaacctgattctccggttattttggacaatatcaatcttagtattaagcagggggaggttattggtattgtcggacgttctggttcaggaaaaagcacattaactaaattaattcaacgtttttatattcctgaaaatggccaggtattaattgatggacatgatcttgcgttggctgatcctaactggttacgtcgtcaggtgggggttgtgttgcaggacaatgtgctgcttaatcgcagtattattgataatatttcactggctaatcctggcatgtccgtcgaaaaagttatttatgcagcgaaattagcaggcgctcatgattttatttctgatttgcgtgaggggtataacaccattgtcggggaacagggggcaggattatccggaggtcaacgtcaacgcatcgcaattgcaagggcgctggtgaacaaccctaaaatactcatttttgatgaagcaaccagtgctctggattatgagtcggagcatgtcatcatgcgcaatatgcacaaaatatgtaagggcagaacggttataatcattgctcatcgtctgtctacagt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2018028_sequence/1
type: Sequence
persistent identity: BBa K2018028 sequence
display id: BBa_K2018028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagaaagaacagaagaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2018029_sequence/1
type: Sequence
persistent identity: BBa K2018029 sequence
display id: BBa_K2018029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaacatggttaatggggttcagcgagttcctgttgcgctataaacttgtctggagtgaaacatggaaaatccggaagcaattagatactccggtacgtgaaaaggacgaaaatgaatttttacccgctcatctggaattaattgaaacgccagtatccagacggccgcgtctggttgcttattttattatggggtttctggttattgcttttattttatctgttttaggccaagtggaaattgttgccactgcaaatgggaaattaacacacagtgggcgtagtaaagaaattaaacctattgaaaactcaatagttaaagaaattatcgtaaaagaaggagagtcagtccggaaaggggatgtgttattaaagcttacagcactgggagctgaagctgatacgttaaaaacacagtcatcactgttacaggccaggctggaacaaactcggtatcaaattctgagcaggtcaattgaattaaataaactacctgaactaaagcttcctgatgagccttattttcagaatgtatctgaagaggaagtactgcgtttaacttctttgataaaagaacagttttccacatggcaaaatcagaagtatcaaaaagaactgaatttggataagaaaagagcagagcgattaacagtacttgcccgtataaaccgttatgaaaatttatcaagggttgaaaaaagccgtctggatgatttcagtagtttattgcataaacaggcaattgcaaaacatgctgtacttgagcaggagaataaatatgtcgaagcagtaaatgaattacgagtttataaatcacaactggagcaaattgagagtgagatattgtctgcaaaagaagaatatcagcttgttacgcagctttttaaaaatgaaattttagataagctaagacaaacaacagacaacattgggttattaactctggaattagcgaaaaatgaagagcgtcaacaggcttcagtaatcagggccccagtttcgggaaaagttcagcaactgaaggttcatactgaaggtggggttgttacaacagcggaaacactgatggtcatcgttccggaagatgacacgctggaggttactgctctggtacaaaataaagatattggttttattaacgtcgggcagaatgccatcattaaagtggaggcatttccttatacacgatatggttatctggtgggtaaggtgaaaaatataaatttagatgcaatagaagaccagagactgggacttgtttttaatgttattatttctattgaagagaattgtttgtcaaccgggaataaaaacattccattaagctcgggtatggcagtcactgccgaaataaagacaggtatgcgaagtgtaatcagttatcttcttagtcctttagaagagtcagtaacagaaagtttacgtgagcgttagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K608004_sequence/1
type: Sequence
persistent identity: BBa K608004 sequence
display id: BBa_K608004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K934001_sequence/1
type: Sequence
persistent identity: BBa K934001 sequence
display id: BBa_K934001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcccgcattgacagcgcgtgcgttgcaaggcaacaatggactcaaatgtctcggaatcgctgacgattcccaggtttctccggcaagcatagcgcatggcgtctccatgcgagaatgtcgcgcttgccggataaaaggggagccgctatcggaatggacgcaagccacggccgcagcaggtgcggtcgagggcttccagccagttccagggcagatgtgccggcagaccctcccgctttgggggaggcgcaagccgggtccattcggatagcatctccccatgcaaagtgccggccagggcaatgcccggagccggttcgaatagtgacggcagagagacaatcaaatcatggctactgggaaaggagccgccgccagcacccaggaaggcaaaagccaaccatttaaagtgaccccaggcccgttcgatcctgccacctggctggaatggtcacgtcaatggcaaggtacggagggcaacggccatgcggcagcgtccggcatccctgggcttgatgcactggctggtgttaaaattgcgcctgcccaattgggagacatccaacagcgttatatgaaagatttttcggcgctgtggcaggcgatggcggaaggtaaagcagaggcgacgggaccgctgcatgaccgtcgctttgctggcgacgcctggcgtaccaatttgccctatcgttttgcggcagccttctacctgctgaacgctcgtgccctgactgagctggcagatgctgtcgaagcggatgccaaaacccgccaacgcatccgttttgccatctcacagtgggtcgacgcaatgtcaccggccaacttcctggccaccaatccggaagcacagcgcctcctgattgaaagcggcggggaatcgctgcgcgccggcgttcgtaacatgatggaagatctgacccgtggtaagatttcgcagacggatgagtcggccttcgaggtgggtcgcaatgttgcagtgacagaaggcgcggtggtgtttgagaacgaatactttcaacttctccagtataaaccactgacggataaagtgcatgcgcgcccattgttaatggttcccccgtgcattaataaatactatatcctggatcttcagccggaaagctccttagtgcgccacgtggtggaacaggggcatactgtgtttttagttagctggcgcaatccagatgcctcaatggccggcagtacctgggatgactacattgaacacgcggctattcgcgcgatcgaagtggcacgcgacatctcaggtcaggacaagattaacgtactcggcttttgcgtcggtggcactatcgtgagcaccgctctggcggtactggctgcgcgtggtgaacaccccgcagcgtcggttacgcttctgaccacactgttggatttcgccgacactggcatcttggatgtatttgtcgatgagggccacgttcagctgcgcgaggccactctgggtggcggcgccggcgcaccgtgcgctctgctgcgcggtctggagctcgcgaatactttttctttcttacgcccgaacgatcttgtctggaattacgtagttgacaactatctcaaaggtaacacgcccgtgccgtttgatttattattctggaatggggatgcgaccaacctgccgggtccttggtattgttggtatctccgtcatacatacttacagaatgaattaaaggtcccaggtaaacttactgtgtgtggtgtcccggtagatctggcgagtatcgacgtgccgacctacatttatgggagtcgtgaagatcatattgtgccgtggaccgccgcgtacgcttctaccgcgcttctggcaaacaaattacgtttcgttctgggtgccagcggccacattgcgggtgttattaatcctccggccaaaaataaacgctcgcattggacaaacgacgccttgccggagagccctcagcagtggttggcgggtgccattgagcaccatggaagttggtggccgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2018022_sequence/1
type: Sequence
persistent identity: BBa K2018022 sequence
display id: BBa_K2018022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2018023_sequence/1
type: Sequence
persistent identity: BBa K2018023 sequence
display id: BBa_K2018023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagccgcaggtagcttcgatgttaaagaggaaagaactgccgcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcatagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23104_sequence/1
type: Sequence
persistent identity: BBa J23104 sequence
display id: BBa_J23104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z