LOCUS BBa_K2018050 10134 bp DNA linear 15-Jun-2024 DEFINITION BioBrick that produces large amounts of plastic and secretes it ACCESSION BBa_K2018050 VERSION BBa_K2018050.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23104 misc_feature 50..53 /label=conserved RBS 44..57 /label=BBa_B0031 misc_feature 1..57 /label=BBa_K608004 CDS 418..2187 /label=phaC1 CDS 2272..3453 /label=phaA CDS 3528..4268 /label=phaB1 promoter 66..115 /label=Pwt RBS 401..407 /label=RBS RBS 2261..2264 /label=RBS RBS 3518..3521 /label=RBS misc_feature 66..4273 /label=BBa_K934001 misc_feature 1..4273 /label=BBa_K2018035 CDS 4282..4369 /label=end of LacI coding region (inactive) misc_feature 4282..4481 /label=BBa_R0010 misc_feature 4370..4407 /label=CAP binding site promoter 4418..4423 /label=-35 promoter 4442..4447 /label=-10 misc_feature 4447..4481 /label=LacI binding site misc_feature 4454..4454 /label=start promoter 4282..4481 /label=BBa_R0010 misc_feature 4494..4497 /label=conserved RBS 4490..4501 /label=BBa_B0034 misc_feature 4282..4501 /label=BBa_J04500 CDS 4508..5083 /label=BBa_K2018022 misc_feature 5084..5269 /label=BBa_K2018023 misc_feature 4508..5269 /label=BBa_K2018024 RBS 5278..5348 /label=BBa_K2018026 CDS 5355..7481 /label=BBa_K2018027 RBS 7490..7507 /label=BBa_K2018028 CDS 7514..8953 /label=BBa_K2018029 stem_loop 8973..9016 /label=stem_loop misc_feature 8962..9041 /label=BBa_B0010 terminator 8962..9041 /label=BBa_B0010 stem_loop 9057..9076 /label=T7 TE misc_feature 9083..9083 /label=stop polyA_site 9077..9090 /label=polya misc_feature 9050..9090 /label=BBa_B0012 terminator 9050..9090 /label=BBa_B0012 terminator 8962..9090 /label=BBa_B0015 misc_feature 4282..9090 /label=BBa_K2018030 promoter 9099..9142 /label=BBa_K2018031 RBS 9143..9163 /label=BBa_K2018032 CDS 9164..10005 /label=BBa_K2018020 stem_loop 10017..10060 /label=stem_loop misc_feature 10006..10085 /label=BBa_B0010 terminator 10006..10085 /label=BBa_B0010 stem_loop 10101..10120 /label=T7 TE misc_feature 10127..10127 /label=stop polyA_site 10121..10134 /label=polya misc_feature 10094..10134 /label=BBa_B0012 terminator 10094..10134 /label=BBa_B0012 terminator 10006..10134 /label=BBa_B0015 misc_feature 9099..10134 /label=BBa_K2018021 ORIGIN 1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagtcacaca ggaaacctac 61 tagaggattc ccgcattgac agcgcgtgcg ttgcaaggca acaatggact caaatgtctc 121 ggaatcgctg acgattccca ggtttctccg gcaagcatag cgcatggcgt ctccatgcga 181 gaatgtcgcg cttgccggat aaaaggggag ccgctatcgg aatggacgca agccacggcc 241 gcagcaggtg cggtcgaggg cttccagcca gttccagggc agatgtgccg gcagaccctc 301 ccgctttggg ggaggcgcaa gccgggtcca ttcggatagc atctccccat gcaaagtgcc 361 ggccagggca atgcccggag ccggttcgaa tagtgacggc agagagacaa tcaaatcatg 421 gctactggga aaggagccgc cgccagcacc caggaaggca aaagccaacc atttaaagtg 481 accccaggcc cgttcgatcc tgccacctgg ctggaatggt cacgtcaatg gcaaggtacg 541 gagggcaacg gccatgcggc agcgtccggc atccctgggc ttgatgcact ggctggtgtt 601 aaaattgcgc ctgcccaatt gggagacatc caacagcgtt atatgaaaga tttttcggcg 661 ctgtggcagg cgatggcgga aggtaaagca gaggcgacgg gaccgctgca tgaccgtcgc 721 tttgctggcg acgcctggcg taccaatttg ccctatcgtt ttgcggcagc cttctacctg 781 ctgaacgctc gtgccctgac tgagctggca gatgctgtcg aagcggatgc caaaacccgc 841 caacgcatcc gttttgccat ctcacagtgg gtcgacgcaa tgtcaccggc caacttcctg 901 gccaccaatc cggaagcaca gcgcctcctg attgaaagcg gcggggaatc gctgcgcgcc 961 ggcgttcgta acatgatgga agatctgacc cgtggtaaga tttcgcagac ggatgagtcg 1021 gccttcgagg tgggtcgcaa tgttgcagtg acagaaggcg cggtggtgtt tgagaacgaa 1081 tactttcaac ttctccagta taaaccactg acggataaag tgcatgcgcg cccattgtta 1141 atggttcccc cgtgcattaa taaatactat atcctggatc ttcagccgga aagctcctta 1201 gtgcgccacg tggtggaaca ggggcatact gtgtttttag ttagctggcg caatccagat 1261 gcctcaatgg ccggcagtac ctgggatgac tacattgaac acgcggctat tcgcgcgatc 1321 gaagtggcac gcgacatctc aggtcaggac aagattaacg tactcggctt ttgcgtcggt 1381 ggcactatcg tgagcaccgc tctggcggta ctggctgcgc gtggtgaaca ccccgcagcg 1441 tcggttacgc ttctgaccac actgttggat ttcgccgaca ctggcatctt ggatgtattt 1501 gtcgatgagg gccacgttca gctgcgcgag gccactctgg gtggcggcgc cggcgcaccg 1561 tgcgctctgc tgcgcggtct ggagctcgcg aatacttttt ctttcttacg cccgaacgat 1621 cttgtctgga attacgtagt tgacaactat ctcaaaggta acacgcccgt gccgtttgat 1681 ttattattct ggaatgggga tgcgaccaac ctgccgggtc cttggtattg ttggtatctc 1741 cgtcatacat acttacagaa tgaattaaag gtcccaggta aacttactgt gtgtggtgtc 1801 ccggtagatc tggcgagtat cgacgtgccg acctacattt atgggagtcg tgaagatcat 1861 attgtgccgt ggaccgccgc gtacgcttct accgcgcttc tggcaaacaa attacgtttc 1921 gttctgggtg ccagcggcca cattgcgggt gttattaatc ctccggccaa aaataaacgc 1981 tcgcattgga caaacgacgc cttgccggag agccctcagc agtggttggc gggtgccatt 2041 gagcaccatg gaagttggtg gccggactgg acagcctggc tggcgggcca agcaggcgca 2101 aaacgcgctg cgccagccaa ctatggcaac gcgcgctacc gcgcgattga acccgcgcct 2161 ggccgctatg taaaagcaaa agcgtgacgc ttgcatgagt gccggcgtgc gtcatgcacg 2221 gcgccggcag gcctgcacgt tccctcccgt ttccattgaa aggactacac aatgaccgat 2281 gttgttatcg tcagtgccgc gcgcaccgcc gtgggtaaat ttggcggcag tctggcgaaa 2341 atcccggcgc cagaattggg cgcggtggta atcaaagcgg ccctggaacg cgccggtgtg 2401 aaacctgagc aggtgagcga agtaattatg ggtcaggttc tgaccgcggg cagcgggcaa 2461 aatccggcgc gtcaggctgc gattaaggca ggccttccgg cgatggtgcc cgcgatgacc 2521 attaacaaag tatgcggatc gggactgaaa gcagtgatgc tggccgctaa cgccattatg 2581 gcaggcgacg cggaaattgt ggtcgctggc ggacaggaga atatgtcagc cgcgcctcat 2641 gtgctgccgg gtagccgcga cggctttcgc atgggtgatg caaaactggt cgatactatg 2701 attgttgacg gcttatggga tgtgtacaac cagtaccaca tgggcatcac cgccgagaac 2761 gtggccaagg aatacggcat cacacgcgag gcgcaggatg agttcgccgt cggctcgcag 2821 aacaaggccg aagccgcgca gaaggccggc atgtttgacg aagagatcgt cccggtgctg 2881 atcccgcagc gcaagggcga cccggtggcc ttcaagaccg acgagttcgt gcgccagggc 2941 gccacgctgg acagcatgtc cggcctcaag cccgccttcg acaaggccgg cacggtgacc 3001 gcggccaacg cctcgggcct gaacgacggc gccgccgcgg tggtggtgat gtcggcggcc 3061 aaggccaagg aactgggcct gaccccgctg gccacgatca agagctatgc caacgccggt 3121 gtcgatccca aggtgatggg catgggcccg gtgccggcct ccaagcgcgc cctgtcgcgc 3181 gccgagtgga ccccgcaaga cctggacctg atggagatca acgaggcctt tgccgcgcag 3241 gcgctggcgg tgcaccagca gatgggctgg gacacctcca aggtcaatgt gaacggcggc 3301 gccatcgcca tcggccaccc gatcggcgcg tcgggctgcc gtatcctggt gacgctgctg 3361 cacgagatga agcgccgtga cgcgaagaag ggcctggcct cgctgtgcat cggcggcggc 3421 atgggcgtgg cgctggcagt cgagcgcaaa taaggaaggg gttttccggg gccgcgcgcg 3481 gttggcgcgg acccggcgac gataacgaag ccaatcaagg agtggacatg actcagcgca 3541 ttgcgtatgt gaccggcggc atgggtggta tcggaaccgc catttgccag cggctggcca 3601 aggatggctt tcgtgtggtg gccggttgcg gccccaactc gccgcgccgc gaaaagtggc 3661 tggagcagca gaaggccctg ggcttcgatt tcattgcctc ggaaggcaat gtggctgact 3721 gggactcgac caagaccgca ttcgacaagg tcaagtccga ggtcggcgag gttgatgtgc 3781 tgatcaacaa cgccggtatc acccgcgacg tggtgttccg caagatgacc cgcgccgact 3841 gggatgcggt gatcgacacc aacctgacct cgctgttcaa cgtcaccaag caggtgatcg 3901 acggcatggc cgaccgtggc tggggccgca tcgtcaacat ctcgtcggtg aacgggcaga 3961 agggccagtt cggccagacc aactactcca ccgccaaggc cggcctgcat ggcttcacca 4021 tggcactggc gcaggaagtg gcgaccaagg gcgtgaccgt caacacggtc tctccgggct 4081 atatcgccac cgacatggtc aaggcgatcc gccaggacgt gctcgacaag atcgtcgcga 4141 cgatcccggt caagcgcctg ggcctgccgg aagagatcgc ctcgatctgc gcctggttgt 4201 cgtcggagga gtccggtttc tcgaccggcg ccgacttctc gctcaacggc ggcctgcata 4261 tgggctgacc tgctactaga gcaatacgca aaccgcctct ccccgcgcgt tggccgattc 4321 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 4381 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 4441 gtatgttgtg tggaattgtg agcggataac aatttcacac atactagaga aagaggagaa 4501 atactagatg atcctcaccc cggaacaagt tgcagcagcg caaaaggcca acctcgaaac 4561 gctgttcggc ctgaccacca aggcgtttga aggcgtcgaa aagctcgtcg agctgaacct 4621 tcaggtcgtc aagacttcgt tcgcagaagg cgttgacaac gccaagaagg cgctgtcggc 4681 caaggacgca caggaactgc tggccatcca ggccgcagcc gtgcagccgg ttgccgaaaa 4741 gaccctggcc tacacccgcc acctgtatga aatcgcttcg gaaacccaga gcgagttcac 4801 caaggtagcc gaggctcaac tggccgaagg ctcgaagaac gtgcaagcgc tggtcgagaa 4861 cctcgccaag aacgccccgg ccggttcgga atcgaccgtg gccatcgtga agtcggcgat 4921 ctccgctgcc aacaacgcct acgagtcggt gcagaaggcg accaagcaag cggtcgaaat 4981 cgctgaaacc aacttccagg ctgcggctac ggctgccacc aaggctgccc agcaagccag 5041 cgccacggcc cgtacggcca cggcaaagaa gacgacggct gccttagcct atggaagtca 5101 gggtgatctt aatccattaa ttaatgaaat cagcaaaatc atttcagccg caggtagctt 5161 cgatgttaaa gaggaaagaa ctgccgcttc tttattgcag ttgtccggta atgccagtga 5221 tttttcatat ggacggaact caataaccct gaccacatca gcatagtaat actagagtat 5281 attaatttaa atgatagcaa tcttactggg ctgtgccaca taagattgct attttttttg 5341 gagtcatata ctagatggat tcttgtcata aaattgatta tgggttatac gccctggaga 5401 ttttagccca ataccataac gtctctgtta acccggaaga aattaaacat agatttgata 5461 cagacgggac aggtctggga ttaacgtcat ggttgcttgc tgcgaaatct ttagaactaa 5521 aggtaaaaca ggtaaaaaaa acaattgatc gattaaactt tatttttctg cccgcattag 5581 tctggagaga ggatggacgt cattttattc tgactaaaat cagcaaagaa gtaaacagat 5641 atcttatttt tgatttggag cagcgaaatc cccgtgttct cgaacagtct gagtttgagg 5701 cgttatatca ggggcatatt attcttatta cttcccgttc ttctgttacc gggaaactgg 5761 caaaatttga ctttacctgg tttattcctg ccattataaa atacaggaga atatttattg 5821 aaacccttgt tgtatctgtt tttttacaat tatttgcatt aataaccccc cttttttttc 5881 aggtggttat ggacaaagta ttagtgcaca gggggttttc aacccttaat gttattactg 5941 ttgcattatc tgttgtagtg gtgtttgaga ttatactcag cggtttaaga acttacattt 6001 ttgcacatag tacaagtcgg attgatgttg agttgggtgc caaactcttc cggcatttac 6061 tggcgctacc gatctcttat tttgagagtc gtcgtgttgg tgatactgtt gcgagggtaa 6121 gagaattaga ccagatccgt aattttctga caggacaggc attaacatct gttttggact 6181 tattattttc actcatattt tttgcggtaa tgtggtatta cagcccaaag cttactctgg 6241 tgatcttatt ttcgctgcct tgttatgctg catggtctgt ttttattagc cccattttgc 6301 gacgtcgcct tgatgataag ttttcacgga atgcggataa tcaatctttc ctggtggaat 6361 cagtaacggc gattaacact ataaaagcta tggcagtctc acctcagatg acgaacatat 6421 gggacaaaca attggcagga tatgttgccg caggctttaa agtgacagta ttagcaacca 6481 ttggtcaaca aggaatacag ttaatacaaa agactgttat gatcatcaac ctatggttgg 6541 gagcacacct ggttatttcc ggggatttaa gtattggtca gttaattgct tttaatatgc 6601 ttgctggtca gattgttgca ccggttattc gccttgcaca aatctggcag gatttccagc 6661 aggttggtat atcagttacc cgccttggtg atgtgcttaa ctctccaact gaaagttatc 6721 atgggaaact gacattgccg gaaattaatg gtgatatcac ttttcgtaat atccggtttc 6781 gctataaacc tgattctccg gttattttgg acaatatcaa tcttagtatt aagcaggggg 6841 aggttattgg tattgtcgga cgttctggtt caggaaaaag cacattaact aaattaattc 6901 aacgttttta tattcctgaa aatggccagg tattaattga tggacatgat cttgcgttgg 6961 ctgatcctaa ctggttacgt cgtcaggtgg gggttgtgtt gcaggacaat gtgctgctta 7021 atcgcagtat tattgataat atttcactgg ctaatcctgg catgtccgtc gaaaaagtta 7081 tttatgcagc gaaattagca ggcgctcatg attttatttc tgatttgcgt gaggggtata 7141 acaccattgt cggggaacag ggggcaggat tatccggagg tcaacgtcaa cgcatcgcaa 7201 ttgcaagggc gctggtgaac aaccctaaaa tactcatttt tgatgaagca accagtgctc 7261 tggattatga gtcggagcat gtcatcatgc gcaatatgca caaaatatgt aagggcagaa 7321 cggttataat cattgctcat cgtctgtcta cagtaaaaaa tgcagaccgc attattgtca 7381 tggaaaaagg gaaaattgtt gaacagggta aacataagga gctgctttct gaaccggaaa 7441 gtttatacag ttacttatat cagttacagt cagactaata gtactagagc agaaagaaca 7501 gaagaattac tagatgaaaa catggttaat ggggttcagc gagttcctgt tgcgctataa 7561 acttgtctgg agtgaaacat ggaaaatccg gaagcaatta gatactccgg tacgtgaaaa 7621 ggacgaaaat gaatttttac ccgctcatct ggaattaatt gaaacgccag tatccagacg 7681 gccgcgtctg gttgcttatt ttattatggg gtttctggtt attgctttta ttttatctgt 7741 tttaggccaa gtggaaattg ttgccactgc aaatgggaaa ttaacacaca gtgggcgtag 7801 taaagaaatt aaacctattg aaaactcaat agttaaagaa attatcgtaa aagaaggaga 7861 gtcagtccgg aaaggggatg tgttattaaa gcttacagca ctgggagctg aagctgatac 7921 gttaaaaaca cagtcatcac tgttacaggc caggctggaa caaactcggt atcaaattct 7981 gagcaggtca attgaattaa ataaactacc tgaactaaag cttcctgatg agccttattt 8041 tcagaatgta tctgaagagg aagtactgcg tttaacttct ttgataaaag aacagttttc 8101 cacatggcaa aatcagaagt atcaaaaaga actgaatttg gataagaaaa gagcagagcg 8161 attaacagta cttgcccgta taaaccgtta tgaaaattta tcaagggttg aaaaaagccg 8221 tctggatgat ttcagtagtt tattgcataa acaggcaatt gcaaaacatg ctgtacttga 8281 gcaggagaat aaatatgtcg aagcagtaaa tgaattacga gtttataaat cacaactgga 8341 gcaaattgag agtgagatat tgtctgcaaa agaagaatat cagcttgtta cgcagctttt 8401 taaaaatgaa attttagata agctaagaca aacaacagac aacattgggt tattaactct 8461 ggaattagcg aaaaatgaag agcgtcaaca ggcttcagta atcagggccc cagtttcggg 8521 aaaagttcag caactgaagg ttcatactga aggtggggtt gttacaacag cggaaacact 8581 gatggtcatc gttccggaag atgacacgct ggaggttact gctctggtac aaaataaaga 8641 tattggtttt attaacgtcg ggcagaatgc catcattaaa gtggaggcat ttccttatac 8701 acgatatggt tatctggtgg gtaaggtgaa aaatataaat ttagatgcaa tagaagacca 8761 gagactggga cttgttttta atgttattat ttctattgaa gagaattgtt tgtcaaccgg 8821 gaataaaaac attccattaa gctcgggtat ggcagtcact gccgaaataa agacaggtat 8881 gcgaagtgta atcagttatc ttcttagtcc tttagaagag tcagtaacag aaagtttacg 8941 tgagcgttag taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 9001 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 9061 caccttcggg tgggcctttc tgcgtttata tactagagag attgacagct agctcagtcc 9121 taggtattgt gctagctact agagtcacac aggaaagtac tagatgaaag tcggtatcga 9181 tgctggtggc accctgatta agatcgtgca ggaacaggat aaccagcgta cctttaaaac 9241 tgaactgacc aagaacatcg accaggtcgt ggaatggctg aaccagcagc agattgaaaa 9301 gctgtgcctg accggcggca acgctggtgt tatcgccgaa aacatcaaca ttccggcgca 9361 aatctttgtg gagttcgatg cggcttccca gggtctgggc attctgctga aagaacaagg 9421 tcatgacctg gccgactaca tcttcgcaaa cgtcggcact ggcaccagcc tgcactactt 9481 tgacggccag agccagcgcc gcgttggtgg tatcggcacc ggtggcggta tgatccaggg 9541 cctgggttac ctgctgtctc agattaccga ttacaaacag ctgactgaca tggcgcagca 9601 cggtgaccgt aacacgattg atctgaaagt gcgtcacatc tataaagata ccgaaccgcc 9661 gatcccaggc gacctgactg ctgcgaactt tggtcatgtg ctgcatcacc tggatgccga 9721 cttcacccct tccaacaaac tggcagcggt tattggcgtt gtcggtgaag tggtgaccac 9781 catggctatc actgttgcac gtgagttcaa aacggaaaat attgtgtaca ttggtagcag 9841 cttccacaac aatgcgctgc tgcgcaaagt tgtcgaagac tacaccgtgc tgcgtggctg 9901 caaaccttat tatgtagaaa acggtgcgtt ctctggcgct atcggcgctc tgtatctgga 9961 aaaggggtct gactacaaag acgatgacga caagtgatac tagagccagg catcaaataa 10021 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 10081 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //