LOCUS BBa_K2019000 3183 bp DNA linear 26-Nov-2024 DEFINITION Histidine Tagged WT Staphylococcus Aureus Cas9 Protein ACCESSION BBa_K2019000 VERSION BBa_K2019000.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=his-6 CDS 22..3183 /label=saCas9 ORIGIN 1 atgcaccacc atcatcatca tatgaagaga aattacattc tgggattaga tataggcatt 61 actagcgttg ggtacggaat aatcgactac gagacacgcg acgttataga cgctggcgtg 121 cgcttgttta aagaagctaa tgtggaaaac aatgaaggga gacgtagtaa acgcggtgcc 181 cgccgtttga aaagaagacg ccggcacaga atacagcgcg ttaagaagtt gcttttcgac 241 tataacttat tgacagacca ttcagagctt tctggtataa acccctatga agcacgcgtt 301 aagggacttt ctcagaagct ttcggaagaa gagttcagtg ccgcattgtt gcaccttgca 361 aagagacgtg gtgtccacaa cgtgaatgag gtcgaggaag acacagggaa tgaattgtca 421 acaaaagaac agatatcacg gaactcgaaa gccctggaag aaaaatacgt cgccgagtta 481 cagctggagc ggttgaagaa ggacggtgaa gtacggggtt ctataaatcg gttcaaaacc 541 agcgattacg taaaagaagc gaagcagctt ctgaaggtcc agaaagcata ccaccagctg 601 gaccaatctt tcattgacac ctatatagat ctgcttgaaa cacgcagaac ttactatgag 661 ggtcccggag agggtagccc gttcggttgg aaggatatca aggaatggta tgaaatgttg 721 atgggacact gtacatactt cccggaagaa cttcggtcag taaaatatgc atacaacgca 781 gatctttaca atgccctgaa tgatctgaat aacttagtga tcacacggga cgagaatgaa 841 aagttggagt actacgaaaa gttccaaatt attgaaaatg tgtttaagca aaagaagaaa 901 cctacactga agcaaattgc caaagagatc ttagtcaacg aagaggacat aaaaggttat 961 cgcgtcacct cgaccggcaa acccgagttc accaatttga aggtttatca cgacataaag 1021 gacatcaccg cgagaaaaga gatcatagag aacgcagagt tactggacca gattgctaaa 1081 attttaacca tatatcagag tagcgaggac atacaggaag aattaacgaa tcttaatagt 1141 gagcttaccc aggaggagat cgagcaaatt tcgaacctga agggatatac aggcacacac 1201 aacctttcac tgaaggcgat aaatctgatt ttagacgaac tgtggcacac aaacgacaat 1261 caaattgcca tattcaatcg tcttaagttg gtgccgaaaa aagtcgatct gtcgcagcag 1321 aaggaaatcc ctacgacctt agtagatgat tttatcttaa gccccgtagt taagcgcagt 1381 ttcattcaaa gcataaaagt cataaatgcg atcatcaaga agtatgggct tccgaacgac 1441 ataattatcg aattggctag agaaaagaac tcgaaagatg cgcaaaagat gatcaacgag 1501 atgcaaaaac gcaaccggca gacgaacgag cgtattgaag agatcattag aaccacaggg 1561 aaagaaaatg ccaagtatct gatagagaag attaaattac acgacatgca agaaggaaaa 1621 tgcttgtata gtttggaggc catcccgctt gaggatctgt tgaataatcc tttcaactac 1681 gaggtggatc acataatacc ccgtagcgtt tcattcgata attcttttaa taataaggta 1741 cttgtcaagc aggaggagaa tagtaaaaag ggtaaccgca cgccatttca atatttgtcc 1801 tcgtctgatt cgaaaatttc gtacgaaacc tttaagaaac acattttgaa cttggcaaag 1861 gggaaaggac ggatttcaaa aacaaagaag gaataccttt tggaagaacg cgacataaac 1921 agatttagcg tgcagaaaga ctttataaac cggaacttag ttgacactcg gtacgctacg 1981 cggggcttaa tgaacctttt acggtcctac ttccgggtta ataatcttga tgtgaaagtt 2041 aagagcataa atggtggatt tacatcgttt ttgcggcgta aatggaaatt caagaaggag 2101 cggaataagg gttataaaca ccacgcagaa gatgccctta taatagcgaa cgcagatttt 2161 atcttcaagg aatggaagaa gctggataag gcgaagaaag tcatggagaa tcagatgttt 2221 gaggaaaaac aagcagagag catgccggaa atcgagacag agcaggagta taaagagatt 2281 tttataactc ctcaccaaat aaagcatatt aaagatttta aagattataa atattcacat 2341 agagtggata aaaaacccaa tcgggaatta atcaatgaca cgttgtatag taccagaaaa 2401 gacgataagg ggaatacctt aattgtcaac aatttaaacg gcctgtacga caaggataac 2461 gataaactta aaaagctgat caataaaagc ccagaaaaat tgctgatgta ccatcacgac 2521 cctcagacct accaaaaatt aaaattgatt atggaacaat acggtgacga gaagaacccg 2581 ttgtataaat attatgaaga gacgggcaat tatttaacta aatactcgaa gaaggataac 2641 gggcccgtca ttaagaagat caaatactat ggcaacaaat tgaatgcaca tcttgatata 2701 accgatgatt accccaattc acgcaataaa gtagtcaaac tgtctttgaa gccgtaccgg 2761 tttgatgttt acttggataa tggggtgtat aagtttgtca cagtcaaaaa cctggatgtc 2821 attaaaaaag aaaactacta tgaagtgaat agtaaatgct acgaagaggc aaaaaagctt 2881 aaaaagataa gcaatcaagc agaatttata gcaagtttct ataataatga tcttattaaa 2941 ataaacggcg aactttaccg cgtcataggg gtgaataacg accttcttaa tagaatagag 3001 gtcaatatga tcgatattac ataccgcgaa tatttagaaa atatgaatga taagcgcccc 3061 cctcgcatta tcaagactat agcatccaag acgcagtcca ttaagaaata ctccacggac 3121 atccttggca acctttacga agttaagtct aaaaaacatc cgcagattat taaaaagggc 3181 tga //