BBa_K2019000 1 BBa_K2019000 Histidine Tagged WT Staphylococcus Aureus Cas9 Protein 2016-07-14T11:00:00Z 2016-10-14T08:19:07Z Tet promoter was used from the . SaCas9 was extracted from the genomic sequence of staphylococcus aureus and then codon optimized for E. Coli. Part BBa_K2019000 expresses crispr associated protein 9 extracted from staphylococcus aureus (saCas9) in E. Coli. saCas9 is much smaller than the typical cas9 extracted form the genome of streptococcus thermophilus (spCas9), as it the gene that encodes for saCas9 is ~1000bp shorter than that of spCas9. Furthermore, saCas9 has been shown to cut just as efficiently as spCas9 while also offering a more specific PAM sequence of NNGRRT. The his tag (his6) was attached to saCas9 to allow one to able to run a FPLC test. false false _2486_ 31051 31051 9 In stock true We linearized a backbone with the tet promoter and then used gibson assembly to attach two gblocks with the Cas9 sequence. false John Lazar annotation2494541 1 saCas9 range2494541 1 22 3183 annotation2494532 1 his-6 range2494532 1 1 21 BBa_K2019000_sequence 1 atgcaccaccatcatcatcatatgaagagaaattacattctgggattagatataggcattactagcgttgggtacggaataatcgactacgagacacgcgacgttatagacgctggcgtgcgcttgtttaaagaagctaatgtggaaaacaatgaagggagacgtagtaaacgcggtgcccgccgtttgaaaagaagacgccggcacagaatacagcgcgttaagaagttgcttttcgactataacttattgacagaccattcagagctttctggtataaacccctatgaagcacgcgttaagggactttctcagaagctttcggaagaagagttcagtgccgcattgttgcaccttgcaaagagacgtggtgtccacaacgtgaatgaggtcgaggaagacacagggaatgaattgtcaacaaaagaacagatatcacggaactcgaaagccctggaagaaaaatacgtcgccgagttacagctggagcggttgaagaaggacggtgaagtacggggttctataaatcggttcaaaaccagcgattacgtaaaagaagcgaagcagcttctgaaggtccagaaagcataccaccagctggaccaatctttcattgacacctatatagatctgcttgaaacacgcagaacttactatgagggtcccggagagggtagcccgttcggttggaaggatatcaaggaatggtatgaaatgttgatgggacactgtacatacttcccggaagaacttcggtcagtaaaatatgcatacaacgcagatctttacaatgccctgaatgatctgaataacttagtgatcacacgggacgagaatgaaaagttggagtactacgaaaagttccaaattattgaaaatgtgtttaagcaaaagaagaaacctacactgaagcaaattgccaaagagatcttagtcaacgaagaggacataaaaggttatcgcgtcacctcgaccggcaaacccgagttcaccaatttgaaggtttatcacgacataaaggacatcaccgcgagaaaagagatcatagagaacgcagagttactggaccagattgctaaaattttaaccatatatcagagtagcgaggacatacaggaagaattaacgaatcttaatagtgagcttacccaggaggagatcgagcaaatttcgaacctgaagggatatacaggcacacacaacctttcactgaaggcgataaatctgattttagacgaactgtggcacacaaacgacaatcaaattgccatattcaatcgtcttaagttggtgccgaaaaaagtcgatctgtcgcagcagaaggaaatccctacgaccttagtagatgattttatcttaagccccgtagttaagcgcagtttcattcaaagcataaaagtcataaatgcgatcatcaagaagtatgggcttccgaacgacataattatcgaattggctagagaaaagaactcgaaagatgcgcaaaagatgatcaacgagatgcaaaaacgcaaccggcagacgaacgagcgtattgaagagatcattagaaccacagggaaagaaaatgccaagtatctgatagagaagattaaattacacgacatgcaagaaggaaaatgcttgtatagtttggaggccatcccgcttgaggatctgttgaataatcctttcaactacgaggtggatcacataataccccgtagcgtttcattcgataattcttttaataataaggtacttgtcaagcaggaggagaatagtaaaaagggtaaccgcacgccatttcaatatttgtcctcgtctgattcgaaaatttcgtacgaaacctttaagaaacacattttgaacttggcaaaggggaaaggacggatttcaaaaacaaagaaggaataccttttggaagaacgcgacataaacagatttagcgtgcagaaagactttataaaccggaacttagttgacactcggtacgctacgcggggcttaatgaaccttttacggtcctacttccgggttaataatcttgatgtgaaagttaagagcataaatggtggatttacatcgtttttgcggcgtaaatggaaattcaagaaggagcggaataagggttataaacaccacgcagaagatgcccttataatagcgaacgcagattttatcttcaaggaatggaagaagctggataaggcgaagaaagtcatggagaatcagatgtttgaggaaaaacaagcagagagcatgccggaaatcgagacagagcaggagtataaagagatttttataactcctcaccaaataaagcatattaaagattttaaagattataaatattcacatagagtggataaaaaacccaatcgggaattaatcaatgacacgttgtatagtaccagaaaagacgataaggggaataccttaattgtcaacaatttaaacggcctgtacgacaaggataacgataaacttaaaaagctgatcaataaaagcccagaaaaattgctgatgtaccatcacgaccctcagacctaccaaaaattaaaattgattatggaacaatacggtgacgagaagaacccgttgtataaatattatgaagagacgggcaattatttaactaaatactcgaagaaggataacgggcccgtcattaagaagatcaaatactatggcaacaaattgaatgcacatcttgatataaccgatgattaccccaattcacgcaataaagtagtcaaactgtctttgaagccgtaccggtttgatgtttacttggataatggggtgtataagtttgtcacagtcaaaaacctggatgtcattaaaaaagaaaactactatgaagtgaatagtaaatgctacgaagaggcaaaaaagcttaaaaagataagcaatcaagcagaatttatagcaagtttctataataatgatcttattaaaataaacggcgaactttaccgcgtcataggggtgaataacgaccttcttaatagaatagaggtcaatatgatcgatattacataccgcgaatatttagaaaatatgaatgataagcgcccccctcgcattatcaagactatagcatccaagacgcagtccattaagaaatactccacggacatccttggcaacctttacgaagttaagtctaaaaaacatccgcagattattaaaaagggctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z