BBa_K2019001 1 BBa_K2019001 mRFP+gRNA Template (for saCas9) 2016-07-14T11:00:00Z 2016-10-15T12:29:42Z Tet promoter was used from the. SaCas9 was extracted from the genomic sequence of staphylococcus aureus and then codon optimized for E. Coli. Part BBa_K2019000 expresses crispr associated protein 9 extracted from staphylococcus aureus (saCas9) in E. Coli. saCas9 is much smaller than the typical cas9 extracted form the genome of streptococcus thermophilus (spCas9), as it the gene that encodes for saCas9 is ~1000bp shorter than that of spCas9. Furthermore, saCas9 has been shown to cut just as efficiently as spCas9 while also offering a more specific PAM sequence of NNGRRT. The his tag (his6) was attached to saCas9 to allow one to able to run a FPLC test. false false _2486_ 31051 31051 9 false We linearized a backbone with the tet promoter and then used gibson assembly to attach two gblocks with the Cas9 sequence. false John Lazar annotation2511939 1 BBa_J23119 range2511939 1 1069 1104 annotation2511940 1 gRNA Template range2511940 1 1104 1160 annotation2511941 1 gRNA Scaffolding range2511941 1 1161 1243 annotation2511938 1 BBa_J04450 range2511938 1 1 1068 BBa_K2019001_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatattgacagctagctcagtcctaggtataatactagtggagacccatgccatagcgttgttcggaacagattcaccaacacctagtggtctcggttttagtactctggaaacagaatctactaaaacaaggcaaaatgccgtgtttatctcgtcaacttgttggcgagattttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z