LOCUS BBa_K2019003 3309 bp DNA linear 22-Nov-2024 DEFINITION Periplasm Directed saCas9 via TorA Signaling Sequence (TorA-saCas9) ACCESSION BBa_K2019003 VERSION BBa_K2019003.1 FEATURES Location/Qualifiers ORIGIN 1 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc 61 ttaaccgtgg ccgggatgct ggggccgtca ttgttaacgc cgcgacgtgc gactgcggcg 121 caagcgatgc accaccatca tcatcatatg aagagaaatt acattctggg attagatata 181 ggcattacta gcgttgggta cggaataatc gactacgaga cacgcgacgt tatagacgct 241 ggcgtgcgct tgtttaaaga agctaatgtg gaaaacaatg aagggagacg tagtaaacgc 301 ggtgcccgcc gtttgaaaag aagacgccgg cacagaatac agcgcgttaa gaagttgctt 361 ttcgactata acttattgac agaccattca gagctttctg gtataaaccc ctatgaagca 421 cgcgttaagg gactttctca gaagctttcg gaagaagagt tcagtgccgc attgttgcac 481 cttgcaaaga gacgtggtgt ccacaacgtg aatgaggtcg aggaagacac agggaatgaa 541 ttgtcaacaa aagaacagat atcacggaac tcgaaagccc tggaagaaaa atacgtcgcc 601 gagttacagc tggagcggtt gaagaaggac ggtgaagtac ggggttctat aaatcggttc 661 aaaaccagcg attacgtaaa agaagcgaag cagcttctga aggtccagaa agcataccac 721 cagctggacc aatctttcat tgacacctat atagatctgc ttgaaacacg cagaacttac 781 tatgagggtc ccggagaggg tagcccgttc ggttggaagg atatcaagga atggtatgaa 841 atgttgatgg gacactgtac atacttcccg gaagaacttc ggtcagtaaa atatgcatac 901 aacgcagatc tttacaatgc cctgaatgat ctgaataact tagtgatcac acgggacgag 961 aatgaaaagt tggagtacta cgaaaagttc caaattattg aaaatgtgtt taagcaaaag 1021 aagaaaccta cactgaagca aattgccaaa gagatcttag tcaacgaaga ggacataaaa 1081 ggttatcgcg tcacctcgac cggcaaaccc gagttcacca atttgaaggt ttatcacgac 1141 ataaaggaca tcaccgcgag aaaagagatc atagagaacg cagagttact ggaccagatt 1201 gctaaaattt taaccatata tcagagtagc gaggacatac aggaagaatt aacgaatctt 1261 aatagtgagc ttacccagga ggagatcgag caaatttcga acctgaaggg atatacaggc 1321 acacacaacc tttcactgaa ggcgataaat ctgattttag acgaactgtg gcacacaaac 1381 gacaatcaaa ttgccatatt caatcgtctt aagttggtgc cgaaaaaagt cgatctgtcg 1441 cagcagaagg aaatccctac gaccttagta gatgatttta tcttaagccc cgtagttaag 1501 cgcagtttca ttcaaagcat aaaagtcata aatgcgatca tcaagaagta tgggcttccg 1561 aacgacataa ttatcgaatt ggctagagaa aagaactcga aagatgcgca aaagatgatc 1621 aacgagatgc aaaaacgcaa ccggcagacg aacgagcgta ttgaagagat cattagaacc 1681 acagggaaag aaaatgccaa gtatctgata gagaagatta aattacacga catgcaagaa 1741 ggaaaatgct tgtatagttt ggaggccatc ccgcttgagg atctgttgaa taatcctttc 1801 aactacgagg tggatcacat aataccccgt agcgtttcat tcgataattc ttttaataat 1861 aaggtacttg tcaagcagga ggagaatagt aaaaagggta accgcacgcc atttcaatat 1921 ttgtcctcgt ctgattcgaa aatttcgtac gaaaccttta agaaacacat tttgaacttg 1981 gcaaagggga aaggacggat ttcaaaaaca aagaaggaat accttttgga agaacgcgac 2041 ataaacagat ttagcgtgca gaaagacttt ataaaccgga acttagttga cactcggtac 2101 gctacgcggg gcttaatgaa ccttttacgg tcctacttcc gggttaataa tcttgatgtg 2161 aaagttaaga gcataaatgg tggatttaca tcgtttttgc ggcgtaaatg gaaattcaag 2221 aaggagcgga ataagggtta taaacaccac gcagaagatg cccttataat agcgaacgca 2281 gattttatct tcaaggaatg gaagaagctg gataaggcga agaaagtcat ggagaatcag 2341 atgtttgagg aaaaacaagc agagagcatg ccggaaatcg agacagagca ggagtataaa 2401 gagattttta taactcctca ccaaataaag catattaaag attttaaaga ttataaatat 2461 tcacatagag tggataaaaa acccaatcgg gaattaatca atgacacgtt gtatagtacc 2521 agaaaagacg ataaggggaa taccttaatt gtcaacaatt taaacggcct gtacgacaag 2581 gataacgata aacttaaaaa gctgatcaat aaaagcccag aaaaattgct gatgtaccat 2641 cacgaccctc agacctacca aaaattaaaa ttgattatgg aacaatacgg tgacgagaag 2701 aacccgttgt ataaatatta tgaagagacg ggcaattatt taactaaata ctcgaagaag 2761 gataacgggc ccgtcattaa gaagatcaaa tactatggca acaaattgaa tgcacatctt 2821 gatataaccg atgattaccc caattcacgc aataaagtag tcaaactgtc tttgaagccg 2881 taccggtttg atgtttactt ggataatggg gtgtataagt ttgtcacagt caaaaacctg 2941 gatgtcatta aaaaagaaaa ctactatgaa gtgaatagta aatgctacga agaggcaaaa 3001 aagcttaaaa agataagcaa tcaagcagaa tttatagcaa gtttctataa taatgatctt 3061 attaaaataa acggcgaact ttaccgcgtc ataggggtga ataacgacct tcttaataga 3121 atagaggtca atatgatcga tattacatac cgcgaatatt tagaaaatat gaatgataag 3181 cgcccccctc gcattatcaa gactatagca tccaagacgc agtccattaa gaaatactcc 3241 acggacatcc ttggcaacct ttacgaagtt aagtctaaaa aacatccgca gattattaaa 3301 aagggctga //