BBa_K2019003 1 BBa_K2019003 Periplasm Directed saCas9 via TorA Signaling Sequence (TorA-saCas9) 2016-10-11T11:00:00Z 2016-10-12T08:30:50Z The TorA sequence is from part BBa_K1012002 of the 2013 Dundee Team. The saCas9 sequence is from another Northwestern 2016 iGEM part, BBa_K2019000. TorA-Cas9 is a fusion of the TorA signaling sequence to the N-terminus of staphylococcus aureus CRISPR associated protein 9 (saCas9). TorA utilizes the twin arginine translocation (tat) pathway to direct the fused protein to the periplasm of the cell. Therefore, by fusing Cas9 to the C-terminus of the signaling sequence, it should be directed to the periplasm of the cell. false false _2486_ 31051 31051 9 false Part BBa_K1012002 was ordered as a gblock with homology to the BBa_K2019000-BBa_R0040 expression system. BBa_K2019000-BBa_R0040 was linearized at the 5' end of saCas9. A 2 piece gibson assembly was then performed with the gblock and Cas9 expression backbone. false John Lazar BBa_K2019003_sequence 1 atgaacaataacgatctctttcaggcatcacgtcggcgttttctggcacaactcggcggcttaaccgtggccgggatgctggggccgtcattgttaacgccgcgacgtgcgactgcggcgcaagcgatgcaccaccatcatcatcatatgaagagaaattacattctgggattagatataggcattactagcgttgggtacggaataatcgactacgagacacgcgacgttatagacgctggcgtgcgcttgtttaaagaagctaatgtggaaaacaatgaagggagacgtagtaaacgcggtgcccgccgtttgaaaagaagacgccggcacagaatacagcgcgttaagaagttgcttttcgactataacttattgacagaccattcagagctttctggtataaacccctatgaagcacgcgttaagggactttctcagaagctttcggaagaagagttcagtgccgcattgttgcaccttgcaaagagacgtggtgtccacaacgtgaatgaggtcgaggaagacacagggaatgaattgtcaacaaaagaacagatatcacggaactcgaaagccctggaagaaaaatacgtcgccgagttacagctggagcggttgaagaaggacggtgaagtacggggttctataaatcggttcaaaaccagcgattacgtaaaagaagcgaagcagcttctgaaggtccagaaagcataccaccagctggaccaatctttcattgacacctatatagatctgcttgaaacacgcagaacttactatgagggtcccggagagggtagcccgttcggttggaaggatatcaaggaatggtatgaaatgttgatgggacactgtacatacttcccggaagaacttcggtcagtaaaatatgcatacaacgcagatctttacaatgccctgaatgatctgaataacttagtgatcacacgggacgagaatgaaaagttggagtactacgaaaagttccaaattattgaaaatgtgtttaagcaaaagaagaaacctacactgaagcaaattgccaaagagatcttagtcaacgaagaggacataaaaggttatcgcgtcacctcgaccggcaaacccgagttcaccaatttgaaggtttatcacgacataaaggacatcaccgcgagaaaagagatcatagagaacgcagagttactggaccagattgctaaaattttaaccatatatcagagtagcgaggacatacaggaagaattaacgaatcttaatagtgagcttacccaggaggagatcgagcaaatttcgaacctgaagggatatacaggcacacacaacctttcactgaaggcgataaatctgattttagacgaactgtggcacacaaacgacaatcaaattgccatattcaatcgtcttaagttggtgccgaaaaaagtcgatctgtcgcagcagaaggaaatccctacgaccttagtagatgattttatcttaagccccgtagttaagcgcagtttcattcaaagcataaaagtcataaatgcgatcatcaagaagtatgggcttccgaacgacataattatcgaattggctagagaaaagaactcgaaagatgcgcaaaagatgatcaacgagatgcaaaaacgcaaccggcagacgaacgagcgtattgaagagatcattagaaccacagggaaagaaaatgccaagtatctgatagagaagattaaattacacgacatgcaagaaggaaaatgcttgtatagtttggaggccatcccgcttgaggatctgttgaataatcctttcaactacgaggtggatcacataataccccgtagcgtttcattcgataattcttttaataataaggtacttgtcaagcaggaggagaatagtaaaaagggtaaccgcacgccatttcaatatttgtcctcgtctgattcgaaaatttcgtacgaaacctttaagaaacacattttgaacttggcaaaggggaaaggacggatttcaaaaacaaagaaggaataccttttggaagaacgcgacataaacagatttagcgtgcagaaagactttataaaccggaacttagttgacactcggtacgctacgcggggcttaatgaaccttttacggtcctacttccgggttaataatcttgatgtgaaagttaagagcataaatggtggatttacatcgtttttgcggcgtaaatggaaattcaagaaggagcggaataagggttataaacaccacgcagaagatgcccttataatagcgaacgcagattttatcttcaaggaatggaagaagctggataaggcgaagaaagtcatggagaatcagatgtttgaggaaaaacaagcagagagcatgccggaaatcgagacagagcaggagtataaagagatttttataactcctcaccaaataaagcatattaaagattttaaagattataaatattcacatagagtggataaaaaacccaatcgggaattaatcaatgacacgttgtatagtaccagaaaagacgataaggggaataccttaattgtcaacaatttaaacggcctgtacgacaaggataacgataaacttaaaaagctgatcaataaaagcccagaaaaattgctgatgtaccatcacgaccctcagacctaccaaaaattaaaattgattatggaacaatacggtgacgagaagaacccgttgtataaatattatgaagagacgggcaattatttaactaaatactcgaagaaggataacgggcccgtcattaagaagatcaaatactatggcaacaaattgaatgcacatcttgatataaccgatgattaccccaattcacgcaataaagtagtcaaactgtctttgaagccgtaccggtttgatgtttacttggataatggggtgtataagtttgtcacagtcaaaaacctggatgtcattaaaaaagaaaactactatgaagtgaatagtaaatgctacgaagaggcaaaaaagcttaaaaagataagcaatcaagcagaatttatagcaagtttctataataatgatcttattaaaataaacggcgaactttaccgcgtcataggggtgaataacgaccttcttaatagaatagaggtcaatatgatcgatattacataccgcgaatatttagaaaatatgaatgataagcgcccccctcgcattatcaagactatagcatccaagacgcagtccattaagaaatactccacggacatccttggcaacctttacgaagttaagtctaaaaaacatccgcagattattaaaaagggctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z