LOCUS BBa_K2019004 3339 bp DNA linear 26-Nov-2024 DEFINITION Periplasm Directed saCas9 via DsbA Signaling Sequence (DsbA-saCas9) ACCESSION BBa_K2019004 VERSION BBa_K2019004.1 FEATURES Location/Qualifiers ORIGIN 1 agcgataggt acttacgtgc tcacaactct atccagggat tcgagtcaag cagcatcaca 61 acgtcaagac gcaaccacag cgataggtac ttacgtgcta tgaaaaaaat ttggctcgcc 121 ctggctggtg ctgttttagc gtttagcgca tcggcgatgc accaccatca tcatcatatg 181 aagagaaatt acattctggg attagatata ggcattacta gcgttgggta cggaataatc 241 gactacgaga cacgcgacgt tatagacgct ggcgtgcgct tgtttaaaga agctaatgtg 301 gaaaacaatg aagggagacg tagtaaacgc ggtgcccgcc gtttgaaaag aagacgccgg 361 cacagaatac agcgcgttaa gaagttgctt ttcgactata acttattgac agaccattca 421 gagctttctg gtataaaccc ctatgaagca cgcgttaagg gactttctca gaagctttcg 481 gaagaagagt tcagtgccgc attgttgcac cttgcaaaga gacgtggtgt ccacaacgtg 541 aatgaggtcg aggaagacac agggaatgaa ttgtcaacaa aagaacagat atcacggaac 601 tcgaaagccc tggaagaaaa atacgtcgcc gagttacagc tggagcggtt gaagaaggac 661 ggtgaagtac ggggttctat aaatcggttc aaaaccagcg attacgtaaa agaagcgaag 721 cagcttctga aggtccagaa agcataccac cagctggacc aatctttcat tgacacctat 781 atagatctgc ttgaaacacg cagaacttac tatgagggtc ccggagaggg tagcccgttc 841 ggttggaagg atatcaagga atggtatgaa atgttgatgg gacactgtac atacttcccg 901 gaagaacttc ggtcagtaaa atatgcatac aacgcagatc tttacaatgc cctgaatgat 961 ctgaataact tagtgatcac acgggacgag aatgaaaagt tggagtacta cgaaaagttc 1021 caaattattg aaaatgtgtt taagcaaaag aagaaaccta cactgaagca aattgccaaa 1081 gagatcttag tcaacgaaga ggacataaaa ggttatcgcg tcacctcgac cggcaaaccc 1141 gagttcacca atttgaaggt ttatcacgac ataaaggaca tcaccgcgag aaaagagatc 1201 atagagaacg cagagttact ggaccagatt gctaaaattt taaccatata tcagagtagc 1261 gaggacatac aggaagaatt aacgaatctt aatagtgagc ttacccagga ggagatcgag 1321 caaatttcga acctgaaggg atatacaggc acacacaacc tttcactgaa ggcgataaat 1381 ctgattttag acgaactgtg gcacacaaac gacaatcaaa ttgccatatt caatcgtctt 1441 aagttggtgc cgaaaaaagt cgatctgtcg cagcagaagg aaatccctac gaccttagta 1501 gatgatttta tcttaagccc cgtagttaag cgcagtttca ttcaaagcat aaaagtcata 1561 aatgcgatca tcaagaagta tgggcttccg aacgacataa ttatcgaatt ggctagagaa 1621 aagaactcga aagatgcgca aaagatgatc aacgagatgc aaaaacgcaa ccggcagacg 1681 aacgagcgta ttgaagagat cattagaacc acagggaaag aaaatgccaa gtatctgata 1741 gagaagatta aattacacga catgcaagaa ggaaaatgct tgtatagttt ggaggccatc 1801 ccgcttgagg atctgttgaa taatcctttc aactacgagg tggatcacat aataccccgt 1861 agcgtttcat tcgataattc ttttaataat aaggtacttg tcaagcagga ggagaatagt 1921 aaaaagggta accgcacgcc atttcaatat ttgtcctcgt ctgattcgaa aatttcgtac 1981 gaaaccttta agaaacacat tttgaacttg gcaaagggga aaggacggat ttcaaaaaca 2041 aagaaggaat accttttgga agaacgcgac ataaacagat ttagcgtgca gaaagacttt 2101 ataaaccgga acttagttga cactcggtac gctacgcggg gcttaatgaa ccttttacgg 2161 tcctacttcc gggttaataa tcttgatgtg aaagttaaga gcataaatgg tggatttaca 2221 tcgtttttgc ggcgtaaatg gaaattcaag aaggagcgga ataagggtta taaacaccac 2281 gcagaagatg cccttataat agcgaacgca gattttatct tcaaggaatg gaagaagctg 2341 gataaggcga agaaagtcat ggagaatcag atgtttgagg aaaaacaagc agagagcatg 2401 ccggaaatcg agacagagca ggagtataaa gagattttta taactcctca ccaaataaag 2461 catattaaag attttaaaga ttataaatat tcacatagag tggataaaaa acccaatcgg 2521 gaattaatca atgacacgtt gtatagtacc agaaaagacg ataaggggaa taccttaatt 2581 gtcaacaatt taaacggcct gtacgacaag gataacgata aacttaaaaa gctgatcaat 2641 aaaagcccag aaaaattgct gatgtaccat cacgaccctc agacctacca aaaattaaaa 2701 ttgattatgg aacaatacgg tgacgagaag aacccgttgt ataaatatta tgaagagacg 2761 ggcaattatt taactaaata ctcgaagaag gataacgggc ccgtcattaa gaagatcaaa 2821 tactatggca acaaattgaa tgcacatctt gatataaccg atgattaccc caattcacgc 2881 aataaagtag tcaaactgtc tttgaagccg taccggtttg atgtttactt ggataatggg 2941 gtgtataagt ttgtcacagt caaaaacctg gatgtcatta aaaaagaaaa ctactatgaa 3001 gtgaatagta aatgctacga agaggcaaaa aagcttaaaa agataagcaa tcaagcagaa 3061 tttatagcaa gtttctataa taatgatctt attaaaataa acggcgaact ttaccgcgtc 3121 ataggggtga ataacgacct tcttaataga atagaggtca atatgatcga tattacatac 3181 cgcgaatatt tagaaaatat gaatgataag cgcccccctc gcattatcaa gactatagca 3241 tccaagacgc agtccattaa gaaatactcc acggacatcc ttggcaacct ttacgaagtt 3301 aagtctaaaa aacatccgca gattattaaa aagggctga //