LOCUS BBa_K2019005 3282 bp DNA linear 23-Nov-2024 DEFINITION Periplasm Directed saCas9 via Ycdo Signaling Sequence (Ycdo-saCas9) ACCESSION BBa_K2019005 VERSION BBa_K2019005.1 FEATURES Location/Qualifiers ORIGIN 1 atgaccatta acttccgccg taacgcattg cagttgagcg tggctgcgct gttttcttct 61 gcttttatgg ctaacgccgc tgatgtgccg caggtcaaaa tgcaccacca tcatcatcat 121 atgaagagaa attacattct gggattagat ataggcatta ctagcgttgg gtacggaata 181 atcgactacg agacacgcga cgttatagac gctggcgtgc gcttgtttaa agaagctaat 241 gtggaaaaca atgaagggag acgtagtaaa cgcggtgccc gccgtttgaa aagaagacgc 301 cggcacagaa tacagcgcgt taagaagttg cttttcgact ataacttatt gacagaccat 361 tcagagcttt ctggtataaa cccctatgaa gcacgcgtta agggactttc tcagaagctt 421 tcggaagaag agttcagtgc cgcattgttg caccttgcaa agagacgtgg tgtccacaac 481 gtgaatgagg tcgaggaaga cacagggaat gaattgtcaa caaaagaaca gatatcacgg 541 aactcgaaag ccctggaaga aaaatacgtc gccgagttac agctggagcg gttgaagaag 601 gacggtgaag tacggggttc tataaatcgg ttcaaaacca gcgattacgt aaaagaagcg 661 aagcagcttc tgaaggtcca gaaagcatac caccagctgg accaatcttt cattgacacc 721 tatatagatc tgcttgaaac acgcagaact tactatgagg gtcccggaga gggtagcccg 781 ttcggttgga aggatatcaa ggaatggtat gaaatgttga tgggacactg tacatacttc 841 ccggaagaac ttcggtcagt aaaatatgca tacaacgcag atctttacaa tgccctgaat 901 gatctgaata acttagtgat cacacgggac gagaatgaaa agttggagta ctacgaaaag 961 ttccaaatta ttgaaaatgt gtttaagcaa aagaagaaac ctacactgaa gcaaattgcc 1021 aaagagatct tagtcaacga agaggacata aaaggttatc gcgtcacctc gaccggcaaa 1081 cccgagttca ccaatttgaa ggtttatcac gacataaagg acatcaccgc gagaaaagag 1141 atcatagaga acgcagagtt actggaccag attgctaaaa ttttaaccat atatcagagt 1201 agcgaggaca tacaggaaga attaacgaat cttaatagtg agcttaccca ggaggagatc 1261 gagcaaattt cgaacctgaa gggatataca ggcacacaca acctttcact gaaggcgata 1321 aatctgattt tagacgaact gtggcacaca aacgacaatc aaattgccat attcaatcgt 1381 cttaagttgg tgccgaaaaa agtcgatctg tcgcagcaga aggaaatccc tacgacctta 1441 gtagatgatt ttatcttaag ccccgtagtt aagcgcagtt tcattcaaag cataaaagtc 1501 ataaatgcga tcatcaagaa gtatgggctt ccgaacgaca taattatcga attggctaga 1561 gaaaagaact cgaaagatgc gcaaaagatg atcaacgaga tgcaaaaacg caaccggcag 1621 acgaacgagc gtattgaaga gatcattaga accacaggga aagaaaatgc caagtatctg 1681 atagagaaga ttaaattaca cgacatgcaa gaaggaaaat gcttgtatag tttggaggcc 1741 atcccgcttg aggatctgtt gaataatcct ttcaactacg aggtggatca cataataccc 1801 cgtagcgttt cattcgataa ttcttttaat aataaggtac ttgtcaagca ggaggagaat 1861 agtaaaaagg gtaaccgcac gccatttcaa tatttgtcct cgtctgattc gaaaatttcg 1921 tacgaaacct ttaagaaaca cattttgaac ttggcaaagg ggaaaggacg gatttcaaaa 1981 acaaagaagg aatacctttt ggaagaacgc gacataaaca gatttagcgt gcagaaagac 2041 tttataaacc ggaacttagt tgacactcgg tacgctacgc ggggcttaat gaacctttta 2101 cggtcctact tccgggttaa taatcttgat gtgaaagtta agagcataaa tggtggattt 2161 acatcgtttt tgcggcgtaa atggaaattc aagaaggagc ggaataaggg ttataaacac 2221 cacgcagaag atgcccttat aatagcgaac gcagatttta tcttcaagga atggaagaag 2281 ctggataagg cgaagaaagt catggagaat cagatgtttg aggaaaaaca agcagagagc 2341 atgccggaaa tcgagacaga gcaggagtat aaagagattt ttataactcc tcaccaaata 2401 aagcatatta aagattttaa agattataaa tattcacata gagtggataa aaaacccaat 2461 cgggaattaa tcaatgacac gttgtatagt accagaaaag acgataaggg gaatacctta 2521 attgtcaaca atttaaacgg cctgtacgac aaggataacg ataaacttaa aaagctgatc 2581 aataaaagcc cagaaaaatt gctgatgtac catcacgacc ctcagaccta ccaaaaatta 2641 aaattgatta tggaacaata cggtgacgag aagaacccgt tgtataaata ttatgaagag 2701 acgggcaatt atttaactaa atactcgaag aaggataacg ggcccgtcat taagaagatc 2761 aaatactatg gcaacaaatt gaatgcacat cttgatataa ccgatgatta ccccaattca 2821 cgcaataaag tagtcaaact gtctttgaag ccgtaccggt ttgatgttta cttggataat 2881 ggggtgtata agtttgtcac agtcaaaaac ctggatgtca ttaaaaaaga aaactactat 2941 gaagtgaata gtaaatgcta cgaagaggca aaaaagctta aaaagataag caatcaagca 3001 gaatttatag caagtttcta taataatgat cttattaaaa taaacggcga actttaccgc 3061 gtcatagggg tgaataacga ccttcttaat agaatagagg tcaatatgat cgatattaca 3121 taccgcgaat atttagaaaa tatgaatgat aagcgccccc ctcgcattat caagactata 3181 gcatccaaga cgcagtccat taagaaatac tccacggaca tccttggcaa cctttacgaa 3241 gttaagtcta aaaaacatcc gcagattatt aaaaagggct ga //