BBa_K2019005 1 BBa_K2019005 Periplasm Directed saCas9 via Ycdo Signaling Sequence (Ycdo-saCas9) 2016-10-11T11:00:00Z 2016-10-15T12:53:08Z Ycdo came from the paper " " Ycdo-Cas9 is a fusion of the Ycdo signaling sequence to the N-terminus of staphylococcus aureus CRISPR associated protein 9 (saCas9). Ycdo utilizes the sec pathway to direct the fused protein to the periplasm of the cell. Therefore, by fusing Cas9 to the C-terminus of the signaling sequence, it should be directed to the periplasm of the cell. false false _2486_ 31051 31051 9 false Ycdo was ordered as a gblock with homology to the BBa_K2019000-BBa_R0040 expression system. BBa_K2019000-BBa_R0040 was linearized at the 5' end of saCas9. A 2 piece gibson assembly was then performed with the gblock and Cas9 expression backbone. false John Lazar BBa_K2019005_sequence 1 atgaccattaacttccgccgtaacgcattgcagttgagcgtggctgcgctgttttcttctgcttttatggctaacgccgctgatgtgccgcaggtcaaaatgcaccaccatcatcatcatatgaagagaaattacattctgggattagatataggcattactagcgttgggtacggaataatcgactacgagacacgcgacgttatagacgctggcgtgcgcttgtttaaagaagctaatgtggaaaacaatgaagggagacgtagtaaacgcggtgcccgccgtttgaaaagaagacgccggcacagaatacagcgcgttaagaagttgcttttcgactataacttattgacagaccattcagagctttctggtataaacccctatgaagcacgcgttaagggactttctcagaagctttcggaagaagagttcagtgccgcattgttgcaccttgcaaagagacgtggtgtccacaacgtgaatgaggtcgaggaagacacagggaatgaattgtcaacaaaagaacagatatcacggaactcgaaagccctggaagaaaaatacgtcgccgagttacagctggagcggttgaagaaggacggtgaagtacggggttctataaatcggttcaaaaccagcgattacgtaaaagaagcgaagcagcttctgaaggtccagaaagcataccaccagctggaccaatctttcattgacacctatatagatctgcttgaaacacgcagaacttactatgagggtcccggagagggtagcccgttcggttggaaggatatcaaggaatggtatgaaatgttgatgggacactgtacatacttcccggaagaacttcggtcagtaaaatatgcatacaacgcagatctttacaatgccctgaatgatctgaataacttagtgatcacacgggacgagaatgaaaagttggagtactacgaaaagttccaaattattgaaaatgtgtttaagcaaaagaagaaacctacactgaagcaaattgccaaagagatcttagtcaacgaagaggacataaaaggttatcgcgtcacctcgaccggcaaacccgagttcaccaatttgaaggtttatcacgacataaaggacatcaccgcgagaaaagagatcatagagaacgcagagttactggaccagattgctaaaattttaaccatatatcagagtagcgaggacatacaggaagaattaacgaatcttaatagtgagcttacccaggaggagatcgagcaaatttcgaacctgaagggatatacaggcacacacaacctttcactgaaggcgataaatctgattttagacgaactgtggcacacaaacgacaatcaaattgccatattcaatcgtcttaagttggtgccgaaaaaagtcgatctgtcgcagcagaaggaaatccctacgaccttagtagatgattttatcttaagccccgtagttaagcgcagtttcattcaaagcataaaagtcataaatgcgatcatcaagaagtatgggcttccgaacgacataattatcgaattggctagagaaaagaactcgaaagatgcgcaaaagatgatcaacgagatgcaaaaacgcaaccggcagacgaacgagcgtattgaagagatcattagaaccacagggaaagaaaatgccaagtatctgatagagaagattaaattacacgacatgcaagaaggaaaatgcttgtatagtttggaggccatcccgcttgaggatctgttgaataatcctttcaactacgaggtggatcacataataccccgtagcgtttcattcgataattcttttaataataaggtacttgtcaagcaggaggagaatagtaaaaagggtaaccgcacgccatttcaatatttgtcctcgtctgattcgaaaatttcgtacgaaacctttaagaaacacattttgaacttggcaaaggggaaaggacggatttcaaaaacaaagaaggaataccttttggaagaacgcgacataaacagatttagcgtgcagaaagactttataaaccggaacttagttgacactcggtacgctacgcggggcttaatgaaccttttacggtcctacttccgggttaataatcttgatgtgaaagttaagagcataaatggtggatttacatcgtttttgcggcgtaaatggaaattcaagaaggagcggaataagggttataaacaccacgcagaagatgcccttataatagcgaacgcagattttatcttcaaggaatggaagaagctggataaggcgaagaaagtcatggagaatcagatgtttgaggaaaaacaagcagagagcatgccggaaatcgagacagagcaggagtataaagagatttttataactcctcaccaaataaagcatattaaagattttaaagattataaatattcacatagagtggataaaaaacccaatcgggaattaatcaatgacacgttgtatagtaccagaaaagacgataaggggaataccttaattgtcaacaatttaaacggcctgtacgacaaggataacgataaacttaaaaagctgatcaataaaagcccagaaaaattgctgatgtaccatcacgaccctcagacctaccaaaaattaaaattgattatggaacaatacggtgacgagaagaacccgttgtataaatattatgaagagacgggcaattatttaactaaatactcgaagaaggataacgggcccgtcattaagaagatcaaatactatggcaacaaattgaatgcacatcttgatataaccgatgattaccccaattcacgcaataaagtagtcaaactgtctttgaagccgtaccggtttgatgtttacttggataatggggtgtataagtttgtcacagtcaaaaacctggatgtcattaaaaaagaaaactactatgaagtgaatagtaaatgctacgaagaggcaaaaaagcttaaaaagataagcaatcaagcagaatttatagcaagtttctataataatgatcttattaaaataaacggcgaactttaccgcgtcataggggtgaataacgaccttcttaatagaatagaggtcaatatgatcgatattacataccgcgaatatttagaaaatatgaatgataagcgcccccctcgcattatcaagactatagcatccaagacgcagtccattaagaaatactccacggacatccttggcaacctttacgaagttaagtctaaaaaacatccgcagattattaaaaagggctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z