LOCUS       BBa_K2020002            1439 bp    DNA     linear       25-Nov-2024
DEFINITION  expression system for subtilisin E in E. coli
ACCESSION   BBa_K2020002
VERSION     BBa_K2020002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..292
                     /label=PelB
     misc_feature    227..292
                     /label=BBa_J32015
     CDS             293..522
                     /label=propeptide
     CDS             523..1351
                     /label=subtilisin E
     misc_feature    293..1351
                     /label=BBa_K2020000
     CDS             227..1351
                     /label=BBa_K2020001
     stem_loop       1371..1414
                     /label=stem_loop
     misc_feature    1360..1439
                     /label=BBa_B0010
     terminator      1360..1439
                     /label=BBa_B0010
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aatacctgct
      241 gccgaccgct gctgctggtc tgctgctcct cgctgcccag ccggcgatgg ccgcgggcaa
      301 aagcagcacc gaaaaaaaat atattgtggg ctttaaacag accatgagcg cgatgagcag
      361 cgcgaaaaaa aaagatgtga ttagcgaaaa aggcggcaaa gtgcagaaac agtttaaata
      421 tgtgaacgcg gcggcggcga ccctggatga aaaagcggtg aaagaactga aaaaagatcc
      481 gagcgtggcg tatgtggaag aagatcatat cgcgcatgaa tatgcccaga gcgtgccgta
      541 tggcattagc cagattaaag ccccggcgct gcatagccag ggctataccg gcagcaacgt
      601 gaaagtggcg gtgattgata gcggcattga tagcagccat ccggatctga acgtgcgcgg
      661 cggcgcgagc tttgtgccga gcgaaaccaa cccgtatcag gatggcagca gccatggcac
      721 ccatgtggcg ggcaccattg cggcgctgaa caacagcatt ggcgtgctgg gcgtgagccc
      781 gagcgcgagc ctgtatgcgg tgaaagtgct ggatagcacc ggcagcggcc agtatagctg
      841 gattattaac ggcattgaat gggcgattag caacaacatg gatgtgatta acatgagcct
      901 gggcggcccg accggcagca ccgcgctgaa aaccgtggtg gataaagcgg tgagcagcgg
      961 cattgtggtg gcggcggcgg cgggcaacga aggcagcagc ggcagcacca gcaccgtggg
     1021 ctatccggcg aaatatccgt caacgattgc tgttggcgcc gtaaattcaa gcaatcagcg
     1081 tgcgtcattc tcatccgcag gtagcgaact ggatgtgatg gcgccgggcg tgagcattca
     1141 gagcaccctg ccgggcggca cctatggcgc gtataacggc accagcatgg cgaccccgca
     1201 tgtggcgggc gcggcggcgc tgattctgag caaacatccg acctggacca acgcgcaggt
     1261 gcgcgatcgc ctggaaagca ccgcgaccta tctgggcaac agcttttatt atggcaaagg
     1321 cctgattaac gtgcaggcgg cggcgcagta atactagagc caggcatcaa ataaaacgaa
     1381 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctc
//