LOCUS       BBa_K2020004            1439 bp    DNA     linear       22-Dec-2024
DEFINITION  mutated expression system for subtilisin E in E. coli (S221X)
ACCESSION   BBa_K2020004
VERSION     BBa_K2020004.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=LacI
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..291
                     /label=PelB
     CDS             293..522
                     /label=propeptide
     CDS             523..1351
                     /label=subtilisin E
     misc_feature    1182..1184
                     /label=AGC1182TAG
     misc_feature    1360..1439
                     /label=BBa_B0010
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aatacctgct
      241 gccgaccgct gctgctggtc tgctgctcct cgctgcccag ccggcgatgg ccgcgggcaa
      301 aagcagcacc gaaaaaaaat atattgtggg ctttaaacag accatgagcg cgatgagcag
      361 cgcgaaaaaa aaagatgtga ttagcgaaaa aggcggcaaa gtgcagaaac agtttaaata
      421 tgtgaacgcg gcggcggcga ccctggatga aaaagcggtg aaagaactga aaaaagatcc
      481 gagcgtggcg tatgtggaag aagatcatat cgcgcatgaa tatgcccaga gcgtgccgta
      541 tggcattagc cagattaaag ccccggcgct gcatagccag ggctataccg gcagcaacgt
      601 gaaagtggcg gtgattgata gcggcattga tagcagccat ccggatctga acgtgcgcgg
      661 cggcgcgagc tttgtgccga gcgaaaccaa cccgtatcag gatggcagca gccatggcac
      721 ccatgtggcg ggcaccattg cggcgctgaa caacagcatt ggcgtgctgg gcgtgagccc
      781 gagcgcgagc ctgtatgcgg tgaaagtgct ggatagcacc ggcagcggcc agtatagctg
      841 gattattaac ggcattgaat gggcgattag caacaacatg gatgtgatta acatgagcct
      901 gggcggcccg accggcagca ccgcgctgaa aaccgtggtg gataaagcgg tgagcagcgg
      961 cattgtggtg gcggcggcgg cgggcaacga aggcagcagc ggcagcacca gcaccgtggg
     1021 ctatccggcg aaatatccgt caacgattgc tgttggcgcc gtaaattcaa gcaatcagcg
     1081 tgcgtcattc tcatccgcag gtagcgaact ggatgtgatg gcgccgggcg tgagcattca
     1141 gagcaccctg ccgggcggca cctatggcgc gtataacggc acctagatgg cgaccccgca
     1201 tgtggcgggc gcggcggcgc tgattctgag caaacatccg acctggacca acgcgcaggt
     1261 gcgcgatcgc ctggaaagca ccgcgaccta tctgggcaac agcttttatt atggcaaagg
     1321 cctgattaac gtgcaggcgg cggcgcagta atactagagc caggcatcaa ataaaacgaa
     1381 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctc
//