LOCUS       BBa_K2020040            1437 bp    DNA     linear       25-Nov-2024
DEFINITION  Fluorescent reporter for measurement of incorporation of ncAA
ACCESSION   BBa_K2020040
VERSION     BBa_K2020040.1  
FEATURES             Location/Qualifiers
     CDS             1..674
                     /label=mRFP1
     misc_feature    678..711
                     /label=modified Waldo et al linker
     misc_feature    712..714
                     /label=TAG
     misc_feature    1435..1437
                     /label=TAA
     CDS             715..1434
                     /label=sfGFP
ORIGIN
        1 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
       61 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      121 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      181 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      241 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      301 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      361 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      421 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      481 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      541 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      601 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      661 cactccaccg gtgctatggg atccgctggc tccgctgctg gttctggcga ataggcatcc
      721 aagggcgagg agctctttac tggcgtagta ccaattctcg tagagctcga tggcgatgta
      781 aatggccata agttttccgt acgcggcgag ggcgagggcg atgcaactaa cggcaagctc
      841 actctcaagt ttatttgtac tactggcaag ctcccagtac catggccaac tctcgtaact
      901 actctgacct atggcgtaca atgtttttcc cgctatccag atcacatgaa gcaacatgat
      961 ttttttaagt ccgcaatgcc agagggctat gtacaagagc gcactattag ctttaaggat
     1021 gatggcacct ataagactcg cgcagaggta aagtttgagg gcgatactct cgtaaatcgc
     1081 attgagctca agggcattga ttttaaggag gatggcaata ttctcggcca taagctggag
     1141 tataatttca attcccataa tgtatatatt accgcagata agcaaaagaa tggcattaag
     1201 gcgaatttta agattcgcca taatgtggag gatggctccg tacaactcgc agatcattat
     1261 caacaaaata ctccaattgg cgatggccca gtactcctcc cagataatca ttatctctcc
     1321 actcaatccg tgctctccaa agatccaaat gagaagcgcg atcacatggt actcctggag
     1381 tttgtaacgg cagcaggcat tactcatggc atggatgagc tctataagct cgagtaa
//