BBa_K2020046 1 BBa_K2020046 CNF-Synthetase for use in E.coli 2016-10-14T11:00:00Z 2016-12-02T08:49:58Z Obtained via iGEM Team Austin, Texas 2014 This is the 4-Cyano-L-phenylalanine-synthetase to be used as a orthogonal synthetase in E.coli. This part can be used together with the [[Part:BBa_K2020042|cognate tRNA BBa_K2020042]] to incorporate CNF in response to an amber stop codon. false false _2487_ 30647 29923 9 false Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs. [http://2016.igem.org/Team:Aachen Team Aachen 2016] used similar synthetases in pACYC with p15A-ORI and Gent-Resistance. Together with a tRNA (BBa_K2020042) with own Ipp-Promoter and Terminator. false Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher BBa_K2020046_sequence 1 atggacgagttcgagatgatcaaacgcaacaccagcgaaatcatcagcgaagaagaactgcgcgaagtgctgaaaaaagacgagaagagcgccctgattggcttcgaaccgagcggcaaaattcatctgggccactacctgccgatcaagaagatgatcgatctgccgaacgcgggcttcgatatcatcattgtgctggccgatctgcatgcatacctgaaccagaaaggcgaactggacgaaatccgcaaaatcggcgactacaacaagaaagtgttcgaagcgatgggcctgaaagcgaaatacgtctatggcagcgaatggatgctggacaaagactacaccctgaacgtgtatcgtctggcactgaaaaccaccctgaaacgtgctcgtcgttctatggaactgattgcccgcgaagatgaaaacccgaaagtggcggaagtgatctatccgatcatgcaggtgaacggcgcgcactatctgggcgtagatgtagcagttggtggcatggaacagcgcaaaatccacatgctggctcgtgaactgctgccgaaaaaagtcgtgtgcatccacaacccggttttaacgggtctggatggtgaaggcaaaatgagcagcagcaaaggcaactttatcgccgtggatgattctccggaagaaatccgcgcgaagatcaaaaaagcgtattgcccggcaggtgtagttgaaggtaacccgatcatggagatcgcgaaatacttcctggaatacccgctgaccattaaacgcccggaaaaattcggtggtgatctgaccgtgaacagctacgaagaactggagagcctgttcaagaacaaagaactgcacccgatgcgcctgaaaaatgccgttgcggaagagctgatcaaaatcctggaaccgattcgcaaacgtctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z