BBa_K2020050 1 BBa_K2020050 wild type Mj Y-Synthetase for use in E.coli 2016-10-01T11:00:00Z 2016-12-19T03:13:18Z wild type tyrosyl Methanococcus janaschii tRNA-Synthetase. Obtained via iGEM Team 2014 Austin, Texas. This is the wild type tyrosyl synthetase derived from Methanococcus janaschii to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate Tyrosin in response to an amber stop codon. tbc false false _2487_ 30647 29923 9 false Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs. [http://2016.igem.org/Team:Aachen Team Aachen 2016] used synthetases in pACYC with p15A-ORI and Gent-Resistance. Together with a tRNA (BBa_K2020042) with own Ipp-Promoter and Terminator. false Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher BBa_K2020050_sequence 1 atggacgagttcgagatgatcaaacgcaacaccagcgaaatcatcagcgaagaagaactgcgcgaagtgctgaaaaaagacgagaagagcgcctatattggcttcgaaccgagcggcaaaattcatctgggccactacctgccgatcaagaagatgatcgatctgccgaacgcgggcttcgatatcatcattctgctggccgatctgcatgcatacctgaaccagaaaggcgaactggacgaaatccgcaaaatcggcgactacaacaagaaagtgttcgaagcgatgggcctgaaagcgaaatacgtctatggcagcgaatttcagctggacaaagactacaccctgaacgtgtatcgtctggcactgaaaaccaccctgaaacgtgctcgtcgttctatggaactgattgcccgcgaagatgaaaacccgaaagtggcggaagtgatctatccgatcatgcaggtgaacgacatccactatctgggcgtagatgtagcagttggtggcatggaacagcgcaaaatccacatgctggctcgtgaactgctgccgaaaaaagtcgtgtgcatccacaacccggttttaacgggtctggatggtgaaggcaaaatgagcagcagcaaaggcaactttatcgccgtggatgattctccggaagaaatccgcgcgaagatcaaaaaagcgtattgcccggcaggtgtagttgaaggtaacccgatcatggagatcgcgaaatacttcctggaatacccgctgaccattaaacgcccggaaaaattcggtggtgatctgaccgtgaacagctacgaagaactggagagcctgttcaagaacaaagaactgcacccgatgcgtctgaaaaatgccgttgcggaagagctgatcaaaatcctggaaccgattcgcaaacgtctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z