BBa_K2020058 1 BBa_K2020058 DMNBS-Synthetase for use in E.coli 2016-10-14T11:00:00Z 2016-12-02T08:54:46Z Basis for mutation was Methanococcus janaschii wild type tyrosyl synthetase. This is a DMNBS-synthetase to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate DMNBS in response to an amber stop codon. false false _2487_ 30647 29923 9 false The synthetase was created via mutation library following computational design from Methanococcus janaschii wild type tyrosyl synthetase. false Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher annotation2525364 1 DMNBS-RS range2525364 1 1 921 BBa_K2020058_sequence 1 atggacgagttcgagatgatcaaacgcaacaccagcgaaatcatcagcgaagaagaactgcgcgaagtgctgaaaaaagacgagaagagcgccggtattggcttcgaaccgagcggcaaaattcatctgggccactacctgccgatcaagaagatgatcgatctgccgaacgcgggcttcgatatcatcattggtctggccgatctgcatgcatacctgaaccagaaaggcgaactggacgaaatccgcaaaatcggcgactacaacaagaaagtgttcgaagcgatgggcctgaaagcgaaatacgtctatggcagcgaatttcagctggacaaagactacaccctgaacgtgtatcgtctggcactgaaaaccaccctgaaacgtgctcgtcgttctatggaactgattgcccgcgaagatgaaaacccgaaagtggcggaagtgatctatccgatcatgcaggtgaacgccagccactatctgggcgtagatgtagcagttggtggcatggaacagcgcaaagttgctatgctggctcgtgaactgctgccgaaaaaagtcgtgtgcatccacaacccggttttaacgggtctggatggtgaaggcaaaatgagcagcagcaaaggcaactttatcgccgtggatgattctccggaagaaatccgcgcgaagatcaaaaaagcgtattgcccggcaggtgtagttgaaggtaacccgatcatggagatcgcgaaatacttcctggaatacccgctgaccattaaacgcccggaaaaattcggtggtgatctgaccgtgaacagctacgaagaactggagagcctgttcaagaacaaagaactgcacccgatgcgtctgaaaaatgccgttgcggaagagctgatcaaaatcctggaaccgattcgcaaacgtctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z