LOCUS       BBa_K2022008             445 bp    DNA     linear       25-Nov-2024
DEFINITION  T7 Expression Construct for tolR, Periplasmic Domain
ACCESSION   BBa_K2022008
VERSION     BBa_K2022008.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     CDS             39..269
                     /label=BBa_K2022002
     stem_loop       289..332
                     /label=stem_loop
     misc_feature    278..357
                     /label=BBa_B0010
     terminator      278..357
                     /label=BBa_B0010
     stem_loop       377..420
                     /label=stem_loop
     misc_feature    366..445
                     /label=BBa_B0010
     terminator      366..445
                     /label=BBa_B0010
     terminator      278..445
                     /label=BBa_B0017
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat ggaggtcgat ctgccagacg
       61 ctactgaatc acaggcggtg agcagtaacg ataatccgcc agtgattgtt gaagtgtctg
      121 gtattggtca gtacaccgtg gtggttgaga aagatcgcct ggagcgttta ccaccagagc
      181 aggtggtggc ggaagtgtcc agccgtttca aggccaaccc gaaaacggtc tttctgatcg
      241 gtggcgcaaa agatgtgcct tactaataat actagagcca ggcatcaaat aaaacgaaag
      301 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      361 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      421 ctgttgtttg tcggtgaacg ctctc
//