LOCUS       BBa_K2023001            3261 bp    DNA     linear       22-Dec-2024
DEFINITION  Biosensor device for BTEX detection
ACCESSION   BBa_K2023001
VERSION     BBa_K2023001.1  
FEATURES             Location/Qualifiers
     promoter        1..410
                     /label=Pr
     promoter        1..410
                     /label=BBa_I723018
     misc_feature    423..426
                     /label=conserved
     RBS             419..430
                     /label=BBa_B0034
     CDS             437..2140
                     /label=XylR
     CDS             437..2140
                     /label=BBa_K2023005
     stem_loop       2156..2175
                     /label=T7 TE
     misc_feature    2182..2182
                     /label=stop
     polyA_site      2176..2189
                     /label=polya
     misc_feature    2149..2189
                     /label=BBa_B0012
     terminator      2149..2189
                     /label=BBa_B0012
     stem_loop       2210..2232
                     /label=stem_loop
     misc_feature    2198..2243
                     /label=BBa_B0011
     terminator      2198..2243
                     /label=BBa_B0011
     terminator      2149..2243
                     /label=BBa_B0014
     promoter        1..2243
                     /label=BBa_K2023002
     promoter        2252..2571
                     /label=Pu
     promoter        2252..2571
                     /label=BBa_I723020
     misc_feature    2584..2587
                     /label=conserved
     RBS             2580..2591
                     /label=BBa_B0034
     CDS             2598..3158
                     /label=GLuc
     CDS             2598..3158
                     /label=BBa_K2023009
     stem_loop       3174..3193
                     /label=T7 TE
     misc_feature    3200..3200
                     /label=stop
     polyA_site      3194..3207
                     /label=polya
     misc_feature    3167..3207
                     /label=BBa_B0012
     terminator      3167..3207
                     /label=BBa_B0012
     stem_loop       3228..3250
                     /label=stem_loop
     misc_feature    3216..3261
                     /label=BBa_B0011
     terminator      3216..3261
                     /label=BBa_B0011
     terminator      3167..3261
                     /label=BBa_B0014
     misc_feature    2252..3261
                     /label=BBa_K2023003
ORIGIN
        1 attttaatgt gggctgcttg gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct
       61 cgacgtgatg cgtatacggg ttacccccat tgccacgttg cgccatcctt tttgcaatca
      121 gtgaccactt ttccaagcaa aaataacgcc aagcagaacg aagacgttct ttttaagaag
      181 cgagaacacc agaagttcgt gctgtcgggg catgcggcga cgaattggcg gataaagggg
      241 atctgcgttg aggtggattt cagttaatca attggttaat ctttcaggac cacctaagca
      301 aatgctaaag tggcagatgg aatgctgagc cggcaagcac aggccttgac gttgcaaggt
      361 agtcatgacc gcagtgagcc tctgatgttc cgccgggtgg atcatcccga tactagagaa
      421 agaggagaaa tactagatgt cacttattta taaacctaag atgcaacacg aagacatgca
      481 ggatcttatt tcccaaatcc gttttgtcgc tgccgaggga aaaatttggc tgggagaaca
      541 gcgtatgttg cttatgcaac tttcgacgtt ggcatcattt cggagagaaa taatatcctt
      601 gataggcata gaaagagcga agggcttttt cttacggctt gggtatcaga gtggattgat
      661 ggacgcagaa ttggctcgta aactgcgccc ggccatgcgt gaagaagagg tattccttgc
      721 cggtccgcaa ctgtatgcgt tgaaaggcat ggtgaaagtc agattgttga ccatggatat
      781 agcaatcaga gatggcagat tcaatgtcga agcggagtgg atagactcct tcgaagtgga
      841 tatttgtcgg acggagctgg ggctgatgaa tgagccagtg tgctggactg tgttaggata
      901 cgcaagtgga tacggtagtg cctttatggg tcgcagaata attttccagg aaaccagttg
      961 tcgtggctgc ggtgacgaca aatgccttat tgtgggtaag acggcagagg aatgggggga
     1021 tgtatcctcg tttgaagctt actttaaaag cgacccgata gtggatgagc ggtacgaact
     1081 ccagacacag gttgctaatc tgagaaacag acttaaacag tatgacggcc agtactacgg
     1141 gataggtcat tcacctgcat ataaacgcat ctgtgagaca atagacaaag ccgctcgcgg
     1201 taaggtatca gtcttactgt tgggcgagac gggcgttgga aaagaagtaa tagcacggag
     1261 tgtccactta cgctccgaga gagcggagca gccgttcgtg gcggttaatt gcgctgcaat
     1321 tcctccggat ttaattgagt ctgaactttt cggcgtggat aagggagctt atacgggggc
     1381 ggttaatgcg cgggctggaa gattcgaacg tgcaaatggg ggcacaatct tcttggatga
     1441 ggtcatagaa cttacgcccc gcgcgcaggc cactttgctg cgggttttgc aggagggaga
     1501 gctggaaaga gtggggggcg atagaacacg taaagtggac gttagattaa ttaccgctac
     1561 caatgaaaac ttggaggaag ctgtgaaaat gggtagattt cgggcggatc tgttcttccg
     1621 gcttaatgtt tttccggttc agattccacc ccttcgcgag cgggttgagg acatacccct
     1681 tttagtagag cattttctgc gtcgtcatca caaagagtat ggtaaaaaga cccttgggtt
     1741 atctgacaga gccatggagg catgccttca ctaccagtgg cctggcaaca tacgtgaatt
     1801 ggagaacgcg ctggagcgtg gtgtaattct gacggaatcg aatgaaagta taaatgtgga
     1861 aagcttattc ccagggcttg cgatggctac tgaaggtgat agactttcta gcgagggacg
     1921 gcttgaagag gaatcgggag attcatggtt cagacagata atagaccaag gcgtatctct
     1981 ggaagatttg gaagcaggac ttatgagaac cgccatggac agatgcgggc aaaatatatc
     2041 tcaggccgca cgtttgcttg gcttgaccag acccgcaatg gcgtatcgct taaaaaaact
     2101 ggatcctagt ttgtcagtca aggctacggg gcgttaatag tactagagtc acactggctc
     2161 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
     2221 ttaatccggc ttttttatta ttttactaga gcccgggaaa gcgcgatgaa ccttttttat
     2281 cgctgccttg atcaaatcga caggtggtta tgcgcgattg atgatttgct caaatacagc
     2341 cagcgtgctg tagattttct ctcatacccc ccctttcttt tttacaaaga aaatcaataa
     2401 tttagatgaa ataaggggat cggtataagc aatggcatgg cggttgctag ctatacgaga
     2461 cttaaaataa aaatagtggt gacccttcaa tgttgtattt tctcaactct gttcagattg
     2521 gttgctttcg ccatgtatat cctcaaagcg ggccagccgt agccgttacg ctactagaga
     2581 aagaggagaa atactagatg ggagtgaaag ttctttttgc ccttatttgt attgctgtgg
     2641 ccgaggccaa accaactgaa aacaatgaag atttcaacat tgtagctgta gctagcaact
     2701 ttgctacaac ggatctcgat gctgaccgtg gtaaattgcc cggaaaaaaa ttaccacttg
     2761 aggtactcaa agaaatggaa gccaatgcta ggaaagctgg ctgcactagg ggatgtctga
     2821 tatgcctgtc acacatcaag tgtacaccca aaatgaagaa gtttatccca ggaagatgcc
     2881 acacctatga aggagacaaa gaaagtgcac agggaggaat aggagaggct attgttgaca
     2941 ttcctgaaat tcctgggttt aaggatttgg aacccatgga acaattcatt gcacaagttg
     3001 acctatgtgt agactgcaca actggatgcc tcaaaggtct tgccaatgtg caatgttctg
     3061 atttactcaa gaaatggctg ccacaaagat gtgcaacttt tgctagcaaa attcaaggcc
     3121 aagtggacaa aataaagggt gccggtggtg attaatagta ctagagtcac actggctcac
     3181 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
     3241 aatccggctt ttttattatt t
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